Cervical duct cannulation in sheep for collection of afferent lymph dendritic cells from head tissues View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Isabelle Schwartz-Cornil, Mathieu Epardaud, Michel Bonneau

ABSTRACT

Pseudo-afferent cervical lymph-duct cannulation in a sheep model allows large amounts of lymph cells to be collected under physiological conditions, carrying immune signaling information from the head tissues, including oro-nasal mucosae. Importantly, large quantities of dendritic cells (DCs) of several subtypes are obtained (up to 8 million per overnight collection), as well as many other trafficking leukocytes. The technique includes three steps: removal of all head lymph nodes on one side (2 h), catheterization of cervical lymph ducts after 2 months (2-3 h) and collection/purification of lymph-cell subsets (4 h). The approach is challenging (1 in 3 success rate) but fruitful, and can be used to study DC subsets under immunomodulation, in order to assess lymph-cell subset dynamic changes and antigen transportation from oro-nasal tissues. This protocol is directed to experienced postdoctoral researchers. More... »

PAGES

874-879

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nprot.2006.147

DOI

http://dx.doi.org/10.1038/nprot.2006.147

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046809833

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17406320


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