Tuning membrane protein mobility by confinement into nanodomains View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-03

AUTHORS

Andreas Karner, Benedikt Nimmervoll, Birgit Plochberger, Enrico Klotzsch, Andreas Horner, Denis G. Knyazev, Roland Kuttner, Klemens Winkler, Lukas Winter, Christine Siligan, Nicole Ollinger, Peter Pohl, Johannes Preiner

ABSTRACT

High-speed atomic force microscopy (HS-AFM) can be used to visualize function-related conformational changes of single soluble proteins. Similar studies of single membrane proteins are, however, hampered by a lack of suitable flat, non-interacting membrane supports and by high protein mobility. Here we show that streptavidin crystals grown on mica-supported lipid bilayers can be used as porous supports for membranes containing biotinylated lipids. Using SecYEG (protein translocation channel) and GlpF (aquaglyceroporin), we demonstrate that the platform can be used to tune the lateral mobility of transmembrane proteins to any value within the dynamic range accessible to HS-AFM imaging through glutaraldehyde-cross-linking of the streptavidin. This allows HS-AFM to study the conformation or docking of spatially confined proteins, which we illustrate by imaging GlpF at sub-molecular resolution and by observing the motor protein SecA binding to SecYEG. More... »

PAGES

260-266

References to SciGraph publications

  • 2008-04. Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2014-02. Structure of the SecY channel during initiation of protein translocation in NATURE
  • 2014-12. IgGs are made for walking on bacterial and viral surfaces in NATURE COMMUNICATIONS
  • 2006-12. Two-chamber AFM: probing membrane proteins separating two aqueous compartments in NATURE METHODS
  • 2002-08. Three-dimensional structure of the bacterial protein-translocation complex SecYEG in NATURE
  • 2010-11. Video imaging of walking myosin V by high-speed atomic force microscopy in NATURE
  • 2012-08. Characterization of the motion of membrane proteins using high-speed atomic force microscopy in NATURE NANOTECHNOLOGY
  • 2015-12. Resolving single membrane fusion events on planar pore-spanning membranes in SCIENTIFIC REPORTS
  • 2008-10. Structure of a complex of the ATPase SecA and the protein-translocation channel in NATURE
  • 2011-05. Cryo-EM structure of the ribosome–SecYE complex in the membrane environment in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2010-06. Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates in NATURE PROTOCOLS
  • 2014-12. Alarmin S100A8/S100A9 as a biomarker for molecular imaging of local inflammatory activity in NATURE COMMUNICATIONS
  • 2010-05. Fast, single-molecule localization that achieves theoretically minimum uncertainty in NATURE METHODS
  • 2005-09. Polymer-supported membranes as models of the cell surface in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nnano.2016.236

    DOI

    http://dx.doi.org/10.1038/nnano.2016.236

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020979010

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27842062


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lipid Bilayers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Membrane Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Atomic Force", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Domains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Transport", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Center for Advanced Bioanalysis GmbH, Gruberstrasse 40\u201342, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Karner", 
            "givenName": "Andreas", 
            "id": "sg:person.01101417654.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101417654.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Center for Advanced Bioanalysis GmbH, Gruberstrasse 40\u201342, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nimmervoll", 
            "givenName": "Benedikt", 
            "id": "sg:person.01300124147.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300124147.81"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Upper Austria University of Applied Sciences, Campus Linz, Garnisonstrasse 21, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Plochberger", 
            "givenName": "Birgit", 
            "id": "sg:person.0723164744.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723164744.91"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "UNSW Australia", 
              "id": "https://www.grid.ac/institutes/grid.1005.4", 
              "name": [
                "EMBL Australia Node in Single Molecule Science and ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Klotzsch", 
            "givenName": "Enrico", 
            "id": "sg:person.01315306372.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315306372.87"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Horner", 
            "givenName": "Andreas", 
            "id": "sg:person.01314143172.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314143172.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Knyazev", 
            "givenName": "Denis G.", 
            "id": "sg:person.0775506260.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775506260.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kuttner", 
            "givenName": "Roland", 
            "id": "sg:person.015306162401.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015306162401.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Winkler", 
            "givenName": "Klemens", 
            "id": "sg:person.016103543001.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016103543001.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Winter", 
            "givenName": "Lukas", 
            "id": "sg:person.01342411660.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342411660.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Siligan", 
            "givenName": "Christine", 
            "id": "sg:person.0764650651.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764650651.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ollinger", 
            "givenName": "Nicole", 
            "id": "sg:person.01160050060.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160050060.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Johannes Kepler University of Linz", 
              "id": "https://www.grid.ac/institutes/grid.9970.7", 
              "name": [
                "Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pohl", 
            "givenName": "Peter", 
            "id": "sg:person.01275577034.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275577034.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Center for Advanced Bioanalysis GmbH, Gruberstrasse 40\u201342, 4020 Linz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Preiner", 
            "givenName": "Johannes", 
            "id": "sg:person.01227056500.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227056500.59"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1126/sciadv.1400083", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000498747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0806451105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001714722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nnano.2012.109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001908655", 
              "https://doi.org/10.1038/nnano.2012.109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.surfrep.2006.06.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003541181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(10)75020-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005230186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1106377109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006841583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2013.04.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008746044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011518474", 
              "https://doi.org/10.1038/nsmb.2026"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.211400898", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012353001"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2010.07.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014939763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m114.588491", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016251349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017575936", 
              "https://doi.org/10.1038/nmeth.1449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1449", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017575936", 
              "https://doi.org/10.1038/nmeth.1449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep12006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017890670", 
              "https://doi.org/10.1038/srep12006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cbpa.2007.09.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018540864"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1415261112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019494061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0969-2126(00)00065-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020923852"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(90)90742-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023223782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2013.07.052", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024327063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m303490200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024590449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560070420", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024692744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560070420", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024692744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025574149", 
              "https://doi.org/10.1038/nature07335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2010.65", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025653052", 
              "https://doi.org/10.1038/nprot.2010.65"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04164", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025677274", 
              "https://doi.org/10.1038/nature04164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04164", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025677274", 
              "https://doi.org/10.1038/nature04164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04164", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025677274", 
              "https://doi.org/10.1038/nature04164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04164", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025677274", 
              "https://doi.org/10.1038/nature04164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09450", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030990737", 
              "https://doi.org/10.1038/nature09450"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09450", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030990737", 
              "https://doi.org/10.1038/nature09450"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/nl504478f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031684266"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2009.07.046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032153770"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0052839", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034463322"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth965", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034630408", 
              "https://doi.org/10.1038/nmeth965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth965", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034630408", 
              "https://doi.org/10.1038/nmeth965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-3495(97)78139-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035930434"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature00827", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036271572", 
              "https://doi.org/10.1038/nature00827"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature00827", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036271572", 
              "https://doi.org/10.1038/nature00827"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444911027806", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036365938"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2009.04.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037830081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms5394", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039294503", 
              "https://doi.org/10.1038/ncomms5394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00424-007-0409-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040151914", 
              "https://doi.org/10.1007/s00424-007-0409-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m113.477893", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042877627"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-3495(76)85755-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046177476"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1205510", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046397104"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1364/oe.17.004685", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046821743"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2008.06.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047356227"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2008.06.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047356227"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms5593", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047784326", 
              "https://doi.org/10.1038/ncomms5593"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bc20040136", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048642381"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2005.06.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049009340"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049272029", 
              "https://doi.org/10.1038/nature12720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi048263j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055199033"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi048263j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055199033"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la010626i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056142675"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la010626i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056142675"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la061934p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056150935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la061934p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056150935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la970590n", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056168771"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la970590n", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056168771"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physreve.74.051911", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060735277"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physreve.74.051911", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060735277"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physrevlett.94.238104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060830505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physrevlett.94.238104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060830505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.290.5491.481", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062571790"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-03", 
        "datePublishedReg": "2017-03-01", 
        "description": "High-speed atomic force microscopy (HS-AFM) can be used to visualize function-related conformational changes of single soluble proteins. Similar studies of single membrane proteins are, however, hampered by a lack of suitable flat, non-interacting membrane supports and by high protein mobility. Here we show that streptavidin crystals grown on mica-supported lipid bilayers can be used as porous supports for membranes containing biotinylated lipids. Using SecYEG (protein translocation channel) and GlpF (aquaglyceroporin), we demonstrate that the platform can be used to tune the lateral mobility of transmembrane proteins to any value within the dynamic range accessible to HS-AFM imaging through glutaraldehyde-cross-linking of the streptavidin. This allows HS-AFM to study the conformation or docking of spatially confined proteins, which we illustrate by imaging GlpF at sub-molecular resolution and by observing the motor protein SecA binding to SecYEG.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nnano.2016.236", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6208142", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1037429", 
            "issn": [
              "1748-3387", 
              "1748-3395"
            ], 
            "name": "Nature Nanotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "name": "Tuning membrane protein mobility by confinement into nanodomains", 
        "pagination": "260-266", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "63f49334301ceee3f0931b4f6cd0dc826daa6f0ee27198e12d25c2c29b07d367"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27842062"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101283273"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nnano.2016.236"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1020979010"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nnano.2016.236", 
          "https://app.dimensions.ai/details/publication/pub.1020979010"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T23:11", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000424.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nnano.2016.236"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nnano.2016.236'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nnano.2016.236'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nnano.2016.236'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nnano.2016.236'


     

    This table displays all metadata directly associated to this object as RDF triples.

    358 TRIPLES      21 PREDICATES      86 URIs      28 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nnano.2016.236 schema:about N16f0c5ebc7f844dc89dd5cf75f7dbce4
    2 N258da2ca62e34328be1d48c3df55a8ca
    3 N2b008a60e69547a7871b9b5a72ad97d5
    4 N44751f7f565d4ad288e45724743cd38c
    5 N9a7c0490289648cab906d08842fd4744
    6 Na437feaed36740f9a320b28aa854260f
    7 Ne2caabbd0e764017a76e7c8e6e8b909e
    8 anzsrc-for:06
    9 anzsrc-for:0601
    10 schema:author N6489d5a490a3417cb396bc974af66a51
    11 schema:citation sg:pub.10.1007/s00424-007-0409-x
    12 sg:pub.10.1038/nature00827
    13 sg:pub.10.1038/nature04164
    14 sg:pub.10.1038/nature07335
    15 sg:pub.10.1038/nature09450
    16 sg:pub.10.1038/nature12720
    17 sg:pub.10.1038/ncomms5394
    18 sg:pub.10.1038/ncomms5593
    19 sg:pub.10.1038/nmeth.1449
    20 sg:pub.10.1038/nmeth965
    21 sg:pub.10.1038/nnano.2012.109
    22 sg:pub.10.1038/nprot.2010.65
    23 sg:pub.10.1038/nsmb.2026
    24 sg:pub.10.1038/srep12006
    25 https://doi.org/10.1002/pro.5560070420
    26 https://doi.org/10.1016/0092-8674(90)90742-w
    27 https://doi.org/10.1016/j.bbamem.2013.04.028
    28 https://doi.org/10.1016/j.bpj.2009.07.046
    29 https://doi.org/10.1016/j.bpj.2010.07.028
    30 https://doi.org/10.1016/j.bpj.2013.07.052
    31 https://doi.org/10.1016/j.cbpa.2007.09.020
    32 https://doi.org/10.1016/j.jsb.2005.06.002
    33 https://doi.org/10.1016/j.jsb.2009.04.011
    34 https://doi.org/10.1016/j.surfrep.2006.06.001
    35 https://doi.org/10.1016/j.ymeth.2008.06.010
    36 https://doi.org/10.1016/s0006-3495(76)85755-4
    37 https://doi.org/10.1016/s0006-3495(97)78139-6
    38 https://doi.org/10.1016/s0076-6879(10)75020-5
    39 https://doi.org/10.1016/s0969-2126(00)00065-4
    40 https://doi.org/10.1021/bi048263j
    41 https://doi.org/10.1021/la010626i
    42 https://doi.org/10.1021/la061934p
    43 https://doi.org/10.1021/la970590n
    44 https://doi.org/10.1021/nl504478f
    45 https://doi.org/10.1042/bc20040136
    46 https://doi.org/10.1073/pnas.0806451105
    47 https://doi.org/10.1073/pnas.1106377109
    48 https://doi.org/10.1073/pnas.1415261112
    49 https://doi.org/10.1073/pnas.211400898
    50 https://doi.org/10.1074/jbc.m113.477893
    51 https://doi.org/10.1074/jbc.m114.588491
    52 https://doi.org/10.1074/jbc.m303490200
    53 https://doi.org/10.1103/physreve.74.051911
    54 https://doi.org/10.1103/physrevlett.94.238104
    55 https://doi.org/10.1107/s0907444911027806
    56 https://doi.org/10.1126/sciadv.1400083
    57 https://doi.org/10.1126/science.1205510
    58 https://doi.org/10.1126/science.290.5491.481
    59 https://doi.org/10.1364/oe.17.004685
    60 https://doi.org/10.1371/journal.pone.0052839
    61 schema:datePublished 2017-03
    62 schema:datePublishedReg 2017-03-01
    63 schema:description High-speed atomic force microscopy (HS-AFM) can be used to visualize function-related conformational changes of single soluble proteins. Similar studies of single membrane proteins are, however, hampered by a lack of suitable flat, non-interacting membrane supports and by high protein mobility. Here we show that streptavidin crystals grown on mica-supported lipid bilayers can be used as porous supports for membranes containing biotinylated lipids. Using SecYEG (protein translocation channel) and GlpF (aquaglyceroporin), we demonstrate that the platform can be used to tune the lateral mobility of transmembrane proteins to any value within the dynamic range accessible to HS-AFM imaging through glutaraldehyde-cross-linking of the streptavidin. This allows HS-AFM to study the conformation or docking of spatially confined proteins, which we illustrate by imaging GlpF at sub-molecular resolution and by observing the motor protein SecA binding to SecYEG.
    64 schema:genre research_article
    65 schema:inLanguage en
    66 schema:isAccessibleForFree true
    67 schema:isPartOf N6d699c854d164b6eb34e32b0a1d551fc
    68 Nc6fed4658fe949b188b5e9da008b9d66
    69 sg:journal.1037429
    70 schema:name Tuning membrane protein mobility by confinement into nanodomains
    71 schema:pagination 260-266
    72 schema:productId N36125f1532944ef8a60821146f244df6
    73 N5154851291724cd3b19699139962717d
    74 N82db2be44dfe463fa5321ba6323689b4
    75 Ncb73d4f8ac844e0782a9ceac9120f472
    76 Nf99d546a7da143fc9f06d74cd87ab024
    77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020979010
    78 https://doi.org/10.1038/nnano.2016.236
    79 schema:sdDatePublished 2019-04-10T23:11
    80 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    81 schema:sdPublisher Ndb81c9bbe7d3427596040a96a5264b82
    82 schema:url http://www.nature.com/articles/nnano.2016.236
    83 sgo:license sg:explorer/license/
    84 sgo:sdDataset articles
    85 rdf:type schema:ScholarlyArticle
    86 N093b118db171431d8f6464c1e05a1766 rdf:first sg:person.0775506260.66
    87 rdf:rest N6c12731878cb47039e0da194e749985b
    88 N16f0c5ebc7f844dc89dd5cf75f7dbce4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Protein Transport
    90 rdf:type schema:DefinedTerm
    91 N19ddd24f914747269b6324d584ea94eb rdf:first sg:person.016103543001.45
    92 rdf:rest Nae955c8c881a4e769347c64b9e298035
    93 N258da2ca62e34328be1d48c3df55a8ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Lipid Bilayers
    95 rdf:type schema:DefinedTerm
    96 N2b008a60e69547a7871b9b5a72ad97d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Protein Domains
    98 rdf:type schema:DefinedTerm
    99 N2db67d3b22bc4f7e93fd1fcc2e4eb3aa rdf:first sg:person.01227056500.59
    100 rdf:rest rdf:nil
    101 N337cda9f74744fd4a360fa1893956f1d rdf:first sg:person.0723164744.91
    102 rdf:rest Nf230326a7aa442f2ac2a22e8c304af75
    103 N36125f1532944ef8a60821146f244df6 schema:name nlm_unique_id
    104 schema:value 101283273
    105 rdf:type schema:PropertyValue
    106 N44751f7f565d4ad288e45724743cd38c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Microscopy, Atomic Force
    108 rdf:type schema:DefinedTerm
    109 N5154851291724cd3b19699139962717d schema:name readcube_id
    110 schema:value 63f49334301ceee3f0931b4f6cd0dc826daa6f0ee27198e12d25c2c29b07d367
    111 rdf:type schema:PropertyValue
    112 N6489d5a490a3417cb396bc974af66a51 rdf:first sg:person.01101417654.63
    113 rdf:rest Nf1a7d50463a342b29c81f74a98f61dd6
    114 N6c12731878cb47039e0da194e749985b rdf:first sg:person.015306162401.42
    115 rdf:rest N19ddd24f914747269b6324d584ea94eb
    116 N6d699c854d164b6eb34e32b0a1d551fc schema:issueNumber 3
    117 rdf:type schema:PublicationIssue
    118 N764b37808cba4ca0a8354b939cdf7097 schema:name Center for Advanced Bioanalysis GmbH, Gruberstrasse 40–42, 4020 Linz, Austria
    119 rdf:type schema:Organization
    120 N82db2be44dfe463fa5321ba6323689b4 schema:name doi
    121 schema:value 10.1038/nnano.2016.236
    122 rdf:type schema:PropertyValue
    123 N97febfed8fd34451bafe9fa9cbea4f6e schema:name Upper Austria University of Applied Sciences, Campus Linz, Garnisonstrasse 21, 4020 Linz, Austria
    124 rdf:type schema:Organization
    125 N98ced31cb34049818ae78a5e0d71c78b rdf:first sg:person.01275577034.20
    126 rdf:rest N2db67d3b22bc4f7e93fd1fcc2e4eb3aa
    127 N9a7c0490289648cab906d08842fd4744 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Escherichia coli Proteins
    129 rdf:type schema:DefinedTerm
    130 Na437feaed36740f9a320b28aa854260f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Escherichia coli
    132 rdf:type schema:DefinedTerm
    133 Naa0a84c8aacf404a939a497ee7e44d56 schema:name Center for Advanced Bioanalysis GmbH, Gruberstrasse 40–42, 4020 Linz, Austria
    134 rdf:type schema:Organization
    135 Nae955c8c881a4e769347c64b9e298035 rdf:first sg:person.01342411660.17
    136 rdf:rest Nc821ecc9002a4eddb0e8bb33e06348ca
    137 Nc2fdfeddb90f4babad8767ffd4c89c9a rdf:first sg:person.01314143172.16
    138 rdf:rest N093b118db171431d8f6464c1e05a1766
    139 Nc6fed4658fe949b188b5e9da008b9d66 schema:volumeNumber 12
    140 rdf:type schema:PublicationVolume
    141 Nc821ecc9002a4eddb0e8bb33e06348ca rdf:first sg:person.0764650651.30
    142 rdf:rest Ne1e119b4e58a441eb7f431d6b7349c93
    143 Ncb73d4f8ac844e0782a9ceac9120f472 schema:name pubmed_id
    144 schema:value 27842062
    145 rdf:type schema:PropertyValue
    146 Ndb81c9bbe7d3427596040a96a5264b82 schema:name Springer Nature - SN SciGraph project
    147 rdf:type schema:Organization
    148 Ne1e119b4e58a441eb7f431d6b7349c93 rdf:first sg:person.01160050060.63
    149 rdf:rest N98ced31cb34049818ae78a5e0d71c78b
    150 Ne2caabbd0e764017a76e7c8e6e8b909e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Membrane Proteins
    152 rdf:type schema:DefinedTerm
    153 Neb967d1bfd0b4303b4285bd503c64b70 schema:name Center for Advanced Bioanalysis GmbH, Gruberstrasse 40–42, 4020 Linz, Austria
    154 rdf:type schema:Organization
    155 Nf1a7d50463a342b29c81f74a98f61dd6 rdf:first sg:person.01300124147.81
    156 rdf:rest N337cda9f74744fd4a360fa1893956f1d
    157 Nf230326a7aa442f2ac2a22e8c304af75 rdf:first sg:person.01315306372.87
    158 rdf:rest Nc2fdfeddb90f4babad8767ffd4c89c9a
    159 Nf99d546a7da143fc9f06d74cd87ab024 schema:name dimensions_id
    160 schema:value pub.1020979010
    161 rdf:type schema:PropertyValue
    162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    163 schema:name Biological Sciences
    164 rdf:type schema:DefinedTerm
    165 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    166 schema:name Biochemistry and Cell Biology
    167 rdf:type schema:DefinedTerm
    168 sg:grant.6208142 http://pending.schema.org/fundedItem sg:pub.10.1038/nnano.2016.236
    169 rdf:type schema:MonetaryGrant
    170 sg:journal.1037429 schema:issn 1748-3387
    171 1748-3395
    172 schema:name Nature Nanotechnology
    173 rdf:type schema:Periodical
    174 sg:person.01101417654.63 schema:affiliation Naa0a84c8aacf404a939a497ee7e44d56
    175 schema:familyName Karner
    176 schema:givenName Andreas
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101417654.63
    178 rdf:type schema:Person
    179 sg:person.01160050060.63 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    180 schema:familyName Ollinger
    181 schema:givenName Nicole
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160050060.63
    183 rdf:type schema:Person
    184 sg:person.01227056500.59 schema:affiliation N764b37808cba4ca0a8354b939cdf7097
    185 schema:familyName Preiner
    186 schema:givenName Johannes
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227056500.59
    188 rdf:type schema:Person
    189 sg:person.01275577034.20 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    190 schema:familyName Pohl
    191 schema:givenName Peter
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275577034.20
    193 rdf:type schema:Person
    194 sg:person.01300124147.81 schema:affiliation Neb967d1bfd0b4303b4285bd503c64b70
    195 schema:familyName Nimmervoll
    196 schema:givenName Benedikt
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300124147.81
    198 rdf:type schema:Person
    199 sg:person.01314143172.16 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    200 schema:familyName Horner
    201 schema:givenName Andreas
    202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314143172.16
    203 rdf:type schema:Person
    204 sg:person.01315306372.87 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
    205 schema:familyName Klotzsch
    206 schema:givenName Enrico
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315306372.87
    208 rdf:type schema:Person
    209 sg:person.01342411660.17 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    210 schema:familyName Winter
    211 schema:givenName Lukas
    212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342411660.17
    213 rdf:type schema:Person
    214 sg:person.015306162401.42 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    215 schema:familyName Kuttner
    216 schema:givenName Roland
    217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015306162401.42
    218 rdf:type schema:Person
    219 sg:person.016103543001.45 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    220 schema:familyName Winkler
    221 schema:givenName Klemens
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016103543001.45
    223 rdf:type schema:Person
    224 sg:person.0723164744.91 schema:affiliation N97febfed8fd34451bafe9fa9cbea4f6e
    225 schema:familyName Plochberger
    226 schema:givenName Birgit
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723164744.91
    228 rdf:type schema:Person
    229 sg:person.0764650651.30 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    230 schema:familyName Siligan
    231 schema:givenName Christine
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764650651.30
    233 rdf:type schema:Person
    234 sg:person.0775506260.66 schema:affiliation https://www.grid.ac/institutes/grid.9970.7
    235 schema:familyName Knyazev
    236 schema:givenName Denis G.
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775506260.66
    238 rdf:type schema:Person
    239 sg:pub.10.1007/s00424-007-0409-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040151914
    240 https://doi.org/10.1007/s00424-007-0409-x
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1038/nature00827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036271572
    243 https://doi.org/10.1038/nature00827
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1038/nature04164 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025677274
    246 https://doi.org/10.1038/nature04164
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1038/nature07335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025574149
    249 https://doi.org/10.1038/nature07335
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1038/nature09450 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030990737
    252 https://doi.org/10.1038/nature09450
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1038/nature12720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049272029
    255 https://doi.org/10.1038/nature12720
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1038/ncomms5394 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039294503
    258 https://doi.org/10.1038/ncomms5394
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/ncomms5593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047784326
    261 https://doi.org/10.1038/ncomms5593
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/nmeth.1449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017575936
    264 https://doi.org/10.1038/nmeth.1449
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1038/nmeth965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034630408
    267 https://doi.org/10.1038/nmeth965
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1038/nnano.2012.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001908655
    270 https://doi.org/10.1038/nnano.2012.109
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1038/nprot.2010.65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025653052
    273 https://doi.org/10.1038/nprot.2010.65
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1038/nsmb.2026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011518474
    276 https://doi.org/10.1038/nsmb.2026
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/srep12006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017890670
    279 https://doi.org/10.1038/srep12006
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1002/pro.5560070420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024692744
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1016/0092-8674(90)90742-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1023223782
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1016/j.bbamem.2013.04.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008746044
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1016/j.bpj.2009.07.046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032153770
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1016/j.bpj.2010.07.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014939763
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1016/j.bpj.2013.07.052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024327063
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1016/j.cbpa.2007.09.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018540864
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1016/j.jsb.2005.06.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049009340
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1016/j.jsb.2009.04.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037830081
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1016/j.surfrep.2006.06.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003541181
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1016/j.ymeth.2008.06.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047356227
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1016/s0006-3495(76)85755-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046177476
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1016/s0006-3495(97)78139-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035930434
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1016/s0076-6879(10)75020-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005230186
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1016/s0969-2126(00)00065-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020923852
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.1021/bi048263j schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199033
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.1021/la010626i schema:sameAs https://app.dimensions.ai/details/publication/pub.1056142675
    314 rdf:type schema:CreativeWork
    315 https://doi.org/10.1021/la061934p schema:sameAs https://app.dimensions.ai/details/publication/pub.1056150935
    316 rdf:type schema:CreativeWork
    317 https://doi.org/10.1021/la970590n schema:sameAs https://app.dimensions.ai/details/publication/pub.1056168771
    318 rdf:type schema:CreativeWork
    319 https://doi.org/10.1021/nl504478f schema:sameAs https://app.dimensions.ai/details/publication/pub.1031684266
    320 rdf:type schema:CreativeWork
    321 https://doi.org/10.1042/bc20040136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048642381
    322 rdf:type schema:CreativeWork
    323 https://doi.org/10.1073/pnas.0806451105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001714722
    324 rdf:type schema:CreativeWork
    325 https://doi.org/10.1073/pnas.1106377109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006841583
    326 rdf:type schema:CreativeWork
    327 https://doi.org/10.1073/pnas.1415261112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019494061
    328 rdf:type schema:CreativeWork
    329 https://doi.org/10.1073/pnas.211400898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012353001
    330 rdf:type schema:CreativeWork
    331 https://doi.org/10.1074/jbc.m113.477893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042877627
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1074/jbc.m114.588491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016251349
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1074/jbc.m303490200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024590449
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1103/physreve.74.051911 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060735277
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1103/physrevlett.94.238104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060830505
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1107/s0907444911027806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036365938
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1126/sciadv.1400083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000498747
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1126/science.1205510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046397104
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1126/science.290.5491.481 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062571790
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1364/oe.17.004685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046821743
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1371/journal.pone.0052839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034463322
    352 rdf:type schema:CreativeWork
    353 https://www.grid.ac/institutes/grid.1005.4 schema:alternateName UNSW Australia
    354 schema:name EMBL Australia Node in Single Molecule Science and ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, Australia
    355 rdf:type schema:Organization
    356 https://www.grid.ac/institutes/grid.9970.7 schema:alternateName Johannes Kepler University of Linz
    357 schema:name Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria
    358 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...