The microglial sensome revealed by direct RNA sequencing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Suzanne E Hickman, Nathan D Kingery, Toshiro K Ohsumi, Mark L Borowsky, Li-chong Wang, Terry K Means, Joseph El Khoury

ABSTRACT

Microglia, the principal neuroimmune sentinels of the brain, continuously sense changes in their environment and respond to invading pathogens, toxins and cellular debris. Microglia exhibit plasticity and can assume neurotoxic or neuroprotective priming states that determine their responses to danger. We used direct RNA sequencing, without amplification or cDNA synthesis, to determine the quantitative transcriptomes of microglia of healthy adult and aged mice. We validated our findings using fluorescence dual in situ hybridization, unbiased proteomic analysis and quantitative PCR. We found that microglia have a distinct transcriptomic signature and express a unique cluster of transcripts encoding proteins for sensing endogenous ligands and microbes that we refer to as the sensome. With aging, sensome transcripts for endogenous ligand recognition were downregulated, whereas those involved in microbe recognition and host defense were upregulated. In addition, aging was associated with an overall increase in the expression of microglial genes involved in neuroprotection. More... »

PAGES

1896-1905

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nn.3554

    DOI

    http://dx.doi.org/10.1038/nn.3554

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1016133129

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24162652


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