Molecular Architecture of the Chick Vestibular Hair Bundle View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-01-20

AUTHORS

Jung-Bum Shin, Jocelyn F. Krey, Ahmed Hassan, Zoltan Metlagel, Andrew N. Tauscher, James M. Pagana, Nicholas E. Sherman, Erin D. Jeffery, Kateri J. Spinelli, Hongyu Zhao, Phillip A. Wilmarth, Dongseok Choi, Larry L. David, Manfred Auer, Peter G. Barr-Gillespie

ABSTRACT

Hair bundles of the inner ear have a specialized structure and protein composition that underlies their sensitivity to mechanical stimulation. Using mass spectrometry, we identified and quantified >1,100 proteins, present from a few to 400,000 copies per stereocilium, from purified chick bundles; 336 of these were significantly enriched in bundles. Bundle proteins that we detected have been shown to regulate cytoskeleton structure and dynamics, energy metabolism, phospholipid synthesis and cell signaling. Three-dimensional imaging using electron tomography allowed us to count the number of actin-actin cross-linkers and actin-membrane connectors; these values compared well to those obtained from mass spectrometry. Network analysis revealed several hub proteins, including RDX (radixin) and SLC9A3R2 (NHERF2), which interact with many bundle proteins and may perform functions essential for bundle structure and function. The quantitative mass spectrometry of bundle proteins reported here establishes a framework for future characterization of dynamic processes that shape bundle structure and function. More... »

PAGES

365-374

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nn.3312

    DOI

    http://dx.doi.org/10.1038/nn.3312

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025263864

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23334578


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