SCALEs: multiscale analysis of library enrichment View Full Text


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Article Info

DATE

2007-01

AUTHORS

Michael D Lynch, Tanya Warnecke, Ryan T Gill

ABSTRACT

We report a genome-wide, multiscale approach to simultaneously measure the effect that the increased copy of each gene and/or operon has on a desired trait or phenotype. The method involves (i) growth selections on a mixture of several different plasmid-based genomic libraries of defined insert sizes or SCALEs, (ii) microarray studies of enriched plasmid DNA, and a (iii) mathematical multiscale analysis that precisely identifies the relevant genetic elements. This approach allows for identification of all single open reading frames and larger multigene fragments within a genomic library that alter the expression of a given phenotype. We have demonstrated this method in Escherichia coli by monitoring, in parallel, a population of >10(6) genomic library clones of different insert sizes, throughout continuous selections over a period of 100 generations. More... »

PAGES

87

Journal

TITLE

Nature Methods

ISSUE

1

VOLUME

4

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth946

    DOI

    http://dx.doi.org/10.1038/nmeth946

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17099705


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