Ontology type: schema:ScholarlyArticle Open Access: True
2006-10
AUTHORSMichael J Rust, Mark Bates, Xiaowei Zhuang
ABSTRACTWe have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution. More... »
PAGES793-796
http://scigraph.springernature.com/pub.10.1038/nmeth929
DOIhttp://dx.doi.org/10.1038/nmeth929
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