Feeder-independent culture of human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Tenneille E Ludwig, Veit Bergendahl, Mark E Levenstein, Junying Yu, Mitchell D Probasco, James A Thomson

ABSTRACT

We recently reported the development of TeSR1, a serum-free, animal product–free medium that supports the derivation and long-term feeder-independent culture of human embryonic stem cells1. Although the derivation of new human embryonic stem cell lines in those defined conditions offered an important proof of principle, the costs of some of the defined components in that culture system made it impractical for everyday research use. Here we describe modifications to the medium (mTeSR1) that include the use of animal-sourced proteins (bovine serum albumin (BSA) and Matrigel) and cloned zebrafish basic fibroblast growth factor (zbFGF). We include a simple protocol that allows purification of up to 100 mg zbFGF in less than three days (Fig. 1), an amount sufficient to make 1,000 l of mTeSR1 medium. The modifications presented here make mTeSR1 practical for routine research use, and the protocols presented are those currently used in our laboratory for standard human embryonic stem cell culture. More... »

PAGES

637-646

Journal

TITLE

Nature Methods

ISSUE

8

VOLUME

3

Related Patents

  • Predicting Human Developmental Toxicity Of Pharmaceuticals Using Human Stem-Like Cells And Metabolomics
  • Fgf Having Enhanced Stability
  • Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Methods And Products For Transfection
  • Simplified Basic Media For Human Pluripotent Cell Culture
  • New Serum-Free Medium For Inducing Pluripotent Stem Cells Quickly With High Efficiency And Method Using Thereof
  • Xeno-Free Generation Of Tissue-Specific Progenitor Cells
  • Methods And Products For Transfecting Cells
  • Methods And Compositions For Somatic Cell Proliferation And Viability
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Systems For Converting Precursor Cells Into Intestinal Tissues Through Directed Differentiation
  • Thermostable Fgf-2 Mutant Having Enhanced Stability
  • Hemangio Colony Forming Cells And Non-Engrafting Hemangio Cells
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Methods For Producing Hepatocytes
  • Substrates And Methods For Culturing Stem Cells
  • Clonal Culture Of Human Pluripotent Stem Cells
  • Methods And Products For Expressing Proteins In Cells
  • Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Microfluidic Cell Trap And Assay Apparatus For High-Throughput Analysis
  • A Method Of Preparing Isolated Cells And Uses
  • Methods For Culturing Undifferentiated Cells Using Sustained Release Compositions
  • Cell Culture Substrate Comprising A Laminin And A Cadherin
  • Cell Culture Composition Comprising Laminin-521
  • Methods And Products For Expressing Proteins In Cells
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Microfluidic Cell Trap And Assay Apparatus For High-Throughput Analysis
  • Culture Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid Or Lithium For The Maintenance Of Stem Cells In An Undifferentiated State
  • Methods And Products For Transfecting Cells
  • Methods And Products For Nucleic Acid Production And Delivery
  • Method For Isolation Of Inner Cell Mass And Method Of Preparation Of Embryonic Stem Cell Lines Using Inner Cell Mass Isolated By The Same
  • Human Blood-Brain Endothelial Cells Derived From Pluripotent Stem Cells And Blood-Brain Barrier Model Thereof
  • Manipulation Of Osmolality For Differentiating Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Methods For Producing Enucleated Erythroid Cells Derived From Pluripotent Stem Cells
  • Automated Method And Apparatus For Embryonic Stem Cell Culture
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Primate Pluripotent Stem Cells Cultured In Medium Containing Gamma-Aminobutyric Acid, Pipecolic Acid And Lithium
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Differentiation Of Pluripotent Cells
  • Vitronectin-Derived Cell Culture Substrate And Uses Thereof
  • Membrane-Separation-Type Culture Device, Membrane-Separation-Type Culture Kit, Stem Cell Separation Method Using Same, And Separation Membrane
  • Methods And Products For Expressing Proteins In Cells
  • Chitosan-Alginate Scaffold Cell Culture System And Related Methods
  • Fgf Having Enhanced Stability
  • New Serum-Free Medium For Inducing Pluripotent Stem Cells Quickly With High Efficiency And Method Using Thereof
  • Composition And Method For Proliferating Undifferentiated Stem Cells
  • Methods And Products For Transfection
  • Medium Comprising Transforming Growth Factor Β1 And Basic Fibroblast Growth Factor
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Simplified Basic Media For Human Pluripotent Cell Culture
  • Methods For The Production Of Ips Cells Using Non-Viral Approach
  • Reprogramming Immortalized B-Cells To Induced Pluripotent Stem Cells
  • Methods And Products For Transfection
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Methods And Compositions For The Differentiation Of Stem Cells
  • Mass Spectrometry Data Acquisition Mode For Obtaining More Reliable Protein Quantitation
  • Reagents And Methods For Using Human Embryonic Stem Cells To Evaluate Toxicity Of Pharmaceutical Compounds And Other Chemicals
  • Predicting Human Developmental Toxicity Of Pharmaceuticals Using Human Stem-Like Cells And Metabolomics
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Products For Transfecting Cells
  • Methods For The Production Of Ips Cells
  • Methods And Products For Expressing Proteins In Cells
  • Method For Proliferating Hair Follicle Stem Cells
  • Method For Rejuvenating Cells
  • Methods For Culturing Undifferentiated Cells Using Sustained Release Compositions
  • Xeno-Free Generation Of Tissue-Specific Progenitor Cells
  • Reprogramming Cells To A Less Differentiated State
  • Methods Of Reprogramming Cells To A Less Differentiated State
  • Methods And Products For Reprogramming Cells To A Less Differentiated State
  • Gas-Phase Purification For Accurate Isobaric Tag-Based Quantification
  • Products For Transfection And Reprogramming
  • Methods For Culturing Undifferentiated Cells Using Sustained Release Compositions
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth902

    DOI

    http://dx.doi.org/10.1038/nmeth902

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019814710

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16862139


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Proliferation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Survival", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tissue Engineering", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Wisconsin\u2013Madison", 
              "id": "https://www.grid.ac/institutes/grid.14003.36", 
              "name": [
                "Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA.", 
                "WiCell Research Institute, P.O. Box 7365, Madison, Wisconsin 53707, USA.", 
                "National Primate Research Center, University of Wisconsin Graduate School, 1220 Capitol Court, Madison, Wisconsin 53705, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ludwig", 
            "givenName": "Tenneille E", 
            "id": "sg:person.01154664726.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Wisconsin\u2013Madison", 
              "id": "https://www.grid.ac/institutes/grid.14003.36", 
              "name": [
                "Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bergendahl", 
            "givenName": "Veit", 
            "id": "sg:person.01016530662.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016530662.81"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "WiCell", 
              "id": "https://www.grid.ac/institutes/grid.439113.d", 
              "name": [
                "WiCell Research Institute, P.O. Box 7365, Madison, Wisconsin 53707, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Levenstein", 
            "givenName": "Mark E", 
            "id": "sg:person.01145062633.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Wisconsin\u2013Madison", 
              "id": "https://www.grid.ac/institutes/grid.14003.36", 
              "name": [
                "National Primate Research Center, University of Wisconsin Graduate School, 1220 Capitol Court, Madison, Wisconsin 53705, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Junying", 
            "id": "sg:person.01336707071.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336707071.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Wisconsin\u2013Madison", 
              "id": "https://www.grid.ac/institutes/grid.14003.36", 
              "name": [
                "Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Probasco", 
            "givenName": "Mitchell D", 
            "id": "sg:person.01056475774.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056475774.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Wisconsin\u2013Madison", 
              "id": "https://www.grid.ac/institutes/grid.14003.36", 
              "name": [
                "Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA.", 
                "WiCell Research Institute, P.O. Box 7365, Madison, Wisconsin 53707, USA.", 
                "National Primate Research Center, University of Wisconsin Graduate School, 1220 Capitol Court, Madison, Wisconsin 53705, USA.", 
                "Department of Anatomy, University of Wisconsin Medical School, 470 N. Charter Street, Madison, Wisconsin 53706, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Thomson", 
            "givenName": "James A", 
            "id": "sg:person.012266000337.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.ydbio.2004.08.031", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001805072"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/01483918308064922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002094798"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt922", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005956847", 
              "https://doi.org/10.1038/nbt922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod.103.021147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007519570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0279", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008652652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2004-10-4065", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011381855"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021378655", 
              "https://doi.org/10.1038/nbt1189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021378655", 
              "https://doi.org/10.1038/nbt1189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029326356", 
              "https://doi.org/10.1038/nmeth744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030407065", 
              "https://doi.org/10.1038/nbt1177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030407065", 
              "https://doi.org/10.1038/nbt1177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/dev.01706", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033196590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2005-0247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036210003"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2005-0247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036210003"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1197", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037634240", 
              "https://doi.org/10.1038/nbt1197"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1197", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037634240", 
              "https://doi.org/10.1038/nbt1197"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02631084", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039710839", 
              "https://doi.org/10.1007/bf02631084"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2004-0211", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045135667"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0021-9673(96)00286-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049184527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2006-08", 
        "datePublishedReg": "2006-08-01", 
        "description": "We recently reported the development of TeSR1, a serum-free, animal product\u2013free medium that supports the derivation and long-term feeder-independent culture of human embryonic stem cells1. Although the derivation of new human embryonic stem cell lines in those defined conditions offered an important proof of principle, the costs of some of the defined components in that culture system made it impractical for everyday research use. Here we describe modifications to the medium (mTeSR1) that include the use of animal-sourced proteins (bovine serum albumin (BSA) and Matrigel) and cloned zebrafish basic fibroblast growth factor (zbFGF). We include a simple protocol that allows purification of up to 100 mg zbFGF in less than three days (Fig. 1), an amount sufficient to make 1,000 l of mTeSR1 medium. The modifications presented here make mTeSR1 practical for routine research use, and the protocols presented are those currently used in our laboratory for standard human embryonic stem cell culture.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nmeth902", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2437849", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2622167", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "3"
          }
        ], 
        "name": "Feeder-independent culture of human embryonic stem cells", 
        "pagination": "637-646", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "5e688f1955b36e75e488b323d480fcd442c88f86e3742aff197b5e158a22edce"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16862139"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101215604"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth902"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1019814710"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth902", 
          "https://app.dimensions.ai/details/publication/pub.1019814710"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:23", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87091_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nmeth902"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth902'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth902'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth902'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth902'


     

    This table displays all metadata directly associated to this object as RDF triples.

    204 TRIPLES      21 PREDICATES      54 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth902 schema:about N12827911bd434e7581a9ab23a5e8fcbf
    2 N15ea027a85bd462d962a7bf17aef4ff4
    3 N2883d02f301e4dd9b9d1026bcea2f6f9
    4 N40c8e312ac6c4d04862ebf58f05a8def
    5 N96f310be265e4019b5923c170fd29ac1
    6 N9b2da03a2ed7497a9c22a510512efc77
    7 Nc536ba7f4bb0468aad3b9af186046f12
    8 Nd10c9b6a9e3248b6a9977d0051452851
    9 Ne64c6a53f16a4f129310c7bbbdbc8a29
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author Nc6526077dcc54cd0874b4afdffd07b68
    13 schema:citation sg:pub.10.1007/bf02631084
    14 sg:pub.10.1038/nbt1177
    15 sg:pub.10.1038/nbt1189
    16 sg:pub.10.1038/nbt1197
    17 sg:pub.10.1038/nbt922
    18 sg:pub.10.1038/nmeth744
    19 https://doi.org/10.1006/dbio.2000.9912
    20 https://doi.org/10.1016/0021-9673(96)00286-5
    21 https://doi.org/10.1016/j.ydbio.2004.08.031
    22 https://doi.org/10.1080/01483918308064922
    23 https://doi.org/10.1095/biolreprod.103.021147
    24 https://doi.org/10.1182/blood-2004-10-4065
    25 https://doi.org/10.1242/dev.01706
    26 https://doi.org/10.1634/stemcells.2004-0211
    27 https://doi.org/10.1634/stemcells.2004-0279
    28 https://doi.org/10.1634/stemcells.2005-0247
    29 schema:datePublished 2006-08
    30 schema:datePublishedReg 2006-08-01
    31 schema:description We recently reported the development of TeSR1, a serum-free, animal product–free medium that supports the derivation and long-term feeder-independent culture of human embryonic stem cells1. Although the derivation of new human embryonic stem cell lines in those defined conditions offered an important proof of principle, the costs of some of the defined components in that culture system made it impractical for everyday research use. Here we describe modifications to the medium (mTeSR1) that include the use of animal-sourced proteins (bovine serum albumin (BSA) and Matrigel) and cloned zebrafish basic fibroblast growth factor (zbFGF). We include a simple protocol that allows purification of up to 100 mg zbFGF in less than three days (Fig. 1), an amount sufficient to make 1,000 l of mTeSR1 medium. The modifications presented here make mTeSR1 practical for routine research use, and the protocols presented are those currently used in our laboratory for standard human embryonic stem cell culture.
    32 schema:genre research_article
    33 schema:inLanguage en
    34 schema:isAccessibleForFree false
    35 schema:isPartOf N310ba73eafd94bb9b16b1c3a3892444c
    36 Nee299b4a109a4b00a4035ed0e889fcd3
    37 sg:journal.1033763
    38 schema:name Feeder-independent culture of human embryonic stem cells
    39 schema:pagination 637-646
    40 schema:productId N15201614a5fb4f4bbf16bda4947c496c
    41 N2ed9cbdee2f9491e8ace47e988712cf6
    42 N486ea65a3c2d46c49c5b6bee757ded96
    43 N4f49cb7736124d9c8079519ebd12434d
    44 N8ef6a815cd604f8f8b218f217aefdcd6
    45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019814710
    46 https://doi.org/10.1038/nmeth902
    47 schema:sdDatePublished 2019-04-11T12:23
    48 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    49 schema:sdPublisher N143f78a703dd4b2d9cbf7bd213eb1cb8
    50 schema:url http://www.nature.com/articles/nmeth902
    51 sgo:license sg:explorer/license/
    52 sgo:sdDataset articles
    53 rdf:type schema:ScholarlyArticle
    54 N0d0dbbce45114ffcba49bc4d3645ee33 rdf:first sg:person.01056475774.13
    55 rdf:rest N74e2fe6ff2ae49088a22195c4ae6bcde
    56 N12827911bd434e7581a9ab23a5e8fcbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    57 schema:name Cell Line
    58 rdf:type schema:DefinedTerm
    59 N12e63b3126544368b29128703a45f9c1 rdf:first sg:person.01016530662.81
    60 rdf:rest Nad8a5237f4744e3580610ae47c5f07c5
    61 N143f78a703dd4b2d9cbf7bd213eb1cb8 schema:name Springer Nature - SN SciGraph project
    62 rdf:type schema:Organization
    63 N15201614a5fb4f4bbf16bda4947c496c schema:name pubmed_id
    64 schema:value 16862139
    65 rdf:type schema:PropertyValue
    66 N15ea027a85bd462d962a7bf17aef4ff4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Cell Survival
    68 rdf:type schema:DefinedTerm
    69 N2883d02f301e4dd9b9d1026bcea2f6f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name Tissue Engineering
    71 rdf:type schema:DefinedTerm
    72 N2ed9cbdee2f9491e8ace47e988712cf6 schema:name doi
    73 schema:value 10.1038/nmeth902
    74 rdf:type schema:PropertyValue
    75 N310ba73eafd94bb9b16b1c3a3892444c schema:volumeNumber 3
    76 rdf:type schema:PublicationVolume
    77 N40c8e312ac6c4d04862ebf58f05a8def schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Humans
    79 rdf:type schema:DefinedTerm
    80 N486ea65a3c2d46c49c5b6bee757ded96 schema:name dimensions_id
    81 schema:value pub.1019814710
    82 rdf:type schema:PropertyValue
    83 N4f49cb7736124d9c8079519ebd12434d schema:name readcube_id
    84 schema:value 5e688f1955b36e75e488b323d480fcd442c88f86e3742aff197b5e158a22edce
    85 rdf:type schema:PropertyValue
    86 N74e2fe6ff2ae49088a22195c4ae6bcde rdf:first sg:person.012266000337.10
    87 rdf:rest rdf:nil
    88 N8ef6a815cd604f8f8b218f217aefdcd6 schema:name nlm_unique_id
    89 schema:value 101215604
    90 rdf:type schema:PropertyValue
    91 N96f310be265e4019b5923c170fd29ac1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Culture Media
    93 rdf:type schema:DefinedTerm
    94 N9b2da03a2ed7497a9c22a510512efc77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Stem Cells
    96 rdf:type schema:DefinedTerm
    97 Na3e2db6a9d0b44ee87fbbd577001cf33 rdf:first sg:person.01336707071.84
    98 rdf:rest N0d0dbbce45114ffcba49bc4d3645ee33
    99 Nad8a5237f4744e3580610ae47c5f07c5 rdf:first sg:person.01145062633.75
    100 rdf:rest Na3e2db6a9d0b44ee87fbbd577001cf33
    101 Nc536ba7f4bb0468aad3b9af186046f12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Cell Proliferation
    103 rdf:type schema:DefinedTerm
    104 Nc6526077dcc54cd0874b4afdffd07b68 rdf:first sg:person.01154664726.94
    105 rdf:rest N12e63b3126544368b29128703a45f9c1
    106 Nd10c9b6a9e3248b6a9977d0051452851 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Cell Differentiation
    108 rdf:type schema:DefinedTerm
    109 Ne64c6a53f16a4f129310c7bbbdbc8a29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Cell Culture Techniques
    111 rdf:type schema:DefinedTerm
    112 Nee299b4a109a4b00a4035ed0e889fcd3 schema:issueNumber 8
    113 rdf:type schema:PublicationIssue
    114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    115 schema:name Biological Sciences
    116 rdf:type schema:DefinedTerm
    117 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    118 schema:name Biochemistry and Cell Biology
    119 rdf:type schema:DefinedTerm
    120 sg:grant.2437849 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth902
    121 rdf:type schema:MonetaryGrant
    122 sg:grant.2622167 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth902
    123 rdf:type schema:MonetaryGrant
    124 sg:journal.1033763 schema:issn 1548-7091
    125 1548-7105
    126 schema:name Nature Methods
    127 rdf:type schema:Periodical
    128 sg:person.01016530662.81 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
    129 schema:familyName Bergendahl
    130 schema:givenName Veit
    131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016530662.81
    132 rdf:type schema:Person
    133 sg:person.01056475774.13 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
    134 schema:familyName Probasco
    135 schema:givenName Mitchell D
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056475774.13
    137 rdf:type schema:Person
    138 sg:person.01145062633.75 schema:affiliation https://www.grid.ac/institutes/grid.439113.d
    139 schema:familyName Levenstein
    140 schema:givenName Mark E
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145062633.75
    142 rdf:type schema:Person
    143 sg:person.01154664726.94 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
    144 schema:familyName Ludwig
    145 schema:givenName Tenneille E
    146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154664726.94
    147 rdf:type schema:Person
    148 sg:person.012266000337.10 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
    149 schema:familyName Thomson
    150 schema:givenName James A
    151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012266000337.10
    152 rdf:type schema:Person
    153 sg:person.01336707071.84 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
    154 schema:familyName Yu
    155 schema:givenName Junying
    156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336707071.84
    157 rdf:type schema:Person
    158 sg:pub.10.1007/bf02631084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039710839
    159 https://doi.org/10.1007/bf02631084
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1038/nbt1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030407065
    162 https://doi.org/10.1038/nbt1177
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1038/nbt1189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021378655
    165 https://doi.org/10.1038/nbt1189
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1038/nbt1197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037634240
    168 https://doi.org/10.1038/nbt1197
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1038/nbt922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005956847
    171 https://doi.org/10.1038/nbt922
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1038/nmeth744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029326356
    174 https://doi.org/10.1038/nmeth744
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/0021-9673(96)00286-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049184527
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1016/j.ydbio.2004.08.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001805072
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1080/01483918308064922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002094798
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1095/biolreprod.103.021147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007519570
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1182/blood-2004-10-4065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011381855
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1242/dev.01706 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033196590
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1634/stemcells.2004-0211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045135667
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1634/stemcells.2004-0279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008652652
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1634/stemcells.2005-0247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036210003
    195 rdf:type schema:CreativeWork
    196 https://www.grid.ac/institutes/grid.14003.36 schema:alternateName University of Wisconsin–Madison
    197 schema:name Department of Anatomy, University of Wisconsin Medical School, 470 N. Charter Street, Madison, Wisconsin 53706, USA.
    198 Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA.
    199 National Primate Research Center, University of Wisconsin Graduate School, 1220 Capitol Court, Madison, Wisconsin 53705, USA.
    200 WiCell Research Institute, P.O. Box 7365, Madison, Wisconsin 53707, USA.
    201 rdf:type schema:Organization
    202 https://www.grid.ac/institutes/grid.439113.d schema:alternateName WiCell
    203 schema:name WiCell Research Institute, P.O. Box 7365, Madison, Wisconsin 53707, USA.
    204 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...