Deep tissue two-photon microscopy View Full Text


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Article Info

DATE

2005-11-18

AUTHORS

Fritjof Helmchen, Winfried Denk

ABSTRACT

With few exceptions biological tissues strongly scatter light, making high-resolution deep imaging impossible for traditional—including confocal—fluorescence microscopy. Nonlinear optical microscopy, in particular two photon–excited fluorescence microscopy, has overcome this limitation, providing large depth penetration mainly because even multiply scattered signal photons can be assigned to their origin as the result of localized nonlinear signal generation. Two-photon microscopy thus allows cellular imaging several hundred microns deep in various organs of living animals. Here we review fundamental concepts of nonlinear microscopy and discuss conditions relevant for achieving large imaging depths in intact tissue. More... »

PAGES

932-940

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  • Journal

    TITLE

    Nature Methods

    ISSUE

    12

    VOLUME

    2

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth818

    DOI

    http://dx.doi.org/10.1038/nmeth818

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028724631

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16299478


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