Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-07

AUTHORS

Tobias Cornvik, Sue-Li Dahlroth, Audur Magnusdottir, Maria Dolores Herman, Rosemarie Knaust, Monica Ekberg, Pär Nordlund

ABSTRACT

The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble protein from inclusion bodies by a filtration step at the colony level. The colony filtration (CoFi) blot is very well suited to screen libraries, and in the present work we used it to screen a deletion mutagenesis library. More... »

PAGES

507-509

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth767

DOI

http://dx.doi.org/10.1038/nmeth767

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014828308

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15973420


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