Direct selection of human genomic loci by microarray hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11

AUTHORS

Thomas J Albert, Michael N Molla, Donna M Muzny, Lynne Nazareth, David Wheeler, Xingzhi Song, Todd A Richmond, Chris M Middle, Matthew J Rodesch, Charles J Packard, George M Weinstock, Richard A Gibbs

ABSTRACT

We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions. More... »

PAGES

903

References to SciGraph publications

Journal

TITLE

Nature Methods

ISSUE

11

VOLUME

4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth1111

DOI

http://dx.doi.org/10.1038/nmeth1111

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025042325

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17934467


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