Next-generation sequencing library preparation: simultaneous fragmentation and tagging using <i>in vitro</i> transposition View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-11

ABSTRACT

The advent of next-generation sequencing has made possible genome analysis at previously unattainable depth. Roche, Illumina and Life Technologies, among others, have developed well-established platforms for deep sequencing. Regardless of the instrument, one of the bottlenecks for next-generation sequencing is the amount of time and resources required for template and library preparation. Here we describe Epicentre's Nextera™ technology (covered by issued and/or pending patents), which counters this bottleneck and simplifies the sample preparation procedure. More... »

PAGES

856

Journal

TITLE

Nature Methods

ISSUE

N/A

VOLUME

6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.f.272

DOI

http://dx.doi.org/10.1038/nmeth.f.272

DIMENSIONS

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