Protein production and purification View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-02

AUTHORS

Structural Genomics Consortium, Susanne Gräslund, Pär Nordlund, Johan Weigelt, B Martin Hallberg, James Bray, Opher Gileadi, Stefan Knapp, Udo Oppermann, Cheryl Arrowsmith, Raymond Hui, Jinrong Ming, Sirano dhe-Paganon, Hee-won Park, Alexei Savchenko, Adelinda Yee, Aled Edwards, Architecture et Fonction des Macromolécules Biologiques, Renaud Vincentelli, Christian Cambillau, Berkeley Structural Genomics Center, Rosalind Kim, Sung-Hou Kim, China Structural Genomics Consortium, Zihe Rao, Yunyu Shi, Integrated Center for Structure and Function Innovation, Thomas C Terwilliger, Chang-Yub Kim, Li-Wei Hung, Geoffrey S Waldo, Israel Structural Proteomics Center, Yoav Peleg, Shira Albeck, Tamar Unger, Orly Dym, Jaime Prilusky, Joel L Sussman, Joint Center for Structural Genomics, Ray C Stevens, Scott A Lesley, Ian A Wilson, Midwest Center for Structural Genomics, Andrzej Joachimiak, Frank Collart, Irina Dementieva, Mark I Donnelly, William H Eschenfeldt, Youngchang Kim, Lucy Stols, Ruying Wu, Min Zhou, New York Structural GenomiX Research Center for Structural Genomics, Stephen K Burley, J Spencer Emtage, J Michael Sauder, Devon Thompson, Kevin Bain, John Luz, Tarun Gheyi, Fred Zhang, Shane Atwell, Steven C Almo, Jeffrey B Bonanno, Andras Fiser, Sivasubramanian Swaminathan, F William Studier, Mark R Chance, Andrej Sali, Northeast Structural Genomics Consortium, Thomas B Acton, Rong Xiao, Li Zhao, Li Chung, John F Hunt, Liang Tong, Kellie Cunningham, Masayori Inouye, Stephen Anderson, Heleema Janjua, Ritu Shastry, Chi Kent Ho, Dongyan Wang, Huang Wang, Mei Jiang, Gaetano T Montelione, Oxford Protein Production Facility, David I Stuart, Raymond J Owens, Susan Daenke, Protein Sample Production Facility Max Delbrück Center for Molecular Medicine, Anja Schütz, Udo Heinemann, RIKEN Structural Genomics/Proteomics Initiative, Shigeyuki Yokoyama, SPINE2-Complexes, Konrad Büssow, Kristin C Gunsalus

ABSTRACT

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators. More... »

PAGES

135

References to SciGraph publications

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  • Journal

    TITLE

    Nature Methods

    ISSUE

    2

    VOLUME

    5

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.f.202

    DOI

    http://dx.doi.org/10.1038/nmeth.f.202

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035202423

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18235434


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