An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-10

AUTHORS

M Ryan Corces, Alexandro E Trevino, Emily G Hamilton, Peyton G Greenside, Nicholas A Sinnott-Armstrong, Sam Vesuna, Ansuman T Satpathy, Adam J Rubin, Kathleen S Montine, Beijing Wu, Arwa Kathiria, Seung Woo Cho, Maxwell R Mumbach, Ava C Carter, Maya Kasowski, Lisa A Orloff, Viviana I Risca, Anshul Kundaje, Paul A Khavari, Thomas J Montine, William J Greenleaf, Howard Y Chang

ABSTRACT

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies. More... »

PAGES

959-962

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.4396

DOI

http://dx.doi.org/10.1038/nmeth.4396

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091372842

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28846090


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