CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-07

AUTHORS

Cem Kuscu, Mahmut Parlak, Turan Tufan, Jiekun Yang, Karol Szlachta, Xiaolong Wei, Rashad Mammadov, Mazhar Adli

ABSTRACT

CRISPR-Cas9-induced DNA damage may have deleterious effects at high-copy-number genomic regions. Here, we use CRISPR base editors to knock out genes by changing single nucleotides to create stop codons. We show that the CRISPR-STOP method is an efficient and less deleterious alternative to wild-type Cas9 for gene-knockout studies. Early stop codons can be introduced in ∼17,000 human genes. CRISPR-STOP-mediated targeted screening demonstrates comparable efficiency to WT Cas9, which indicates the suitability of our approach for genome-wide functional screenings. More... »

PAGES

710-712

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.4327

DOI

http://dx.doi.org/10.1038/nmeth.4327

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085864346

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28581493


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