Expansion microscopy with conventional antibodies and fluorescent proteins View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04-11

AUTHORS

Tyler J Chozinski, Aaron R Halpern, Haruhisa Okawa, Hyeon-Jin Kim, Grant J Tremel, Rachel O L Wong, Joshua C Vaughan

ABSTRACT

Improved sample preparation methods allow super-resolution imaging by expansion microscopy using endogenous fluorescent protein signal and conventional fluorescently labeled antibodies.

PAGES

485-488

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.3833

DOI

http://dx.doi.org/10.1038/nmeth.3833

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019771595

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27064647


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Image Enhancement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reproducibility of Results", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sensitivity and Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Staining and Labeling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Chemistry, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chozinski", 
        "givenName": "Tyler J", 
        "id": "sg:person.01150372264.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150372264.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Chemistry, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Halpern", 
        "givenName": "Aaron R", 
        "id": "sg:person.01047557327.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047557327.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biological Structure, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okawa", 
        "givenName": "Haruhisa", 
        "id": "sg:person.01102505254.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102505254.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Chemistry, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Hyeon-Jin", 
        "id": "sg:person.0625057327.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625057327.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Chemistry, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tremel", 
        "givenName": "Grant J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biological Structure, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wong", 
        "givenName": "Rachel O L", 
        "id": "sg:person.012033226162.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012033226162.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA", 
          "id": "http://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Chemistry, University of Washington, Seattle, Washington, USA", 
            "Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vaughan", 
        "givenName": "Joshua C", 
        "id": "sg:person.013400171437.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013400171437.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nmeth.1768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043491552", 
          "https://doi.org/10.1038/nmeth.1768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030075964", 
          "https://doi.org/10.1038/nature12107"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-04-11", 
    "datePublishedReg": "2016-04-11", 
    "description": "Improved sample preparation methods allow super-resolution imaging by expansion microscopy using endogenous fluorescent protein signal and conventional fluorescently labeled antibodies.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nmeth.3833", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3146151", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2506237", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2507452", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1033763", 
        "issn": [
          "1548-7091", 
          "1548-7105"
        ], 
        "name": "Nature Methods", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "keywords": [
      "expansion microscopy", 
      "super-resolution imaging", 
      "conventional antibodies", 
      "fluorescent protein signals", 
      "preparation method", 
      "sample preparation method", 
      "microscopy", 
      "fluorescent protein", 
      "protein signals", 
      "signals", 
      "antibodies", 
      "imaging", 
      "method", 
      "protein"
    ], 
    "name": "Expansion microscopy with conventional antibodies and fluorescent proteins", 
    "pagination": "485-488", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019771595"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nmeth.3833"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27064647"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nmeth.3833", 
      "https://app.dimensions.ai/details/publication/pub.1019771595"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_712.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nmeth.3833"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3833'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3833'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3833'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3833'


 

This table displays all metadata directly associated to this object as RDF triples.

191 TRIPLES      22 PREDICATES      56 URIs      45 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nmeth.3833 schema:about N0b9d93907ead4338ae060eae354af5d0
2 N0dd630b8ddac4095b1f0b214c6fe3d35
3 N138cf578d39041a58eac59514b7270f7
4 N52bda3de3c934c82bad392054e9edb86
5 N5465a9cd583b4e6eb47a3bc15fd6c529
6 N59fdb8b6ead34f3daf5f8f7eb3d961cd
7 N5c05523ddcca4204bd1b9ed35ddea965
8 N5d0154b12a964365852ce3f065be8b35
9 N5ff4583e5d2e40f9858481b9e35a2968
10 N7396ca064a664ca680417d69f6071e03
11 Nb59da16c8a994c6b97b6ffefcf8ce613
12 Nc0cdbf69977944b8a0d9396bb0ed56d9
13 Nc5ddb594bb084f7b90a1336a91830e46
14 anzsrc-for:06
15 anzsrc-for:10
16 anzsrc-for:11
17 schema:author N4544f9566a7b48dc98f9c7d54691c4c4
18 schema:citation sg:pub.10.1038/nature12107
19 sg:pub.10.1038/nmeth.1768
20 schema:datePublished 2016-04-11
21 schema:datePublishedReg 2016-04-11
22 schema:description Improved sample preparation methods allow super-resolution imaging by expansion microscopy using endogenous fluorescent protein signal and conventional fluorescently labeled antibodies.
23 schema:genre article
24 schema:inLanguage en
25 schema:isAccessibleForFree true
26 schema:isPartOf N5e5c1eda0fbf417ea68ddfa9a5d7edbe
27 N6676af28fd874acbb3f074ec6c14397e
28 sg:journal.1033763
29 schema:keywords antibodies
30 conventional antibodies
31 expansion microscopy
32 fluorescent protein
33 fluorescent protein signals
34 imaging
35 method
36 microscopy
37 preparation method
38 protein
39 protein signals
40 sample preparation method
41 signals
42 super-resolution imaging
43 schema:name Expansion microscopy with conventional antibodies and fluorescent proteins
44 schema:pagination 485-488
45 schema:productId N3436d595ea7f47c8be20ca0531c68d70
46 N6f7a552e9b504b5d9769539e75de9f57
47 Nb2966984aeca4fd585d64044d832d009
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019771595
49 https://doi.org/10.1038/nmeth.3833
50 schema:sdDatePublished 2022-06-01T22:15
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher Nd9fc56c018a843319139867d82beb3c7
53 schema:url https://doi.org/10.1038/nmeth.3833
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N041b236b84de4256abb336004101c9c8 rdf:first sg:person.01047557327.05
58 rdf:rest Neafca330316741c08cc700eb6fab1f63
59 N0b2f7fb4d488473f9449fc3bef1baef6 rdf:first N35f867ac40d1458899dd87636e97888b
60 rdf:rest Na1cd455878174e21987e93924fa96a26
61 N0b9d93907ead4338ae060eae354af5d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Staining and Labeling
63 rdf:type schema:DefinedTerm
64 N0dd630b8ddac4095b1f0b214c6fe3d35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Mice, Inbred C57BL
66 rdf:type schema:DefinedTerm
67 N138cf578d39041a58eac59514b7270f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Microscopy, Fluorescence
69 rdf:type schema:DefinedTerm
70 N2973d205161f4256ae7be799041fddd4 rdf:first sg:person.0625057327.60
71 rdf:rest N0b2f7fb4d488473f9449fc3bef1baef6
72 N3436d595ea7f47c8be20ca0531c68d70 schema:name dimensions_id
73 schema:value pub.1019771595
74 rdf:type schema:PropertyValue
75 N35f867ac40d1458899dd87636e97888b schema:affiliation grid-institutes:grid.34477.33
76 schema:familyName Tremel
77 schema:givenName Grant J
78 rdf:type schema:Person
79 N45072d697c9d45408139ec6f0f73f12c rdf:first sg:person.013400171437.05
80 rdf:rest rdf:nil
81 N4544f9566a7b48dc98f9c7d54691c4c4 rdf:first sg:person.01150372264.24
82 rdf:rest N041b236b84de4256abb336004101c9c8
83 N52bda3de3c934c82bad392054e9edb86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Brain
85 rdf:type schema:DefinedTerm
86 N5465a9cd583b4e6eb47a3bc15fd6c529 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Microscopy, Confocal
88 rdf:type schema:DefinedTerm
89 N59fdb8b6ead34f3daf5f8f7eb3d961cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Animals
91 rdf:type schema:DefinedTerm
92 N5c05523ddcca4204bd1b9ed35ddea965 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Cell Line
94 rdf:type schema:DefinedTerm
95 N5d0154b12a964365852ce3f065be8b35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Image Enhancement
97 rdf:type schema:DefinedTerm
98 N5e5c1eda0fbf417ea68ddfa9a5d7edbe schema:issueNumber 6
99 rdf:type schema:PublicationIssue
100 N5ff4583e5d2e40f9858481b9e35a2968 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Sensitivity and Specificity
102 rdf:type schema:DefinedTerm
103 N6676af28fd874acbb3f074ec6c14397e schema:volumeNumber 13
104 rdf:type schema:PublicationVolume
105 N6f7a552e9b504b5d9769539e75de9f57 schema:name pubmed_id
106 schema:value 27064647
107 rdf:type schema:PropertyValue
108 N7396ca064a664ca680417d69f6071e03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Reproducibility of Results
110 rdf:type schema:DefinedTerm
111 Na1cd455878174e21987e93924fa96a26 rdf:first sg:person.012033226162.55
112 rdf:rest N45072d697c9d45408139ec6f0f73f12c
113 Nb2966984aeca4fd585d64044d832d009 schema:name doi
114 schema:value 10.1038/nmeth.3833
115 rdf:type schema:PropertyValue
116 Nb59da16c8a994c6b97b6ffefcf8ce613 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Transfection
118 rdf:type schema:DefinedTerm
119 Nc0cdbf69977944b8a0d9396bb0ed56d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Antibodies, Monoclonal
121 rdf:type schema:DefinedTerm
122 Nc5ddb594bb084f7b90a1336a91830e46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Luminescent Proteins
124 rdf:type schema:DefinedTerm
125 Nd9fc56c018a843319139867d82beb3c7 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 Neafca330316741c08cc700eb6fab1f63 rdf:first sg:person.01102505254.90
128 rdf:rest N2973d205161f4256ae7be799041fddd4
129 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
130 schema:name Biological Sciences
131 rdf:type schema:DefinedTerm
132 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
133 schema:name Technology
134 rdf:type schema:DefinedTerm
135 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
136 schema:name Medical and Health Sciences
137 rdf:type schema:DefinedTerm
138 sg:grant.2506237 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3833
139 rdf:type schema:MonetaryGrant
140 sg:grant.2507452 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3833
141 rdf:type schema:MonetaryGrant
142 sg:grant.3146151 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3833
143 rdf:type schema:MonetaryGrant
144 sg:journal.1033763 schema:issn 1548-7091
145 1548-7105
146 schema:name Nature Methods
147 schema:publisher Springer Nature
148 rdf:type schema:Periodical
149 sg:person.01047557327.05 schema:affiliation grid-institutes:grid.34477.33
150 schema:familyName Halpern
151 schema:givenName Aaron R
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047557327.05
153 rdf:type schema:Person
154 sg:person.01102505254.90 schema:affiliation grid-institutes:grid.34477.33
155 schema:familyName Okawa
156 schema:givenName Haruhisa
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102505254.90
158 rdf:type schema:Person
159 sg:person.01150372264.24 schema:affiliation grid-institutes:grid.34477.33
160 schema:familyName Chozinski
161 schema:givenName Tyler J
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150372264.24
163 rdf:type schema:Person
164 sg:person.012033226162.55 schema:affiliation grid-institutes:grid.34477.33
165 schema:familyName Wong
166 schema:givenName Rachel O L
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012033226162.55
168 rdf:type schema:Person
169 sg:person.013400171437.05 schema:affiliation grid-institutes:grid.34477.33
170 schema:familyName Vaughan
171 schema:givenName Joshua C
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013400171437.05
173 rdf:type schema:Person
174 sg:person.0625057327.60 schema:affiliation grid-institutes:grid.34477.33
175 schema:familyName Kim
176 schema:givenName Hyeon-Jin
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625057327.60
178 rdf:type schema:Person
179 sg:pub.10.1038/nature12107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030075964
180 https://doi.org/10.1038/nature12107
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nmeth.1768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043491552
183 https://doi.org/10.1038/nmeth.1768
184 rdf:type schema:CreativeWork
185 grid-institutes:grid.34477.33 schema:alternateName Department of Biological Structure, University of Washington, Seattle, Washington, USA
186 Department of Chemistry, University of Washington, Seattle, Washington, USA
187 Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA
188 schema:name Department of Biological Structure, University of Washington, Seattle, Washington, USA
189 Department of Chemistry, University of Washington, Seattle, Washington, USA
190 Department of Physiology and Biophysics, University of Washington, Seattle, Washington, USA
191 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...