A saposin-lipoprotein nanoparticle system for membrane proteins View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04

AUTHORS

Jens Frauenfeld, Robin Löving, Jean-Paul Armache, Andreas F-P Sonnen, Fatma Guettou, Per Moberg, Lin Zhu, Caroline Jegerschöld, Ali Flayhan, John A G Briggs, Henrik Garoff, Christian Löw, Yifan Cheng, Pär Nordlund

ABSTRACT

A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptTSo2 by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state. More... »

PAGES

345-351

References to SciGraph publications

  • 2009-09-10. Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy in NATURE
  • 2013-08. Engineered nanostructured β-sheet peptides protect membrane proteins in NATURE METHODS
  • 2015-04. Structure of the TRPA1 ion channel suggests regulatory mechanisms in NATURE
  • 1998-06. Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody in NATURE
  • 2013-06. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM in NATURE METHODS
  • 2006-12. How many drug targets are there? in NATURE REVIEWS DRUG DISCOVERY
  • 2012-09. Prevention of overfitting in cryo-EM structure determination in NATURE METHODS
  • 2011-05. Cryo-EM structure of the ribosome–SecYE complex in the membrane environment in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2013-03. Structure–function correlations of pulmonary surfactant protein SP-B and the saposin-like family of proteins in EUROPEAN BIOPHYSICS JOURNAL
  • 2014-01. Quantifying the local resolution of cryo-EM density maps in NATURE METHODS
  • 2003-02. Lipopeptide detergents designed for the structural study of membrane proteins in NATURE BIOTECHNOLOGY
  • 2013-09. Broadly neutralizing antibodies and the search for an HIV-1 vaccine: the end of the beginning in NATURE REVIEWS IMMUNOLOGY
  • 2009-10-19. Practical Considerations of Membrane Protein Instability during Purification and Crystallisation in HETEROLOGOUS EXPRESSION OF MEMBRANE PROTEINS
  • 2009-10-19. Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro in HETEROLOGOUS EXPRESSION OF MEMBRANE PROTEINS
  • 2011-12. The development of vaccines: how the past led to the future in NATURE REVIEWS MICROBIOLOGY
  • 2012-12-02. Structure of the TatC core of the twin-arginine protein transport system in NATURE
  • 2015-01. Architecture and conformational switch mechanism of the ryanodine receptor in NATURE
  • 2014-08. Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2 in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2013-12. Structure of the TRPV1 ion channel determined by electron cryo-microscopy in NATURE
  • 2015-06. Smaller Nanodiscs are Suitable for Studying Protein Lipid Interactions by Solution NMR in THE PROTEIN JOURNAL
  • 2012-11. Broad and potent neutralization of HIV-1 by a gp41-specific human antibody in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.3801

    DOI

    http://dx.doi.org/10.1038/nmeth.3801

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004403305

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26950744


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cryoelectron Microscopy", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HIV Envelope Protein gp120", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lipids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Membrane Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nanoparticles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saposins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Symporters", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Frauenfeld", 
            "givenName": "Jens", 
            "id": "sg:person.0764416352.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764416352.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "L\u00f6ving", 
            "givenName": "Robin", 
            "id": "sg:person.01046432320.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046432320.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, San Francisco", 
              "id": "https://www.grid.ac/institutes/grid.266102.1", 
              "name": [
                "Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Armache", 
            "givenName": "Jean-Paul", 
            "id": "sg:person.01122607440.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122607440.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "European Molecular Biology Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.4709.a", 
              "name": [
                "Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.", 
                "Molecular Medicine Partnership Unit, European Molecular Biology Laboratory\u2013Universit\u00e4tsklinikum Heidelberg, Heidelberg, Germany."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sonnen", 
            "givenName": "Andreas F-P", 
            "id": "sg:person.0700550550.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700550550.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guettou", 
            "givenName": "Fatma", 
            "id": "sg:person.0716303152.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716303152.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Moberg", 
            "givenName": "Per", 
            "id": "sg:person.01325460652.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325460652.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Royal Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.5037.1", 
              "name": [
                "Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.", 
                "School of Technology and Health, Royal Institute of Technology, Novum, Huddinge, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhu", 
            "givenName": "Lin", 
            "id": "sg:person.01065257201.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065257201.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Royal Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.5037.1", 
              "name": [
                "Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.", 
                "School of Technology and Health, Royal Institute of Technology, Novum, Huddinge, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jegersch\u00f6ld", 
            "givenName": "Caroline", 
            "id": "sg:person.01035627707.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035627707.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "European Molecular Biology Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.475756.2", 
              "name": [
                "EMBL Hamburg, Hamburg, Germany."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Flayhan", 
            "givenName": "Ali", 
            "id": "sg:person.0760437005.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760437005.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "European Molecular Biology Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.4709.a", 
              "name": [
                "Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.", 
                "Molecular Medicine Partnership Unit, European Molecular Biology Laboratory\u2013Universit\u00e4tsklinikum Heidelberg, Heidelberg, Germany."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Briggs", 
            "givenName": "John A G", 
            "id": "sg:person.011327121142.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011327121142.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Garoff", 
            "givenName": "Henrik", 
            "id": "sg:person.01254467327.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254467327.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "European Molecular Biology Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.475756.2", 
              "name": [
                "Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.", 
                "EMBL Hamburg, Hamburg, Germany."
              ], 
              "type": "Organization"
            }, 
            "familyName": "L\u00f6w", 
            "givenName": "Christian", 
            "id": "sg:person.0665576361.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665576361.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, San Francisco", 
              "id": "https://www.grid.ac/institutes/grid.266102.1", 
              "name": [
                "Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA.", 
                "Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cheng", 
            "givenName": "Yifan", 
            "id": "sg:person.01105063132.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105063132.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Karolinska Institute", 
              "id": "https://www.grid.ac/institutes/grid.4714.6", 
              "name": [
                "Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nordlund", 
            "givenName": "P\u00e4r", 
            "id": "sg:person.01154546573.46", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154546573.46"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1128/jvi.00530-13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000252573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.93.26.15047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001281850"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001554254", 
              "https://doi.org/10.1038/nature14367"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11544", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003615458", 
              "https://doi.org/10.1038/nature11544"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.str.2008.02.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004384521"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja310901f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008153957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcc.20084", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008225564"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2727", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009301311", 
              "https://doi.org/10.1038/nmeth.2727"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1118125109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009536718"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0136947100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011435705"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011518474", 
              "https://doi.org/10.1038/nsmb.2026"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1007227107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012339772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1178746", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015274711"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1178746", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015274711"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrd2199", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016838186", 
              "https://doi.org/10.1038/nrd2199"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrd2199", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016838186", 
              "https://doi.org/10.1038/nrd2199"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1254990", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016850620"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jsbi.1998.4007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019308993"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-60761-344-2_14", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019470775", 
              "https://doi.org/10.1007/978-1-60761-344-2_14"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-60761-344-2_14", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019470775", 
              "https://doi.org/10.1007/978-1-60761-344-2_14"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019648254", 
              "https://doi.org/10.1038/nature08291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019648254", 
              "https://doi.org/10.1038/nature08291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0077984", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019939247"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0077984", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019939247"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0077984", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019939247"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2012.09.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019988217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.80.5.2515-2528.2006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020770404"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1115743109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021698433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.febslet.2009.10.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023924777"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560070420", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024692744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560070420", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024692744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/embor.2013.107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025689029"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.ppat.0020083", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028261017"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2004.12.045", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028732921"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2533", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031486116", 
              "https://doi.org/10.1038/nmeth.2533"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.01793-15", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032330098"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jemt.1060160407", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033104948"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature13916", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033151722", 
              "https://doi.org/10.1038/nature13916"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(03)72004-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034856067"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja810046q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037068497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja810046q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037068497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.ppat.1003184", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038211798"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/31405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038246480", 
              "https://doi.org/10.1038/31405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/31405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038246480", 
              "https://doi.org/10.1038/31405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbapap.2014.09.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040202480"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2006.05.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040267501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041024070", 
              "https://doi.org/10.1038/nmeth.2115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12822", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042852549", 
              "https://doi.org/10.1038/nature12822"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt776", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042896250", 
              "https://doi.org/10.1038/nbt776"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt776", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042896250", 
              "https://doi.org/10.1038/nbt776"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2015.08.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045219124"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00249-012-0858-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045847017", 
              "https://doi.org/10.1007/s00249-012-0858-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20050051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045866395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20050051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045866395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi201289c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046481968"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi201289c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046481968"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11683", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046924159", 
              "https://doi.org/10.1038/nature11683"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1217537110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048622149"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10930-015-9613-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048627552", 
              "https://doi.org/10.1007/s10930-015-9613-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0021339", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048974141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sbi.2010.05.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049772544"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2472", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050203089", 
              "https://doi.org/10.1038/nmeth.2472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro2668", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050293142", 
              "https://doi.org/10.1038/nrmicro2668"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nri3516", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050333354", 
              "https://doi.org/10.1038/nri3516"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2012.07.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052656941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2860", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053025527", 
              "https://doi.org/10.1038/nsmb.2860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2006.07.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053321196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2006.07.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053321196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-60761-344-2_12", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053563677", 
              "https://doi.org/10.1007/978-1-60761-344-2_12"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-60761-344-2_12", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053563677", 
              "https://doi.org/10.1007/978-1-60761-344-2_12"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8235592", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062653726"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082464955", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082467650", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/020917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085109081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/020917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085109081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/020917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085109081"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-04", 
        "datePublishedReg": "2016-04-01", 
        "description": "A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptTSo2 by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nmeth.3801", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2521393", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3774738", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3935707", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2440537", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "13"
          }
        ], 
        "name": "A saposin-lipoprotein nanoparticle system for membrane proteins", 
        "pagination": "345-351", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "14fb60127ae82bd4581ce56f648f4f13c013029e8fbca95781334a8bca0885db"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26950744"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101215604"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth.3801"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1004403305"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth.3801", 
          "https://app.dimensions.ai/details/publication/pub.1004403305"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:56", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000421.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nmeth/journal/v13/n4/full/nmeth.3801.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3801'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3801'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3801'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3801'


     

    This table displays all metadata directly associated to this object as RDF triples.

    424 TRIPLES      21 PREDICATES      99 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth.3801 schema:about N1dbaae1d9bc94d0c85b4aeacf99a93ea
    2 N3e1b911b17ee40d29f0dd145dcd483ca
    3 N48fe976b7ba04d4a8d4aff96d4337205
    4 N6997fe08a4294b6fac21e567fc56354e
    5 N6ad382f1a8cb4834be2e37dbef287333
    6 Nb8fa5838630d4a49871d541017a6732c
    7 Nc5bc5cb5ef6e4e4c9e52753fcad74cb2
    8 Ncfcd003a3cdd458e90de784ed6607932
    9 Nef770e6133294a3e9d222af210e23afa
    10 Nf39da3ad6e3e497789f2652a89ffea47
    11 anzsrc-for:06
    12 anzsrc-for:0601
    13 schema:author N9bec72cb56db4570964ddf13ab724940
    14 schema:citation sg:pub.10.1007/978-1-60761-344-2_12
    15 sg:pub.10.1007/978-1-60761-344-2_14
    16 sg:pub.10.1007/s00249-012-0858-9
    17 sg:pub.10.1007/s10930-015-9613-2
    18 sg:pub.10.1038/31405
    19 sg:pub.10.1038/nature08291
    20 sg:pub.10.1038/nature11544
    21 sg:pub.10.1038/nature11683
    22 sg:pub.10.1038/nature12822
    23 sg:pub.10.1038/nature13916
    24 sg:pub.10.1038/nature14367
    25 sg:pub.10.1038/nbt776
    26 sg:pub.10.1038/nmeth.2115
    27 sg:pub.10.1038/nmeth.2472
    28 sg:pub.10.1038/nmeth.2533
    29 sg:pub.10.1038/nmeth.2727
    30 sg:pub.10.1038/nrd2199
    31 sg:pub.10.1038/nri3516
    32 sg:pub.10.1038/nrmicro2668
    33 sg:pub.10.1038/nsmb.2026
    34 sg:pub.10.1038/nsmb.2860
    35 https://app.dimensions.ai/details/publication/pub.1082464955
    36 https://app.dimensions.ai/details/publication/pub.1082467650
    37 https://doi.org/10.1002/jcc.20084
    38 https://doi.org/10.1002/jemt.1060160407
    39 https://doi.org/10.1002/pro.5560070420
    40 https://doi.org/10.1006/jsbi.1998.4007
    41 https://doi.org/10.1016/j.bbapap.2014.09.016
    42 https://doi.org/10.1016/j.febslet.2009.10.024
    43 https://doi.org/10.1016/j.jmb.2004.12.045
    44 https://doi.org/10.1016/j.jsb.2006.05.009
    45 https://doi.org/10.1016/j.jsb.2012.07.010
    46 https://doi.org/10.1016/j.jsb.2012.09.006
    47 https://doi.org/10.1016/j.jsb.2015.08.008
    48 https://doi.org/10.1016/j.sbi.2010.05.006
    49 https://doi.org/10.1016/j.str.2008.02.016
    50 https://doi.org/10.1016/j.ymeth.2006.07.019
    51 https://doi.org/10.1016/s0076-6879(03)72004-7
    52 https://doi.org/10.1021/bi201289c
    53 https://doi.org/10.1021/ja310901f
    54 https://doi.org/10.1021/ja810046q
    55 https://doi.org/10.1038/embor.2013.107
    56 https://doi.org/10.1042/bj20050051
    57 https://doi.org/10.1073/pnas.0136947100
    58 https://doi.org/10.1073/pnas.1007227107
    59 https://doi.org/10.1073/pnas.1115743109
    60 https://doi.org/10.1073/pnas.1118125109
    61 https://doi.org/10.1073/pnas.1217537110
    62 https://doi.org/10.1073/pnas.93.26.15047
    63 https://doi.org/10.1101/020917
    64 https://doi.org/10.1126/science.1178746
    65 https://doi.org/10.1126/science.1254990
    66 https://doi.org/10.1126/science.8235592
    67 https://doi.org/10.1128/jvi.00530-13
    68 https://doi.org/10.1128/jvi.01793-15
    69 https://doi.org/10.1128/jvi.80.5.2515-2528.2006
    70 https://doi.org/10.1371/journal.pone.0021339
    71 https://doi.org/10.1371/journal.pone.0077984
    72 https://doi.org/10.1371/journal.ppat.0020083
    73 https://doi.org/10.1371/journal.ppat.1003184
    74 schema:datePublished 2016-04
    75 schema:datePublishedReg 2016-04-01
    76 schema:description A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptTSo2 by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state.
    77 schema:genre research_article
    78 schema:inLanguage en
    79 schema:isAccessibleForFree true
    80 schema:isPartOf N12f49bc8559144b3b33ca567b63328b2
    81 Nb450ccaae3434f329921a0eb45c328bf
    82 sg:journal.1033763
    83 schema:name A saposin-lipoprotein nanoparticle system for membrane proteins
    84 schema:pagination 345-351
    85 schema:productId N302387aa339a458eb2d226eeaff4b9b7
    86 N920af7a803ec4319841733e44348545e
    87 Nb7cfc73779b049f8b72b6a347d44937d
    88 Nbe5e306e832f48a7891121d0e1c887ff
    89 Nd6a8d73945f64f7ba6203203df413403
    90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004403305
    91 https://doi.org/10.1038/nmeth.3801
    92 schema:sdDatePublished 2019-04-10T18:56
    93 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    94 schema:sdPublisher N3f8b9fb092e3401aa5fa8428283aa062
    95 schema:url http://www.nature.com/nmeth/journal/v13/n4/full/nmeth.3801.html
    96 sgo:license sg:explorer/license/
    97 sgo:sdDataset articles
    98 rdf:type schema:ScholarlyArticle
    99 N12f49bc8559144b3b33ca567b63328b2 schema:issueNumber 4
    100 rdf:type schema:PublicationIssue
    101 N1994cc7e6f4847ffbbef8e6ba3bc5b69 rdf:first sg:person.01105063132.13
    102 rdf:rest N51eaeec001b44da9ad67edde601c50ea
    103 N1dbaae1d9bc94d0c85b4aeacf99a93ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Nanoparticles
    105 rdf:type schema:DefinedTerm
    106 N2177fda0a3b646c0ade2790935eff662 rdf:first sg:person.01046432320.83
    107 rdf:rest Nc518be52c6934c668dff246e36fe5105
    108 N302387aa339a458eb2d226eeaff4b9b7 schema:name dimensions_id
    109 schema:value pub.1004403305
    110 rdf:type schema:PropertyValue
    111 N3536363eae254dc28286665ee46f9bca rdf:first sg:person.0665576361.72
    112 rdf:rest N1994cc7e6f4847ffbbef8e6ba3bc5b69
    113 N3e1b911b17ee40d29f0dd145dcd483ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Cryoelectron Microscopy
    115 rdf:type schema:DefinedTerm
    116 N3f8b9fb092e3401aa5fa8428283aa062 schema:name Springer Nature - SN SciGraph project
    117 rdf:type schema:Organization
    118 N42f66135e39c47fdb0f9cb0d2fa4ccda rdf:first sg:person.01325460652.50
    119 rdf:rest Nea02194efad74a35bcbf8a93fa3ea8d0
    120 N43db36b7676a4da98cd85551f7fb1c7d rdf:first sg:person.011327121142.30
    121 rdf:rest Nedd115251035499d8d91e4e55093a0a8
    122 N43fe64921fd446338d3804d6f15fedbd rdf:first sg:person.0700550550.30
    123 rdf:rest N7089398fbf0b4a25805702c66c910c9d
    124 N48fe976b7ba04d4a8d4aff96d4337205 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Symporters
    126 rdf:type schema:DefinedTerm
    127 N51eaeec001b44da9ad67edde601c50ea rdf:first sg:person.01154546573.46
    128 rdf:rest rdf:nil
    129 N6997fe08a4294b6fac21e567fc56354e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Saposins
    131 rdf:type schema:DefinedTerm
    132 N6ad382f1a8cb4834be2e37dbef287333 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Membrane Proteins
    134 rdf:type schema:DefinedTerm
    135 N7089398fbf0b4a25805702c66c910c9d rdf:first sg:person.0716303152.26
    136 rdf:rest N42f66135e39c47fdb0f9cb0d2fa4ccda
    137 N920af7a803ec4319841733e44348545e schema:name pubmed_id
    138 schema:value 26950744
    139 rdf:type schema:PropertyValue
    140 N9bec72cb56db4570964ddf13ab724940 rdf:first sg:person.0764416352.51
    141 rdf:rest N2177fda0a3b646c0ade2790935eff662
    142 Nb01f8970348c481d8e6a3614d13325ae rdf:first sg:person.01035627707.44
    143 rdf:rest Nfed722df44a54c09a0120a2aa8e518db
    144 Nb450ccaae3434f329921a0eb45c328bf schema:volumeNumber 13
    145 rdf:type schema:PublicationVolume
    146 Nb7cfc73779b049f8b72b6a347d44937d schema:name nlm_unique_id
    147 schema:value 101215604
    148 rdf:type schema:PropertyValue
    149 Nb8fa5838630d4a49871d541017a6732c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name HIV Envelope Protein gp120
    151 rdf:type schema:DefinedTerm
    152 Nbe5e306e832f48a7891121d0e1c887ff schema:name readcube_id
    153 schema:value 14fb60127ae82bd4581ce56f648f4f13c013029e8fbca95781334a8bca0885db
    154 rdf:type schema:PropertyValue
    155 Nc518be52c6934c668dff246e36fe5105 rdf:first sg:person.01122607440.59
    156 rdf:rest N43fe64921fd446338d3804d6f15fedbd
    157 Nc5bc5cb5ef6e4e4c9e52753fcad74cb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Lipids
    159 rdf:type schema:DefinedTerm
    160 Ncfcd003a3cdd458e90de784ed6607932 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Humans
    162 rdf:type schema:DefinedTerm
    163 Nd6a8d73945f64f7ba6203203df413403 schema:name doi
    164 schema:value 10.1038/nmeth.3801
    165 rdf:type schema:PropertyValue
    166 Nea02194efad74a35bcbf8a93fa3ea8d0 rdf:first sg:person.01065257201.02
    167 rdf:rest Nb01f8970348c481d8e6a3614d13325ae
    168 Nedd115251035499d8d91e4e55093a0a8 rdf:first sg:person.01254467327.01
    169 rdf:rest N3536363eae254dc28286665ee46f9bca
    170 Nef770e6133294a3e9d222af210e23afa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Bacterial Proteins
    172 rdf:type schema:DefinedTerm
    173 Nf39da3ad6e3e497789f2652a89ffea47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Protein Conformation
    175 rdf:type schema:DefinedTerm
    176 Nfed722df44a54c09a0120a2aa8e518db rdf:first sg:person.0760437005.49
    177 rdf:rest N43db36b7676a4da98cd85551f7fb1c7d
    178 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    179 schema:name Biological Sciences
    180 rdf:type schema:DefinedTerm
    181 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biochemistry and Cell Biology
    183 rdf:type schema:DefinedTerm
    184 sg:grant.2440537 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3801
    185 rdf:type schema:MonetaryGrant
    186 sg:grant.2521393 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3801
    187 rdf:type schema:MonetaryGrant
    188 sg:grant.3774738 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3801
    189 rdf:type schema:MonetaryGrant
    190 sg:grant.3935707 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3801
    191 rdf:type schema:MonetaryGrant
    192 sg:journal.1033763 schema:issn 1548-7091
    193 1548-7105
    194 schema:name Nature Methods
    195 rdf:type schema:Periodical
    196 sg:person.01035627707.44 schema:affiliation https://www.grid.ac/institutes/grid.5037.1
    197 schema:familyName Jegerschöld
    198 schema:givenName Caroline
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035627707.44
    200 rdf:type schema:Person
    201 sg:person.01046432320.83 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    202 schema:familyName Löving
    203 schema:givenName Robin
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046432320.83
    205 rdf:type schema:Person
    206 sg:person.01065257201.02 schema:affiliation https://www.grid.ac/institutes/grid.5037.1
    207 schema:familyName Zhu
    208 schema:givenName Lin
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065257201.02
    210 rdf:type schema:Person
    211 sg:person.01105063132.13 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
    212 schema:familyName Cheng
    213 schema:givenName Yifan
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105063132.13
    215 rdf:type schema:Person
    216 sg:person.01122607440.59 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
    217 schema:familyName Armache
    218 schema:givenName Jean-Paul
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122607440.59
    220 rdf:type schema:Person
    221 sg:person.011327121142.30 schema:affiliation https://www.grid.ac/institutes/grid.4709.a
    222 schema:familyName Briggs
    223 schema:givenName John A G
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011327121142.30
    225 rdf:type schema:Person
    226 sg:person.01154546573.46 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    227 schema:familyName Nordlund
    228 schema:givenName Pär
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154546573.46
    230 rdf:type schema:Person
    231 sg:person.01254467327.01 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    232 schema:familyName Garoff
    233 schema:givenName Henrik
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254467327.01
    235 rdf:type schema:Person
    236 sg:person.01325460652.50 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    237 schema:familyName Moberg
    238 schema:givenName Per
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325460652.50
    240 rdf:type schema:Person
    241 sg:person.0665576361.72 schema:affiliation https://www.grid.ac/institutes/grid.475756.2
    242 schema:familyName Löw
    243 schema:givenName Christian
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665576361.72
    245 rdf:type schema:Person
    246 sg:person.0700550550.30 schema:affiliation https://www.grid.ac/institutes/grid.4709.a
    247 schema:familyName Sonnen
    248 schema:givenName Andreas F-P
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700550550.30
    250 rdf:type schema:Person
    251 sg:person.0716303152.26 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    252 schema:familyName Guettou
    253 schema:givenName Fatma
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716303152.26
    255 rdf:type schema:Person
    256 sg:person.0760437005.49 schema:affiliation https://www.grid.ac/institutes/grid.475756.2
    257 schema:familyName Flayhan
    258 schema:givenName Ali
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760437005.49
    260 rdf:type schema:Person
    261 sg:person.0764416352.51 schema:affiliation https://www.grid.ac/institutes/grid.4714.6
    262 schema:familyName Frauenfeld
    263 schema:givenName Jens
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764416352.51
    265 rdf:type schema:Person
    266 sg:pub.10.1007/978-1-60761-344-2_12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053563677
    267 https://doi.org/10.1007/978-1-60761-344-2_12
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1007/978-1-60761-344-2_14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019470775
    270 https://doi.org/10.1007/978-1-60761-344-2_14
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1007/s00249-012-0858-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045847017
    273 https://doi.org/10.1007/s00249-012-0858-9
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1007/s10930-015-9613-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048627552
    276 https://doi.org/10.1007/s10930-015-9613-2
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/31405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038246480
    279 https://doi.org/10.1038/31405
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1038/nature08291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019648254
    282 https://doi.org/10.1038/nature08291
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1038/nature11544 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003615458
    285 https://doi.org/10.1038/nature11544
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1038/nature11683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046924159
    288 https://doi.org/10.1038/nature11683
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1038/nature12822 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042852549
    291 https://doi.org/10.1038/nature12822
    292 rdf:type schema:CreativeWork
    293 sg:pub.10.1038/nature13916 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033151722
    294 https://doi.org/10.1038/nature13916
    295 rdf:type schema:CreativeWork
    296 sg:pub.10.1038/nature14367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001554254
    297 https://doi.org/10.1038/nature14367
    298 rdf:type schema:CreativeWork
    299 sg:pub.10.1038/nbt776 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042896250
    300 https://doi.org/10.1038/nbt776
    301 rdf:type schema:CreativeWork
    302 sg:pub.10.1038/nmeth.2115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041024070
    303 https://doi.org/10.1038/nmeth.2115
    304 rdf:type schema:CreativeWork
    305 sg:pub.10.1038/nmeth.2472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050203089
    306 https://doi.org/10.1038/nmeth.2472
    307 rdf:type schema:CreativeWork
    308 sg:pub.10.1038/nmeth.2533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031486116
    309 https://doi.org/10.1038/nmeth.2533
    310 rdf:type schema:CreativeWork
    311 sg:pub.10.1038/nmeth.2727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009301311
    312 https://doi.org/10.1038/nmeth.2727
    313 rdf:type schema:CreativeWork
    314 sg:pub.10.1038/nrd2199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016838186
    315 https://doi.org/10.1038/nrd2199
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1038/nri3516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050333354
    318 https://doi.org/10.1038/nri3516
    319 rdf:type schema:CreativeWork
    320 sg:pub.10.1038/nrmicro2668 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050293142
    321 https://doi.org/10.1038/nrmicro2668
    322 rdf:type schema:CreativeWork
    323 sg:pub.10.1038/nsmb.2026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011518474
    324 https://doi.org/10.1038/nsmb.2026
    325 rdf:type schema:CreativeWork
    326 sg:pub.10.1038/nsmb.2860 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053025527
    327 https://doi.org/10.1038/nsmb.2860
    328 rdf:type schema:CreativeWork
    329 https://app.dimensions.ai/details/publication/pub.1082464955 schema:CreativeWork
    330 https://app.dimensions.ai/details/publication/pub.1082467650 schema:CreativeWork
    331 https://doi.org/10.1002/jcc.20084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008225564
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1002/jemt.1060160407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033104948
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1002/pro.5560070420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024692744
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1006/jsbi.1998.4007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019308993
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1016/j.bbapap.2014.09.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040202480
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1016/j.febslet.2009.10.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023924777
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1016/j.jmb.2004.12.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028732921
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1016/j.jsb.2006.05.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040267501
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1016/j.jsb.2012.07.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052656941
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1016/j.jsb.2012.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019988217
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1016/j.jsb.2015.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045219124
    352 rdf:type schema:CreativeWork
    353 https://doi.org/10.1016/j.sbi.2010.05.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049772544
    354 rdf:type schema:CreativeWork
    355 https://doi.org/10.1016/j.str.2008.02.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004384521
    356 rdf:type schema:CreativeWork
    357 https://doi.org/10.1016/j.ymeth.2006.07.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053321196
    358 rdf:type schema:CreativeWork
    359 https://doi.org/10.1016/s0076-6879(03)72004-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034856067
    360 rdf:type schema:CreativeWork
    361 https://doi.org/10.1021/bi201289c schema:sameAs https://app.dimensions.ai/details/publication/pub.1046481968
    362 rdf:type schema:CreativeWork
    363 https://doi.org/10.1021/ja310901f schema:sameAs https://app.dimensions.ai/details/publication/pub.1008153957
    364 rdf:type schema:CreativeWork
    365 https://doi.org/10.1021/ja810046q schema:sameAs https://app.dimensions.ai/details/publication/pub.1037068497
    366 rdf:type schema:CreativeWork
    367 https://doi.org/10.1038/embor.2013.107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025689029
    368 rdf:type schema:CreativeWork
    369 https://doi.org/10.1042/bj20050051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045866395
    370 rdf:type schema:CreativeWork
    371 https://doi.org/10.1073/pnas.0136947100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011435705
    372 rdf:type schema:CreativeWork
    373 https://doi.org/10.1073/pnas.1007227107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012339772
    374 rdf:type schema:CreativeWork
    375 https://doi.org/10.1073/pnas.1115743109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021698433
    376 rdf:type schema:CreativeWork
    377 https://doi.org/10.1073/pnas.1118125109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009536718
    378 rdf:type schema:CreativeWork
    379 https://doi.org/10.1073/pnas.1217537110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048622149
    380 rdf:type schema:CreativeWork
    381 https://doi.org/10.1073/pnas.93.26.15047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001281850
    382 rdf:type schema:CreativeWork
    383 https://doi.org/10.1101/020917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085109081
    384 rdf:type schema:CreativeWork
    385 https://doi.org/10.1126/science.1178746 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015274711
    386 rdf:type schema:CreativeWork
    387 https://doi.org/10.1126/science.1254990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016850620
    388 rdf:type schema:CreativeWork
    389 https://doi.org/10.1126/science.8235592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062653726
    390 rdf:type schema:CreativeWork
    391 https://doi.org/10.1128/jvi.00530-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000252573
    392 rdf:type schema:CreativeWork
    393 https://doi.org/10.1128/jvi.01793-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032330098
    394 rdf:type schema:CreativeWork
    395 https://doi.org/10.1128/jvi.80.5.2515-2528.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020770404
    396 rdf:type schema:CreativeWork
    397 https://doi.org/10.1371/journal.pone.0021339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048974141
    398 rdf:type schema:CreativeWork
    399 https://doi.org/10.1371/journal.pone.0077984 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019939247
    400 rdf:type schema:CreativeWork
    401 https://doi.org/10.1371/journal.ppat.0020083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028261017
    402 rdf:type schema:CreativeWork
    403 https://doi.org/10.1371/journal.ppat.1003184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038211798
    404 rdf:type schema:CreativeWork
    405 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
    406 schema:name Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA.
    407 Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA.
    408 rdf:type schema:Organization
    409 https://www.grid.ac/institutes/grid.4709.a schema:alternateName European Molecular Biology Laboratory
    410 schema:name Molecular Medicine Partnership Unit, European Molecular Biology Laboratory–Universitätsklinikum Heidelberg, Heidelberg, Germany.
    411 Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    412 rdf:type schema:Organization
    413 https://www.grid.ac/institutes/grid.4714.6 schema:alternateName Karolinska Institute
    414 schema:name Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
    415 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
    416 rdf:type schema:Organization
    417 https://www.grid.ac/institutes/grid.475756.2 schema:alternateName European Molecular Biology Laboratory
    418 schema:name Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
    419 EMBL Hamburg, Hamburg, Germany.
    420 rdf:type schema:Organization
    421 https://www.grid.ac/institutes/grid.5037.1 schema:alternateName Royal Institute of Technology
    422 schema:name Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
    423 School of Technology and Health, Royal Institute of Technology, Novum, Huddinge, Sweden.
    424 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...