Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-09

AUTHORS

Jale Schneider, Jasmin Zahn, Marta Maglione, Stephan J Sigrist, Jonas Marquard, Jakub Chojnacki, Hans-Georg Kräusslich, Steffen J Sahl, Johann Engelhardt, Stefan W Hell

ABSTRACT

Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution. More... »

PAGES

827-830

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.3481

DOI

http://dx.doi.org/10.1038/nmeth.3481

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002790340

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26214129


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