Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-07-13

AUTHORS

Raj Chari, Prashant Mali, Mark Moosburner, George M Church

ABSTRACT

Assessing the activity of >1,000 single guide RNAs (sgRNAs) on >1,000 genetic loci with two Cas9 orthologs provides insight into sgRNA design and epigenetic parameters for optimal activity.

PAGES

823-826

References to SciGraph publications

  • 2013-08-01. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering in NATURE BIOTECHNOLOGY
  • 2013-08-11. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity in NATURE BIOTECHNOLOGY
  • 2013-06-23. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells in NATURE BIOTECHNOLOGY
  • 2014-04-20. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells in NATURE BIOTECHNOLOGY
  • 2014-12-16. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases in NATURE BIOTECHNOLOGY
  • 2014-09-03. Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation in NATURE BIOTECHNOLOGY
  • 2004-03. A census of human cancer genes in NATURE REVIEWS CANCER
  • 2014-01-26. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs in NATURE BIOTECHNOLOGY
  • 2012-09-02. Coupling endonucleases with DNA end–processing enzymes to drive gene disruption in NATURE METHODS
  • 2014-05-18. Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease in NATURE BIOTECHNOLOGY
  • 2012-09-05. An integrated encyclopedia of DNA elements in the human genome in NATURE
  • 2014-04-25. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification in NATURE BIOTECHNOLOGY
  • 2014-04-25. Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing in NATURE BIOTECHNOLOGY
  • 2013-09-29. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing in NATURE METHODS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.3473

    DOI

    http://dx.doi.org/10.1038/nmeth.3473

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051395827

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26167643


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Technology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromosome Mapping", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Clustered Regularly Interspaced Short Palindromic Repeats", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Human", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genomic Library", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Software", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chari", 
            "givenName": "Raj", 
            "id": "sg:person.01140176275.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140176275.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Bioengineering, University of California, San Diego, La Jolla, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.266100.3", 
              "name": [
                "Department of Bioengineering, University of California, San Diego, La Jolla, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mali", 
            "givenName": "Prashant", 
            "id": "sg:person.0667466715.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667466715.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Scripps Institute of Oceanography, University of California, San Diego, La Jolla, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.266100.3", 
              "name": [
                "Scripps Institute of Oceanography, University of California, San Diego, La Jolla, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Moosburner", 
            "givenName": "Mark", 
            "id": "sg:person.01120143715.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120143715.24"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA", 
                "Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Church", 
            "givenName": "George M", 
            "id": "sg:person.01115626315.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115626315.03"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nbt.2916", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043348902", 
              "https://doi.org/10.1038/nbt.2916"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3117", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021849416", 
              "https://doi.org/10.1038/nbt.3117"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2908", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012868720", 
              "https://doi.org/10.1038/nbt.2908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043519930", 
              "https://doi.org/10.1038/nbt.2889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2808", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048637524", 
              "https://doi.org/10.1038/nbt.2808"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2909", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053226125", 
              "https://doi.org/10.1038/nbt.2909"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000158215", 
              "https://doi.org/10.1038/nmeth.2177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007302590", 
              "https://doi.org/10.1038/nbt.2673"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2675", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041659759", 
              "https://doi.org/10.1038/nbt.2675"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029065430", 
              "https://doi.org/10.1038/nature11247"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1299", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022731034", 
              "https://doi.org/10.1038/nrc1299"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033528408", 
              "https://doi.org/10.1038/nbt.3026"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009511624", 
              "https://doi.org/10.1038/nbt.2623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2681", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028304735", 
              "https://doi.org/10.1038/nmeth.2681"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-07-13", 
        "datePublishedReg": "2015-07-13", 
        "description": "Assessing the activity of >1,000 single guide RNAs (sgRNAs) on >1,000 genetic loci with two Cas9 orthologs provides insight into sgRNA design and epigenetic parameters for optimal activity.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nmeth.3473", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2440618", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "9", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "keywords": [
          "single guide RNAs", 
          "Cas9 orthologs", 
          "guide RNA", 
          "genetic loci", 
          "sgRNA design", 
          "epigenetic parameters", 
          "optimal activity", 
          "library approach", 
          "orthologs", 
          "CRISPR", 
          "loci", 
          "RNA", 
          "activity", 
          "library", 
          "insights", 
          "approach", 
          "parameters", 
          "design", 
          "engineering parameters"
        ], 
        "name": "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach", 
        "pagination": "823-826", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1051395827"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth.3473"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26167643"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth.3473", 
          "https://app.dimensions.ai/details/publication/pub.1051395827"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T20:59", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_650.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nmeth.3473"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3473'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3473'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3473'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.3473'


     

    This table displays all metadata directly associated to this object as RDF triples.

    202 TRIPLES      21 PREDICATES      67 URIs      44 LITERALS      15 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth.3473 schema:about N427d66c7b85349bbba78b1143dc44aa9
    2 N542bf21f2e25495fa7799a68f83a40c8
    3 N64cf114e855746dea095da3bbbc33096
    4 N70318c02c9864438bb414aaf8eb22f73
    5 N83a0242db4dc4009b68d83cca4b61010
    6 Ncb72f320b26845b58f6abab28d64211f
    7 Ndf93c942841f4429a8b24873f388d841
    8 Ne752d05722754182b09043806c1f250d
    9 anzsrc-for:06
    10 anzsrc-for:10
    11 anzsrc-for:11
    12 schema:author N95d1429489ce4d2596a2010d4f396a29
    13 schema:citation sg:pub.10.1038/nature11247
    14 sg:pub.10.1038/nbt.2623
    15 sg:pub.10.1038/nbt.2673
    16 sg:pub.10.1038/nbt.2675
    17 sg:pub.10.1038/nbt.2808
    18 sg:pub.10.1038/nbt.2889
    19 sg:pub.10.1038/nbt.2908
    20 sg:pub.10.1038/nbt.2909
    21 sg:pub.10.1038/nbt.2916
    22 sg:pub.10.1038/nbt.3026
    23 sg:pub.10.1038/nbt.3117
    24 sg:pub.10.1038/nmeth.2177
    25 sg:pub.10.1038/nmeth.2681
    26 sg:pub.10.1038/nrc1299
    27 schema:datePublished 2015-07-13
    28 schema:datePublishedReg 2015-07-13
    29 schema:description Assessing the activity of >1,000 single guide RNAs (sgRNAs) on >1,000 genetic loci with two Cas9 orthologs provides insight into sgRNA design and epigenetic parameters for optimal activity.
    30 schema:genre article
    31 schema:isAccessibleForFree true
    32 schema:isPartOf Ncf5d4cfa2f5e4329b7da559405720ba3
    33 Nd62aa8de4fbc4fc896e898aac683cb6d
    34 sg:journal.1033763
    35 schema:keywords CRISPR
    36 Cas9 orthologs
    37 RNA
    38 activity
    39 approach
    40 design
    41 engineering parameters
    42 epigenetic parameters
    43 genetic loci
    44 guide RNA
    45 insights
    46 library
    47 library approach
    48 loci
    49 optimal activity
    50 orthologs
    51 parameters
    52 sgRNA design
    53 single guide RNAs
    54 schema:name Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
    55 schema:pagination 823-826
    56 schema:productId N10bd0761745f45639af909b82f0df43c
    57 N6742f60cc63049d084faa8210de62c46
    58 N99acb41b38c941bcb73d7ded43a53531
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051395827
    60 https://doi.org/10.1038/nmeth.3473
    61 schema:sdDatePublished 2022-11-24T20:59
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher N0ac42a86baea473384a3800916d304d4
    64 schema:url https://doi.org/10.1038/nmeth.3473
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N0ac42a86baea473384a3800916d304d4 schema:name Springer Nature - SN SciGraph project
    69 rdf:type schema:Organization
    70 N10bd0761745f45639af909b82f0df43c schema:name dimensions_id
    71 schema:value pub.1051395827
    72 rdf:type schema:PropertyValue
    73 N2306ffe326294d9397ce86f64d1e4e0b rdf:first sg:person.01115626315.03
    74 rdf:rest rdf:nil
    75 N427d66c7b85349bbba78b1143dc44aa9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name Humans
    77 rdf:type schema:DefinedTerm
    78 N542bf21f2e25495fa7799a68f83a40c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Genome, Human
    80 rdf:type schema:DefinedTerm
    81 N64cf114e855746dea095da3bbbc33096 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Genetic Engineering
    83 rdf:type schema:DefinedTerm
    84 N6742f60cc63049d084faa8210de62c46 schema:name pubmed_id
    85 schema:value 26167643
    86 rdf:type schema:PropertyValue
    87 N70318c02c9864438bb414aaf8eb22f73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Clustered Regularly Interspaced Short Palindromic Repeats
    89 rdf:type schema:DefinedTerm
    90 N79dc58721c3a4a60b6acb0311fe98a67 rdf:first sg:person.0667466715.32
    91 rdf:rest Nb70cb6250e9b46f9acff004afdfe5df1
    92 N83a0242db4dc4009b68d83cca4b61010 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Chromosome Mapping
    94 rdf:type schema:DefinedTerm
    95 N95d1429489ce4d2596a2010d4f396a29 rdf:first sg:person.01140176275.50
    96 rdf:rest N79dc58721c3a4a60b6acb0311fe98a67
    97 N99acb41b38c941bcb73d7ded43a53531 schema:name doi
    98 schema:value 10.1038/nmeth.3473
    99 rdf:type schema:PropertyValue
    100 Nb70cb6250e9b46f9acff004afdfe5df1 rdf:first sg:person.01120143715.24
    101 rdf:rest N2306ffe326294d9397ce86f64d1e4e0b
    102 Ncb72f320b26845b58f6abab28d64211f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Genomic Library
    104 rdf:type schema:DefinedTerm
    105 Ncf5d4cfa2f5e4329b7da559405720ba3 schema:issueNumber 9
    106 rdf:type schema:PublicationIssue
    107 Nd62aa8de4fbc4fc896e898aac683cb6d schema:volumeNumber 12
    108 rdf:type schema:PublicationVolume
    109 Ndf93c942841f4429a8b24873f388d841 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Software
    111 rdf:type schema:DefinedTerm
    112 Ne752d05722754182b09043806c1f250d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name RNA
    114 rdf:type schema:DefinedTerm
    115 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    116 schema:name Biological Sciences
    117 rdf:type schema:DefinedTerm
    118 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
    119 schema:name Technology
    120 rdf:type schema:DefinedTerm
    121 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Medical and Health Sciences
    123 rdf:type schema:DefinedTerm
    124 sg:grant.2440618 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.3473
    125 rdf:type schema:MonetaryGrant
    126 sg:journal.1033763 schema:issn 1548-7091
    127 1548-7105
    128 schema:name Nature Methods
    129 schema:publisher Springer Nature
    130 rdf:type schema:Periodical
    131 sg:person.01115626315.03 schema:affiliation grid-institutes:grid.38142.3c
    132 schema:familyName Church
    133 schema:givenName George M
    134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115626315.03
    135 rdf:type schema:Person
    136 sg:person.01120143715.24 schema:affiliation grid-institutes:grid.266100.3
    137 schema:familyName Moosburner
    138 schema:givenName Mark
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120143715.24
    140 rdf:type schema:Person
    141 sg:person.01140176275.50 schema:affiliation grid-institutes:grid.38142.3c
    142 schema:familyName Chari
    143 schema:givenName Raj
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140176275.50
    145 rdf:type schema:Person
    146 sg:person.0667466715.32 schema:affiliation grid-institutes:grid.266100.3
    147 schema:familyName Mali
    148 schema:givenName Prashant
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667466715.32
    150 rdf:type schema:Person
    151 sg:pub.10.1038/nature11247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029065430
    152 https://doi.org/10.1038/nature11247
    153 rdf:type schema:CreativeWork
    154 sg:pub.10.1038/nbt.2623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009511624
    155 https://doi.org/10.1038/nbt.2623
    156 rdf:type schema:CreativeWork
    157 sg:pub.10.1038/nbt.2673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007302590
    158 https://doi.org/10.1038/nbt.2673
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1038/nbt.2675 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041659759
    161 https://doi.org/10.1038/nbt.2675
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1038/nbt.2808 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048637524
    164 https://doi.org/10.1038/nbt.2808
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1038/nbt.2889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043519930
    167 https://doi.org/10.1038/nbt.2889
    168 rdf:type schema:CreativeWork
    169 sg:pub.10.1038/nbt.2908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012868720
    170 https://doi.org/10.1038/nbt.2908
    171 rdf:type schema:CreativeWork
    172 sg:pub.10.1038/nbt.2909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053226125
    173 https://doi.org/10.1038/nbt.2909
    174 rdf:type schema:CreativeWork
    175 sg:pub.10.1038/nbt.2916 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043348902
    176 https://doi.org/10.1038/nbt.2916
    177 rdf:type schema:CreativeWork
    178 sg:pub.10.1038/nbt.3026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033528408
    179 https://doi.org/10.1038/nbt.3026
    180 rdf:type schema:CreativeWork
    181 sg:pub.10.1038/nbt.3117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021849416
    182 https://doi.org/10.1038/nbt.3117
    183 rdf:type schema:CreativeWork
    184 sg:pub.10.1038/nmeth.2177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000158215
    185 https://doi.org/10.1038/nmeth.2177
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/nmeth.2681 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028304735
    188 https://doi.org/10.1038/nmeth.2681
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/nrc1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022731034
    191 https://doi.org/10.1038/nrc1299
    192 rdf:type schema:CreativeWork
    193 grid-institutes:grid.266100.3 schema:alternateName Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
    194 Scripps Institute of Oceanography, University of California, San Diego, La Jolla, California, USA
    195 schema:name Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
    196 Scripps Institute of Oceanography, University of California, San Diego, La Jolla, California, USA
    197 rdf:type schema:Organization
    198 grid-institutes:grid.38142.3c schema:alternateName Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    199 Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA
    200 schema:name Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    201 Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA
    202 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...