In vivo single-cell labeling by confined primed conversion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-07

AUTHORS

William P Dempsey, Lada Georgieva, Patrick M Helbling, Ali Y Sonay, Thai V Truong, Michel Haffner, Periklis Pantazis

ABSTRACT

Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells. More... »

PAGES

645-648

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.3405

DOI

http://dx.doi.org/10.1038/nmeth.3405

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000040422

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25984699


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