Massively parallel single-amino-acid mutagenesis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-01-05

AUTHORS

Jacob O Kitzman, Lea M Starita, Russell S Lo, Stanley Fields, Jay Shendure

ABSTRACT

A comprehensive allelic series for a protein of interest with a single mutation per cDNA template can be generated using a combination of microarray-based DNA synthesis and overlap-extension mutagenesis.

PAGES

203-206

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.3223

DOI

http://dx.doi.org/10.1038/nmeth.3223

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021642296

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25559584


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