The yeast two-hybrid assay: still finding connections after 25 years View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-11-25

AUTHORS

Marc Vidal, Stanley Fields

ABSTRACT

The idea of using hybrid proteins containing transcription factor domains to analyze protein-protein interactions was described in 1989. Over the past 25 years, this method has begun to reveal the complex protein networks that underlie cellular behavior.

PAGES

1203-1206

References to SciGraph publications

  • 1992-03. The C. elegans genome sequencing project: a beginning in NATURE
  • 1989-07. A novel genetic system to detect protein–protein interactions in NATURE
  • 1993-07. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast in NATURE
  • 2002-01. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry in NATURE
  • 2002-01. Functional organization of the yeast proteome by systematic analysis of protein complexes in NATURE
  • 2006-03-22. Global landscape of protein complexes in the yeast Saccharomyces cerevisiae in NATURE
  • 2008-12-07. An empirical framework for binary interactome mapping in NATURE METHODS
  • 1996-01. A protein linkage map of Escherichia coli bacteriophage T7 in NATURE GENETICS
  • 2014-09-21. Multiplex single-molecule interaction profiling of DNA-barcoded proteins in NATURE
  • 1992-05. The complete DNA sequence of yeast chromosome III in NATURE
  • 2009-10-25. 'Edgetic' perturbation of a C. elegans BCL2 ortholog in NATURE METHODS
  • 2001-11-09. Design and application of a cytokine-receptor-based interaction trap in NATURE CELL BIOLOGY
  • 2000-02. A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae in NATURE
  • 1997-07. Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens in NATURE GENETICS
  • 2004-06-09. Evidence for dynamically organized modularity in the yeast protein–protein interaction network in NATURE
  • 2006-01-22. Proteome survey reveals modularity of the yeast cell machinery in NATURE
  • 2005-09-28. Towards a proteome-scale map of the human protein–protein interaction network in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.3182

    DOI

    http://dx.doi.org/10.1038/nmeth.3182

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032892630

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25584376


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