Orthogonal Cas9 proteins for RNA-guided gene regulation and editing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-11

AUTHORS

Kevin M Esvelt, Prashant Mali, Jonathan L Braff, Mark Moosburner, Stephanie J Yaung, George M Church

ABSTRACT

The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas acquired immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but it can mediate only a single activity at a time within any given cell. Here we characterize a set of fully orthogonal Cas9 proteins and demonstrate their ability to mediate simultaneous and independently targeted gene regulation and editing in bacteria and in human cells. We find that Cas9 orthologs display consistent patterns in their recognition of target sequences, and we identify an unexpectedly versatile Cas9 protein from Neisseria meningitidis. We provide a basal set of orthogonal RNA-guided proteins for controlling biological systems and establish a general methodology for characterizing additional proteins. More... »

PAGES

1116-1121

Journal

TITLE

Nature Methods

ISSUE

11

VOLUME

10

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.2681

    DOI

    http://dx.doi.org/10.1038/nmeth.2681

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028304735

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24076762


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