Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-10

AUTHORS

Daniel J Dickinson, Jordan D Ward, David J Reiner, Bob Goldstein

ABSTRACT

Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms. Here we report a method to edit the C. elegans genome using the clustered, regularly interspersed, short palindromic repeats (CRISPR) RNA-guided Cas9 nuclease and homologous recombination. We demonstrate that Cas9 is able to induce DNA double-strand breaks with specificity for targeted sites and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, we generated gfp knock-ins and targeted mutations. Together our results outline a flexible methodology to produce essentially any desired modification in the C. elegans genome quickly and at low cost. This technology is an important addition to the array of genetic techniques already available in this experimentally tractable model organism. More... »

PAGES

1028-1034

References to SciGraph publications

  • 2012-02. Improved Mos1-mediated transgenesis in C. elegans in NATURE METHODS
  • 2013-08. Heritable genome editing in C. elegans via a CRISPR-Cas9 system in NATURE METHODS
  • 2011-03. Codon adaptation–based control of protein expression in C. elegans in NATURE METHODS
  • 2013-09. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells in NATURE BIOTECHNOLOGY
  • 2010-06. Targeted gene deletions in C. elegans using transposon excision in NATURE METHODS
  • 2013-03. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease in NATURE BIOTECHNOLOGY
  • 2005-03. Evolutionary optimization of fluorescent proteins for intracellular FRET in NATURE BIOTECHNOLOGY
  • 2013-03. RNA-guided editing of bacterial genomes using CRISPR-Cas systems in NATURE BIOTECHNOLOGY
  • 2012-10. Improving FRET dynamic range with bright green and red fluorescent proteins in NATURE METHODS
  • 2008-12. Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans in BMC MOLECULAR BIOLOGY
  • 2013-03. Efficient genome editing in zebrafish using a CRISPR-Cas system in NATURE BIOTECHNOLOGY
  • 1996-08. A non-muscle myosin required for embryonic polarity in Caenorhabditis elegans in NATURE
  • 2009-04. A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. elegans in NATURE
  • 2013-05. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting in CELL RESEARCH
  • 2013-04. Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos in CELL RESEARCH
  • 2008-11. Single-copy insertion of transgenes in Caenorhabditis elegans in NATURE GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.2641

    DOI

    http://dx.doi.org/10.1038/nmeth.2641

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050804010

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23995389


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Caenorhabditis elegans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Caenorhabditis elegans Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Clustered Regularly Interspaced Short Palindromic Repeats", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Breaks, Double-Stranded", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Knock-In Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Helminth", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Point Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinational DNA Repair", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ribonucleases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of North Carolina at Chapel Hill", 
              "id": "https://www.grid.ac/institutes/grid.10698.36", 
              "name": [
                "Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.", 
                "Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dickinson", 
            "givenName": "Daniel J", 
            "id": "sg:person.0630275115.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630275115.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, San Francisco", 
              "id": "https://www.grid.ac/institutes/grid.266102.1", 
              "name": [
                "Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ward", 
            "givenName": "Jordan D", 
            "id": "sg:person.01277331710.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277331710.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of North Carolina at Chapel Hill", 
              "id": "https://www.grid.ac/institutes/grid.10698.36", 
              "name": [
                "Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.", 
                "Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Reiner", 
            "givenName": "David J", 
            "id": "sg:person.0716650615.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716650615.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of North Carolina at Chapel Hill", 
              "id": "https://www.grid.ac/institutes/grid.10698.36", 
              "name": [
                "Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.", 
                "Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Goldstein", 
            "givenName": "Bob", 
            "id": "sg:person.0760016037.74", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760016037.74"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/382455a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001373975", 
              "https://doi.org/10.1038/382455a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.152710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003783977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.152710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003783977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.45", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006654084", 
              "https://doi.org/10.1038/cr.2013.45"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.45", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006654084", 
              "https://doi.org/10.1038/cr.2013.45"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0031849", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007080054"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2199-9-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007778040", 
              "https://doi.org/10.1186/1471-2199-9-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009511624", 
              "https://doi.org/10.1038/nbt.2623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.04.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013935649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2012.06.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015413889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2012.06.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015413889"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.108.089698", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017576829"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.108.089698", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017576829"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gnh033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018049791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.emboj.7601463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018146867"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(00)81186-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018942271"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1231143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019873131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2508", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019888616", 
              "https://doi.org/10.1038/nbt.2508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1066", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019889705", 
              "https://doi.org/10.1038/nbt1066"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1066", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019889705", 
              "https://doi.org/10.1038/nbt1066"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1232033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022072971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1454", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023315310", 
              "https://doi.org/10.1038/nmeth.1454"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2532", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023792803", 
              "https://doi.org/10.1038/nmeth.2532"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2501", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024988605", 
              "https://doi.org/10.1038/nbt.2501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1865", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025576107", 
              "https://doi.org/10.1038/nmeth.1865"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2012.08.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026136747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1565", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026484874", 
              "https://doi.org/10.1038/nmeth.1565"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2171", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026818764", 
              "https://doi.org/10.1038/nmeth.2171"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2008.08.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030191994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/dev.00735", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030671229"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030999992", 
              "https://doi.org/10.1038/nature07886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030999992", 
              "https://doi.org/10.1038/nature07886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20081949", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031754773"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20081949", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031754773"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.248", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031784174", 
              "https://doi.org/10.1038/ng.248"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.104.038265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032399666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.104.038265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032399666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gnh111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033629272"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038811201", 
              "https://doi.org/10.1038/cr.2013.46"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.155382", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040640975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.155382", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040640975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1225829", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041850060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2507", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041881936", 
              "https://doi.org/10.1038/nbt.2507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt135", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042813151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1196914", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044555726"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0030482", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046298457"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.17.3389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047265454"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2013.06.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051698837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1207773", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062464819"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1895/wormbook.1.101.1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1068724077"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074780081", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1991.tb04966.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077884622"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080072906", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082868582", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083083599", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2013-10", 
        "datePublishedReg": "2013-10-01", 
        "description": "Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms. Here we report a method to edit the C. elegans genome using the clustered, regularly interspersed, short palindromic repeats (CRISPR) RNA-guided Cas9 nuclease and homologous recombination. We demonstrate that Cas9 is able to induce DNA double-strand breaks with specificity for targeted sites and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, we generated gfp knock-ins and targeted mutations. Together our results outline a flexible methodology to produce essentially any desired modification in the C. elegans genome quickly and at low cost. This technology is an important addition to the array of genetic techniques already available in this experimentally tractable model organism. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nmeth.2641", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2439534", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2519828", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2682485", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2520163", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2634170", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3134982", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2466183", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "10"
          }
        ], 
        "name": "Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination", 
        "pagination": "1028-1034", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f2027a3b8cb8d74fd1b7de25a73056b5bc194a72605c3d5155b54915faabe1b9"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "23995389"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101215604"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth.2641"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1050804010"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth.2641", 
          "https://app.dimensions.ai/details/publication/pub.1050804010"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nmeth.2641"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2641'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2641'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2641'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2641'


     

    This table displays all metadata directly associated to this object as RDF triples.

    307 TRIPLES      21 PREDICATES      87 URIs      33 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth.2641 schema:about N023222be8c4a4c489b168eb5a1d58d81
    2 N267ede8d4c604ebe8a9cb1b78aeab83c
    3 N54d6f37bb266414b98ac232926faeb3c
    4 N6420d16a612d4f13b315a8fcb57415aa
    5 N67bd8c528d3b4a5f8b4c34fae0eafb12
    6 N7c085bb8f16540719702fe7683ec7c2c
    7 N8cb90b1e04eb42dc81dbbf1d3372f337
    8 N8d3f36bc15ef40b4bf93cff96a3a0cb1
    9 N94350b119ce443398082efeb9665bfd3
    10 Ndb7e4badc399456d825127cf0381715c
    11 Nea88bcf38ebf4c53a5faa321f2964bb9
    12 Nebd5921e1fd84038bee9b8967a7dff9a
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author N4588a007f53148c59af8a0aac92549a4
    16 schema:citation sg:pub.10.1038/382455a0
    17 sg:pub.10.1038/cr.2013.45
    18 sg:pub.10.1038/cr.2013.46
    19 sg:pub.10.1038/nature07886
    20 sg:pub.10.1038/nbt.2501
    21 sg:pub.10.1038/nbt.2507
    22 sg:pub.10.1038/nbt.2508
    23 sg:pub.10.1038/nbt.2623
    24 sg:pub.10.1038/nbt1066
    25 sg:pub.10.1038/ng.248
    26 sg:pub.10.1038/nmeth.1454
    27 sg:pub.10.1038/nmeth.1565
    28 sg:pub.10.1038/nmeth.1865
    29 sg:pub.10.1038/nmeth.2171
    30 sg:pub.10.1038/nmeth.2532
    31 sg:pub.10.1186/1471-2199-9-9
    32 https://app.dimensions.ai/details/publication/pub.1074780081
    33 https://app.dimensions.ai/details/publication/pub.1080072906
    34 https://app.dimensions.ai/details/publication/pub.1082868582
    35 https://app.dimensions.ai/details/publication/pub.1083083599
    36 https://doi.org/10.1002/j.1460-2075.1991.tb04966.x
    37 https://doi.org/10.1016/j.cell.2012.06.015
    38 https://doi.org/10.1016/j.cell.2012.08.001
    39 https://doi.org/10.1016/j.cell.2013.04.025
    40 https://doi.org/10.1016/j.celrep.2013.06.020
    41 https://doi.org/10.1016/j.cub.2008.08.013
    42 https://doi.org/10.1016/s0092-8674(00)81186-1
    43 https://doi.org/10.1038/sj.emboj.7601463
    44 https://doi.org/10.1042/bj20081949
    45 https://doi.org/10.1093/nar/25.17.3389
    46 https://doi.org/10.1093/nar/gkt135
    47 https://doi.org/10.1093/nar/gnh033
    48 https://doi.org/10.1093/nar/gnh111
    49 https://doi.org/10.1126/science.1196914
    50 https://doi.org/10.1126/science.1207773
    51 https://doi.org/10.1126/science.1225829
    52 https://doi.org/10.1126/science.1231143
    53 https://doi.org/10.1126/science.1232033
    54 https://doi.org/10.1242/dev.00735
    55 https://doi.org/10.1371/journal.pone.0030482
    56 https://doi.org/10.1371/journal.pone.0031849
    57 https://doi.org/10.1534/genetics.104.038265
    58 https://doi.org/10.1534/genetics.108.089698
    59 https://doi.org/10.1534/genetics.113.152710
    60 https://doi.org/10.1534/genetics.113.155382
    61 https://doi.org/10.1895/wormbook.1.101.1
    62 schema:datePublished 2013-10
    63 schema:datePublishedReg 2013-10-01
    64 schema:description Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms. Here we report a method to edit the C. elegans genome using the clustered, regularly interspersed, short palindromic repeats (CRISPR) RNA-guided Cas9 nuclease and homologous recombination. We demonstrate that Cas9 is able to induce DNA double-strand breaks with specificity for targeted sites and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, we generated gfp knock-ins and targeted mutations. Together our results outline a flexible methodology to produce essentially any desired modification in the C. elegans genome quickly and at low cost. This technology is an important addition to the array of genetic techniques already available in this experimentally tractable model organism.
    65 schema:genre research_article
    66 schema:inLanguage en
    67 schema:isAccessibleForFree true
    68 schema:isPartOf Nccd0593f93104909871d4ee225a8f653
    69 Ne1bef49bab5b4267947e67318aeac415
    70 sg:journal.1033763
    71 schema:name Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
    72 schema:pagination 1028-1034
    73 schema:productId N22e92f40e4564f8cbdd6dbff11968a98
    74 N70ad6f3ebb1c4bb2acde8ee505eecbba
    75 N82479e9089ea45719aad9729fa2ce0d9
    76 N94db79e7869e4414aa776e215e9bffe8
    77 Ne2259281185e48e5b35486f9b1d59c22
    78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050804010
    79 https://doi.org/10.1038/nmeth.2641
    80 schema:sdDatePublished 2019-04-10T21:25
    81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    82 schema:sdPublisher N6d4fc2c89f624c0c9f30d5b11a7af9a6
    83 schema:url http://www.nature.com/articles/nmeth.2641
    84 sgo:license sg:explorer/license/
    85 sgo:sdDataset articles
    86 rdf:type schema:ScholarlyArticle
    87 N023222be8c4a4c489b168eb5a1d58d81 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Animals
    89 rdf:type schema:DefinedTerm
    90 N0e048f665282486eb5d1c9d11da36786 rdf:first sg:person.01277331710.59
    91 rdf:rest N54dcd1a2ba4b436982c5f383a933b808
    92 N22e92f40e4564f8cbdd6dbff11968a98 schema:name dimensions_id
    93 schema:value pub.1050804010
    94 rdf:type schema:PropertyValue
    95 N267ede8d4c604ebe8a9cb1b78aeab83c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name DNA Breaks, Double-Stranded
    97 rdf:type schema:DefinedTerm
    98 N4588a007f53148c59af8a0aac92549a4 rdf:first sg:person.0630275115.61
    99 rdf:rest N0e048f665282486eb5d1c9d11da36786
    100 N54d6f37bb266414b98ac232926faeb3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Recombinant Fusion Proteins
    102 rdf:type schema:DefinedTerm
    103 N54dcd1a2ba4b436982c5f383a933b808 rdf:first sg:person.0716650615.03
    104 rdf:rest Nd7d9c2ff1d4b4096bd9257307d0751d5
    105 N6420d16a612d4f13b315a8fcb57415aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Genome, Helminth
    107 rdf:type schema:DefinedTerm
    108 N67bd8c528d3b4a5f8b4c34fae0eafb12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Gene Knock-In Techniques
    110 rdf:type schema:DefinedTerm
    111 N6d4fc2c89f624c0c9f30d5b11a7af9a6 schema:name Springer Nature - SN SciGraph project
    112 rdf:type schema:Organization
    113 N70ad6f3ebb1c4bb2acde8ee505eecbba schema:name pubmed_id
    114 schema:value 23995389
    115 rdf:type schema:PropertyValue
    116 N7c085bb8f16540719702fe7683ec7c2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Recombinational DNA Repair
    118 rdf:type schema:DefinedTerm
    119 N82479e9089ea45719aad9729fa2ce0d9 schema:name doi
    120 schema:value 10.1038/nmeth.2641
    121 rdf:type schema:PropertyValue
    122 N8cb90b1e04eb42dc81dbbf1d3372f337 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Ribonucleases
    124 rdf:type schema:DefinedTerm
    125 N8d3f36bc15ef40b4bf93cff96a3a0cb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Caenorhabditis elegans
    127 rdf:type schema:DefinedTerm
    128 N94350b119ce443398082efeb9665bfd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Caenorhabditis elegans Proteins
    130 rdf:type schema:DefinedTerm
    131 N94db79e7869e4414aa776e215e9bffe8 schema:name readcube_id
    132 schema:value f2027a3b8cb8d74fd1b7de25a73056b5bc194a72605c3d5155b54915faabe1b9
    133 rdf:type schema:PropertyValue
    134 Nccd0593f93104909871d4ee225a8f653 schema:issueNumber 10
    135 rdf:type schema:PublicationIssue
    136 Nd7d9c2ff1d4b4096bd9257307d0751d5 rdf:first sg:person.0760016037.74
    137 rdf:rest rdf:nil
    138 Ndb7e4badc399456d825127cf0381715c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Clustered Regularly Interspaced Short Palindromic Repeats
    140 rdf:type schema:DefinedTerm
    141 Ne1bef49bab5b4267947e67318aeac415 schema:volumeNumber 10
    142 rdf:type schema:PublicationVolume
    143 Ne2259281185e48e5b35486f9b1d59c22 schema:name nlm_unique_id
    144 schema:value 101215604
    145 rdf:type schema:PropertyValue
    146 Nea88bcf38ebf4c53a5faa321f2964bb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Protein Engineering
    148 rdf:type schema:DefinedTerm
    149 Nebd5921e1fd84038bee9b8967a7dff9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Point Mutation
    151 rdf:type schema:DefinedTerm
    152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    153 schema:name Biological Sciences
    154 rdf:type schema:DefinedTerm
    155 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    156 schema:name Genetics
    157 rdf:type schema:DefinedTerm
    158 sg:grant.2439534 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    159 rdf:type schema:MonetaryGrant
    160 sg:grant.2466183 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    161 rdf:type schema:MonetaryGrant
    162 sg:grant.2519828 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    163 rdf:type schema:MonetaryGrant
    164 sg:grant.2520163 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    165 rdf:type schema:MonetaryGrant
    166 sg:grant.2634170 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    167 rdf:type schema:MonetaryGrant
    168 sg:grant.2682485 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    169 rdf:type schema:MonetaryGrant
    170 sg:grant.3134982 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2641
    171 rdf:type schema:MonetaryGrant
    172 sg:journal.1033763 schema:issn 1548-7091
    173 1548-7105
    174 schema:name Nature Methods
    175 rdf:type schema:Periodical
    176 sg:person.01277331710.59 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
    177 schema:familyName Ward
    178 schema:givenName Jordan D
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277331710.59
    180 rdf:type schema:Person
    181 sg:person.0630275115.61 schema:affiliation https://www.grid.ac/institutes/grid.10698.36
    182 schema:familyName Dickinson
    183 schema:givenName Daniel J
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630275115.61
    185 rdf:type schema:Person
    186 sg:person.0716650615.03 schema:affiliation https://www.grid.ac/institutes/grid.10698.36
    187 schema:familyName Reiner
    188 schema:givenName David J
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716650615.03
    190 rdf:type schema:Person
    191 sg:person.0760016037.74 schema:affiliation https://www.grid.ac/institutes/grid.10698.36
    192 schema:familyName Goldstein
    193 schema:givenName Bob
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760016037.74
    195 rdf:type schema:Person
    196 sg:pub.10.1038/382455a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001373975
    197 https://doi.org/10.1038/382455a0
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1038/cr.2013.45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006654084
    200 https://doi.org/10.1038/cr.2013.45
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1038/cr.2013.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038811201
    203 https://doi.org/10.1038/cr.2013.46
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1038/nature07886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030999992
    206 https://doi.org/10.1038/nature07886
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1038/nbt.2501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024988605
    209 https://doi.org/10.1038/nbt.2501
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1038/nbt.2507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041881936
    212 https://doi.org/10.1038/nbt.2507
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1038/nbt.2508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019888616
    215 https://doi.org/10.1038/nbt.2508
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/nbt.2623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009511624
    218 https://doi.org/10.1038/nbt.2623
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/nbt1066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019889705
    221 https://doi.org/10.1038/nbt1066
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/ng.248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031784174
    224 https://doi.org/10.1038/ng.248
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1038/nmeth.1454 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023315310
    227 https://doi.org/10.1038/nmeth.1454
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/nmeth.1565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026484874
    230 https://doi.org/10.1038/nmeth.1565
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1038/nmeth.1865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025576107
    233 https://doi.org/10.1038/nmeth.1865
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/nmeth.2171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026818764
    236 https://doi.org/10.1038/nmeth.2171
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1038/nmeth.2532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023792803
    239 https://doi.org/10.1038/nmeth.2532
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1186/1471-2199-9-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007778040
    242 https://doi.org/10.1186/1471-2199-9-9
    243 rdf:type schema:CreativeWork
    244 https://app.dimensions.ai/details/publication/pub.1074780081 schema:CreativeWork
    245 https://app.dimensions.ai/details/publication/pub.1080072906 schema:CreativeWork
    246 https://app.dimensions.ai/details/publication/pub.1082868582 schema:CreativeWork
    247 https://app.dimensions.ai/details/publication/pub.1083083599 schema:CreativeWork
    248 https://doi.org/10.1002/j.1460-2075.1991.tb04966.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077884622
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1016/j.cell.2012.06.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015413889
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1016/j.cell.2012.08.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026136747
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/j.cell.2013.04.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013935649
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1016/j.celrep.2013.06.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051698837
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1016/j.cub.2008.08.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030191994
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1016/s0092-8674(00)81186-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018942271
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1038/sj.emboj.7601463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018146867
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1042/bj20081949 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031754773
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1093/nar/25.17.3389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047265454
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1093/nar/gkt135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042813151
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1093/nar/gnh033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018049791
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1093/nar/gnh111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033629272
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1126/science.1196914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044555726
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1126/science.1207773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062464819
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1126/science.1231143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019873131
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1126/science.1232033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022072971
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1242/dev.00735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030671229
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1371/journal.pone.0030482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046298457
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1371/journal.pone.0031849 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007080054
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1534/genetics.104.038265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032399666
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1534/genetics.108.089698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017576829
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1534/genetics.113.152710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003783977
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1534/genetics.113.155382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040640975
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1895/wormbook.1.101.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068724077
    299 rdf:type schema:CreativeWork
    300 https://www.grid.ac/institutes/grid.10698.36 schema:alternateName University of North Carolina at Chapel Hill
    301 schema:name Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
    302 Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
    303 Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
    304 rdf:type schema:Organization
    305 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
    306 schema:name Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA.
    307 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...