UPARSE: highly accurate OTU sequences from microbial amplicon reads View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-10

AUTHORS

Robert C Edgar

ABSTRACT

Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community. More... »

PAGES

996-998

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.2604

DOI

http://dx.doi.org/10.1038/nmeth.2604

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027743851

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23955772


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