RNA-guided gene activation by CRISPR-Cas9–based transcription factors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-10

AUTHORS

Pablo Perez-Pinera, D Dewran Kocak, Christopher M Vockley, Andrew F Adler, Ami M Kabadi, Lauren R Polstein, Pratiksha I Thakore, Katherine A Glass, David G Ousterout, Kam W Leong, Farshid Guilak, Gregory E Crawford, Timothy E Reddy, Charles A Gersbach

ABSTRACT

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation. More... »

PAGES

973-976

References to SciGraph publications

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  • Journal

    TITLE

    Nature Methods

    ISSUE

    10

    VOLUME

    10

    Related Patents

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.2600

    DOI

    http://dx.doi.org/10.1038/nmeth.2600

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024354143

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23892895


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