The transience of transient overexpression View Full Text


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Article Info

DATE

2013-07-30

AUTHORS

Toby J Gibson, Markus Seiler, Reiner A Veitia

ABSTRACT

Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels. More... »

PAGES

715-721

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.2534

    DOI

    http://dx.doi.org/10.1038/nmeth.2534

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039107611

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23900254


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