Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-07

AUTHORS

Fang Huang, Tobias M P Hartwich, Felix E Rivera-Molina, Yu Lin, Whitney C Duim, Jane J Long, Pradeep D Uchil, Jordan R Myers, Michelle A Baird, Walther Mothes, Michael W Davidson, Derek Toomre, Joerg Bewersdorf

ABSTRACT

Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras have the potential to dramatically accelerate data acquisition, enlarge the field of view and increase the effective quantum efficiency in single-molecule switching nanoscopy. However, sCMOS-intrinsic pixel-dependent readout noise substantially lowers the localization precision and introduces localization artifacts. We present algorithms that overcome these limitations and that provide unbiased, precise localization of single molecules at the theoretical limit. Using these in combination with a multi-emitter fitting algorithm, we demonstrate single-molecule localization super-resolution imaging at rates of up to 32 reconstructed images per second in fixed and living cells. More... »

PAGES

653-658

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.2488

DOI

http://dx.doi.org/10.1038/nmeth.2488

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024438788

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23708387


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

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curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2488'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2488'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2488'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2488'


 

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