Synergistic and tunable human gene activation by combinations of synthetic transcription factors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-03

AUTHORS

Pablo Perez-Pinera, David G Ousterout, Jonathan M Brunger, Alicia M Farin, Katherine A Glass, Farshid Guilak, Gregory E Crawford, Alexander J Hartemink, Charles A Gersbach

ABSTRACT

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology. More... »

PAGES

239

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.2361

DOI

http://dx.doi.org/10.1038/nmeth.2361

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009286919

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23377379


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