Coupling endonucleases with DNA end–processing enzymes to drive gene disruption View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-10

AUTHORS

Michael T Certo, Kamila S Gwiazda, Ryan Kuhar, Blythe Sather, Gabrielle Curinga, Tyler Mandt, Michelle Brault, Abigail R Lambert, Sarah K Baxter, Kyle Jacoby, Byoung Y Ryu, Hans-Peter Kiem, Agnes Gouble, Frederic Paques, David J Rawlings, Andrew M Scharenberg

ABSTRACT

Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates. More... »

PAGES

973

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.2177

DOI

http://dx.doi.org/10.1038/nmeth.2177

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000158215

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22941364


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Breaks, Double-Stranded", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA End-Joining Repair", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Repair", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endonucleases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Exodeoxyribonucleases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HEK293 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, CCR5", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA.", 
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Certo", 
        "givenName": "Michael T", 
        "id": "sg:person.0643526705.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643526705.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA.", 
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gwiazda", 
        "givenName": "Kamila S", 
        "id": "sg:person.0711642105.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711642105.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kuhar", 
        "givenName": "Ryan", 
        "id": "sg:person.01215764405.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215764405.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sather", 
        "givenName": "Blythe", 
        "id": "sg:person.01161316417.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161316417.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Curinga", 
        "givenName": "Gabrielle", 
        "id": "sg:person.0605361270.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605361270.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mandt", 
        "givenName": "Tyler", 
        "id": "sg:person.01142317105.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142317105.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brault", 
        "givenName": "Michelle", 
        "id": "sg:person.01210432305.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210432305.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lambert", 
        "givenName": "Abigail R", 
        "id": "sg:person.0653561057.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653561057.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA.", 
            "Department of Immunology, University of Washington, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baxter", 
        "givenName": "Sarah K", 
        "id": "sg:person.01101536005.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101536005.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA.", 
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jacoby", 
        "givenName": "Kyle", 
        "id": "sg:person.0717547255.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717547255.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Seattle Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ryu", 
        "givenName": "Byoung Y", 
        "id": "sg:person.0650276532.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650276532.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.", 
            "Department of Medicine, University of Washington, Seattle, Washington, USA.", 
            "Department of Pathology, University of Washington, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kiem", 
        "givenName": "Hans-Peter", 
        "id": "sg:person.01350411276.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350411276.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takara (France)", 
          "id": "https://www.grid.ac/institutes/grid.433267.7", 
          "name": [
            "Cellectis, Paris, France."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gouble", 
        "givenName": "Agnes", 
        "id": "sg:person.0623671433.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623671433.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Takara (France)", 
          "id": "https://www.grid.ac/institutes/grid.433267.7", 
          "name": [
            "Cellectis, Paris, France."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paques", 
        "givenName": "Frederic", 
        "id": "sg:person.01044314270.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044314270.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA.", 
            "Department of Immunology, University of Washington, Seattle, Washington, USA.", 
            "Department of Pediatrics, University of Washington, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rawlings", 
        "givenName": "David J", 
        "id": "sg:person.01127475651.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127475651.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA.", 
            "Department of Immunology, University of Washington, Seattle, Washington, USA.", 
            "Cellectis, Paris, France.", 
            "Department of Pediatrics, University of Washington, Seattle, Washington, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scharenberg", 
        "givenName": "Andrew M", 
        "id": "sg:person.01034214461.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034214461.84"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/cr.2007.111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000477639", 
          "https://doi.org/10.1038/cr.2007.111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016004947", 
          "https://doi.org/10.1038/nbt.1755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkr218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018432175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg2842", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018939216", 
          "https://doi.org/10.1038/nrg2842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg2842", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018939216", 
          "https://doi.org/10.1038/nrg2842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.052308.093131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022097335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mrfmmm.2011.02.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029936569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mt.2010.259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033087987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2009.98", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035094532", 
          "https://doi.org/10.1038/nprot.2009.98"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2009.98", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035094532", 
          "https://doi.org/10.1038/nprot.2009.98"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1648", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035945498", 
          "https://doi.org/10.1038/nmeth.1648"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0b013e32834122d7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039508480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0b013e32834122d7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039508480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/coh.0b013e32834122d7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039508480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1000683", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039576536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tig.2008.08.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040738692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1670", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043918422", 
          "https://doi.org/10.1038/nmeth.1670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl720", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046669585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14712598.5.5.627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067589633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/14712598.5.5.627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067589633"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-10", 
    "datePublishedReg": "2012-10-01", 
    "description": "Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nmeth.2177", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2441316", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2705129", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2669718", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2669747", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2683719", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2539691", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2705107", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2695894", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2669746", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2637490", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1033763", 
        "issn": [
          "1548-7091", 
          "1548-7105"
        ], 
        "name": "Nature Methods", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Coupling endonucleases with DNA end\u2013processing enzymes to drive gene disruption", 
    "pagination": "973", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "00c07ff71eec31680715d5c597a75eb99b18f1f6c73f6415d185f7ec637150e1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22941364"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101215604"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nmeth.2177"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1000158215"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nmeth.2177", 
      "https://app.dimensions.ai/details/publication/pub.1000158215"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000435.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nmeth.2177"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2177'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2177'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2177'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.2177'


 

This table displays all metadata directly associated to this object as RDF triples.

302 TRIPLES      21 PREDICATES      55 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nmeth.2177 schema:about N12c4714134b94e63ac8f904c214fa921
2 N19aceb29fd7744339ea4c7ebaab4784f
3 N4cfe4b34baeb4d5da1daea03781a414c
4 N6deee7454edf4c94997aa61f83368376
5 N74e2d09237f445d980d34c8b6f11ea5a
6 N7eba2611cbdc4f8ebe4d724b5b3431a3
7 N92d3bcabad60445d8ded2c5ec36b7c7a
8 N92f1b81fac7d4fcaaadf85ef4ae5ba92
9 Nad86d064633c44589a0c5bb32153959d
10 Nbca6b94f5c774d7882377b1885b3a410
11 Nfcba20209ca7437984b4ae2effe30681
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author N841a32e7e28547599c6aa6bf4aadd94f
15 schema:citation sg:pub.10.1038/cr.2007.111
16 sg:pub.10.1038/nbt.1755
17 sg:pub.10.1038/nmeth.1648
18 sg:pub.10.1038/nmeth.1670
19 sg:pub.10.1038/nprot.2009.98
20 sg:pub.10.1038/nrg2842
21 https://doi.org/10.1016/j.mrfmmm.2011.02.005
22 https://doi.org/10.1016/j.tig.2008.08.007
23 https://doi.org/10.1038/mt.2010.259
24 https://doi.org/10.1093/nar/gkl720
25 https://doi.org/10.1093/nar/gkr218
26 https://doi.org/10.1097/coh.0b013e32834122d7
27 https://doi.org/10.1146/annurev.biochem.052308.093131
28 https://doi.org/10.1371/journal.pgen.1000683
29 https://doi.org/10.1517/14712598.5.5.627
30 schema:datePublished 2012-10
31 schema:datePublishedReg 2012-10-01
32 schema:description Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.
33 schema:genre research_article
34 schema:inLanguage en
35 schema:isAccessibleForFree true
36 schema:isPartOf N1a7544c9d4fa4e2cab342d7168d8db97
37 N97a6922534ae443b9daebc7eadfbabc1
38 sg:journal.1033763
39 schema:name Coupling endonucleases with DNA end–processing enzymes to drive gene disruption
40 schema:pagination 973
41 schema:productId N154641f167c543f480d66db21e37a2b3
42 N1f03c186d167499583e9b5428360e3d9
43 N46638bf2a22b4dff930a1936f7eefd00
44 Nb81c0b01f1e84a738a66084579de5f44
45 Nc6c5a655becc40bf8581745cd3a3b754
46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000158215
47 https://doi.org/10.1038/nmeth.2177
48 schema:sdDatePublished 2019-04-10T15:39
49 schema:sdLicense https://scigraph.springernature.com/explorer/license/
50 schema:sdPublisher N7f052095ca164f249024182d0e5f749c
51 schema:url https://www.nature.com/articles/nmeth.2177
52 sgo:license sg:explorer/license/
53 sgo:sdDataset articles
54 rdf:type schema:ScholarlyArticle
55 N104671bfbc4b4ecb94ca8e9e86d9eccc rdf:first sg:person.01210432305.37
56 rdf:rest N2525f2d4c2424fcdbcb27c7b5a06ee35
57 N12c4714134b94e63ac8f904c214fa921 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Mice
59 rdf:type schema:DefinedTerm
60 N154641f167c543f480d66db21e37a2b3 schema:name nlm_unique_id
61 schema:value 101215604
62 rdf:type schema:PropertyValue
63 N19aceb29fd7744339ea4c7ebaab4784f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Endonucleases
65 rdf:type schema:DefinedTerm
66 N1a7544c9d4fa4e2cab342d7168d8db97 schema:issueNumber 10
67 rdf:type schema:PublicationIssue
68 N1f03c186d167499583e9b5428360e3d9 schema:name doi
69 schema:value 10.1038/nmeth.2177
70 rdf:type schema:PropertyValue
71 N2525f2d4c2424fcdbcb27c7b5a06ee35 rdf:first sg:person.0653561057.52
72 rdf:rest Nb2dcdee4b546417082ac4894378c7658
73 N2bb3bcd26c56425c9be9a1352dbbdd56 rdf:first sg:person.0650276532.33
74 rdf:rest Nab2c42456fa845ee91bbc3db4f33b85d
75 N2e0729b964134ac2b738e56b6f95272d rdf:first sg:person.01142317105.37
76 rdf:rest N104671bfbc4b4ecb94ca8e9e86d9eccc
77 N46638bf2a22b4dff930a1936f7eefd00 schema:name dimensions_id
78 schema:value pub.1000158215
79 rdf:type schema:PropertyValue
80 N4cfe4b34baeb4d5da1daea03781a414c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Animals
82 rdf:type schema:DefinedTerm
83 N624fa87dddf8486bb2c453708f3a232e rdf:first sg:person.01215764405.01
84 rdf:rest Nadb946d77c3246269a6dd5f7fc4a82d8
85 N6deee7454edf4c94997aa61f83368376 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Receptors, CCR5
87 rdf:type schema:DefinedTerm
88 N74e2d09237f445d980d34c8b6f11ea5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Exodeoxyribonucleases
90 rdf:type schema:DefinedTerm
91 N7eba2611cbdc4f8ebe4d724b5b3431a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name DNA Breaks, Double-Stranded
93 rdf:type schema:DefinedTerm
94 N7f052095ca164f249024182d0e5f749c schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 N841a32e7e28547599c6aa6bf4aadd94f rdf:first sg:person.0643526705.34
97 rdf:rest N8c22e0009e594dce9ada0393fb949b51
98 N8c22e0009e594dce9ada0393fb949b51 rdf:first sg:person.0711642105.99
99 rdf:rest N624fa87dddf8486bb2c453708f3a232e
100 N90c6e43b8f7e4327a3abe3d5a59382b2 rdf:first sg:person.0605361270.08
101 rdf:rest N2e0729b964134ac2b738e56b6f95272d
102 N9174158356654083894e0a14d1c150d5 rdf:first sg:person.01127475651.05
103 rdf:rest Nfc4f24dc4a7f483980cdfd294dbbf0c8
104 N92d3bcabad60445d8ded2c5ec36b7c7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Phosphoproteins
106 rdf:type schema:DefinedTerm
107 N92f1b81fac7d4fcaaadf85ef4ae5ba92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name DNA Repair
109 rdf:type schema:DefinedTerm
110 N97a6922534ae443b9daebc7eadfbabc1 schema:volumeNumber 9
111 rdf:type schema:PublicationVolume
112 N9cd1ec38544b4177bc92bf64579f861f rdf:first sg:person.0717547255.69
113 rdf:rest N2bb3bcd26c56425c9be9a1352dbbdd56
114 Nab2c42456fa845ee91bbc3db4f33b85d rdf:first sg:person.01350411276.47
115 rdf:rest Ndde061eb87ae49f091bce197cbec170e
116 Nad86d064633c44589a0c5bb32153959d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Humans
118 rdf:type schema:DefinedTerm
119 Nadb946d77c3246269a6dd5f7fc4a82d8 rdf:first sg:person.01161316417.13
120 rdf:rest N90c6e43b8f7e4327a3abe3d5a59382b2
121 Nb2dcdee4b546417082ac4894378c7658 rdf:first sg:person.01101536005.16
122 rdf:rest N9cd1ec38544b4177bc92bf64579f861f
123 Nb81c0b01f1e84a738a66084579de5f44 schema:name pubmed_id
124 schema:value 22941364
125 rdf:type schema:PropertyValue
126 Nbc5e3305fd4d45a18c36a54c7a0b9b1f rdf:first sg:person.01044314270.62
127 rdf:rest N9174158356654083894e0a14d1c150d5
128 Nbca6b94f5c774d7882377b1885b3a410 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name DNA End-Joining Repair
130 rdf:type schema:DefinedTerm
131 Nc6c5a655becc40bf8581745cd3a3b754 schema:name readcube_id
132 schema:value 00c07ff71eec31680715d5c597a75eb99b18f1f6c73f6415d185f7ec637150e1
133 rdf:type schema:PropertyValue
134 Ndde061eb87ae49f091bce197cbec170e rdf:first sg:person.0623671433.53
135 rdf:rest Nbc5e3305fd4d45a18c36a54c7a0b9b1f
136 Nfc4f24dc4a7f483980cdfd294dbbf0c8 rdf:first sg:person.01034214461.84
137 rdf:rest rdf:nil
138 Nfcba20209ca7437984b4ae2effe30681 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name HEK293 Cells
140 rdf:type schema:DefinedTerm
141 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biological Sciences
143 rdf:type schema:DefinedTerm
144 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
145 schema:name Genetics
146 rdf:type schema:DefinedTerm
147 sg:grant.2441316 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
148 rdf:type schema:MonetaryGrant
149 sg:grant.2539691 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
150 rdf:type schema:MonetaryGrant
151 sg:grant.2637490 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
152 rdf:type schema:MonetaryGrant
153 sg:grant.2669718 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
154 rdf:type schema:MonetaryGrant
155 sg:grant.2669746 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
156 rdf:type schema:MonetaryGrant
157 sg:grant.2669747 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
158 rdf:type schema:MonetaryGrant
159 sg:grant.2683719 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
160 rdf:type schema:MonetaryGrant
161 sg:grant.2695894 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
162 rdf:type schema:MonetaryGrant
163 sg:grant.2705107 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
164 rdf:type schema:MonetaryGrant
165 sg:grant.2705129 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.2177
166 rdf:type schema:MonetaryGrant
167 sg:journal.1033763 schema:issn 1548-7091
168 1548-7105
169 schema:name Nature Methods
170 rdf:type schema:Periodical
171 sg:person.01034214461.84 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
172 schema:familyName Scharenberg
173 schema:givenName Andrew M
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034214461.84
175 rdf:type schema:Person
176 sg:person.01044314270.62 schema:affiliation https://www.grid.ac/institutes/grid.433267.7
177 schema:familyName Paques
178 schema:givenName Frederic
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044314270.62
180 rdf:type schema:Person
181 sg:person.01101536005.16 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
182 schema:familyName Baxter
183 schema:givenName Sarah K
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101536005.16
185 rdf:type schema:Person
186 sg:person.01127475651.05 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
187 schema:familyName Rawlings
188 schema:givenName David J
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127475651.05
190 rdf:type schema:Person
191 sg:person.01142317105.37 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
192 schema:familyName Mandt
193 schema:givenName Tyler
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142317105.37
195 rdf:type schema:Person
196 sg:person.01161316417.13 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
197 schema:familyName Sather
198 schema:givenName Blythe
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161316417.13
200 rdf:type schema:Person
201 sg:person.01210432305.37 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
202 schema:familyName Brault
203 schema:givenName Michelle
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210432305.37
205 rdf:type schema:Person
206 sg:person.01215764405.01 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
207 schema:familyName Kuhar
208 schema:givenName Ryan
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215764405.01
210 rdf:type schema:Person
211 sg:person.01350411276.47 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
212 schema:familyName Kiem
213 schema:givenName Hans-Peter
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350411276.47
215 rdf:type schema:Person
216 sg:person.0605361270.08 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
217 schema:familyName Curinga
218 schema:givenName Gabrielle
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605361270.08
220 rdf:type schema:Person
221 sg:person.0623671433.53 schema:affiliation https://www.grid.ac/institutes/grid.433267.7
222 schema:familyName Gouble
223 schema:givenName Agnes
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623671433.53
225 rdf:type schema:Person
226 sg:person.0643526705.34 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
227 schema:familyName Certo
228 schema:givenName Michael T
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643526705.34
230 rdf:type schema:Person
231 sg:person.0650276532.33 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
232 schema:familyName Ryu
233 schema:givenName Byoung Y
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650276532.33
235 rdf:type schema:Person
236 sg:person.0653561057.52 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
237 schema:familyName Lambert
238 schema:givenName Abigail R
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653561057.52
240 rdf:type schema:Person
241 sg:person.0711642105.99 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
242 schema:familyName Gwiazda
243 schema:givenName Kamila S
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0711642105.99
245 rdf:type schema:Person
246 sg:person.0717547255.69 schema:affiliation https://www.grid.ac/institutes/grid.240741.4
247 schema:familyName Jacoby
248 schema:givenName Kyle
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717547255.69
250 rdf:type schema:Person
251 sg:pub.10.1038/cr.2007.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000477639
252 https://doi.org/10.1038/cr.2007.111
253 rdf:type schema:CreativeWork
254 sg:pub.10.1038/nbt.1755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016004947
255 https://doi.org/10.1038/nbt.1755
256 rdf:type schema:CreativeWork
257 sg:pub.10.1038/nmeth.1648 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035945498
258 https://doi.org/10.1038/nmeth.1648
259 rdf:type schema:CreativeWork
260 sg:pub.10.1038/nmeth.1670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043918422
261 https://doi.org/10.1038/nmeth.1670
262 rdf:type schema:CreativeWork
263 sg:pub.10.1038/nprot.2009.98 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035094532
264 https://doi.org/10.1038/nprot.2009.98
265 rdf:type schema:CreativeWork
266 sg:pub.10.1038/nrg2842 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018939216
267 https://doi.org/10.1038/nrg2842
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/j.mrfmmm.2011.02.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029936569
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/j.tig.2008.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040738692
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1038/mt.2010.259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033087987
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1093/nar/gkl720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046669585
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1093/nar/gkr218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018432175
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1097/coh.0b013e32834122d7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039508480
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1146/annurev.biochem.052308.093131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022097335
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1371/journal.pgen.1000683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039576536
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1517/14712598.5.5.627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067589633
286 rdf:type schema:CreativeWork
287 https://www.grid.ac/institutes/grid.240741.4 schema:alternateName Seattle Children's Hospital
288 schema:name Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA.
289 Molecular and Cellular Biology Program, University of Washington, Seattle, Washington, USA.
290 rdf:type schema:Organization
291 https://www.grid.ac/institutes/grid.34477.33 schema:alternateName University of Washington
292 schema:name Cellectis, Paris, France.
293 Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, Washington, USA.
294 Department of Immunology, University of Washington, Seattle, Washington, USA.
295 Department of Medicine, University of Washington, Seattle, Washington, USA.
296 Department of Pathology, University of Washington, Seattle, Washington, USA.
297 Department of Pediatrics, University of Washington, Seattle, Washington, USA.
298 Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
299 rdf:type schema:Organization
300 https://www.grid.ac/institutes/grid.433267.7 schema:alternateName Takara (France)
301 schema:name Cellectis, Paris, France.
302 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...