Multicolor two-photon tissue imaging by wavelength mixing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-07-08

AUTHORS

Pierre Mahou, Maxwell Zimmerley, Karine Loulier, Katherine S Matho, Guillaume Labroille, Xavier Morin, Willy Supatto, Jean Livet, Delphine Débarre, Emmanuel Beaurepaire

ABSTRACT

This method achieves simultaneous and spatially colocalized excitation of three fluorophores with distinct spectra, doing so via two-photon microscopy using a femtosecond laser and an optical parametric oscillator and by temporally overlapping the beams. Imaging of 'Brainbow'-labeled mouse and chicken nervous tissue and of developing fly embryos is shown.

PAGES

815-818

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.2098

DOI

http://dx.doi.org/10.1038/nmeth.2098

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006826084

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22772730


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