Bayesian localization microscopy reveals nanoscale podosome dynamics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-02

AUTHORS

Susan Cox, Edward Rosten, James Monypenny, Tijana Jovanovic-Talisman, Dylan T Burnette, Jennifer Lippincott-Schwartz, Gareth E Jones, Rainer Heintzmann

ABSTRACT

We describe a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and xenon arc lamp illumination. Our Bayesian analysis of the blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores that may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame and unifies the analysis of the localization from blinking and bleaching events. By modeling the entire dataset, we were able to use each reappearance of a fluorophore to improve the localization accuracy. The high performance of this technique allowed us to reveal the nanoscale dynamics of podosome formation and dissociation throughout an entire cell with a resolution of 50 nm on a 4-s timescale. More... »

PAGES

195

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1812

DOI

http://dx.doi.org/10.1038/nmeth.1812

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002320934

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22138825


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