High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-09

AUTHORS

Fuqiang Chen, Shondra M Pruett-Miller, Yuping Huang, Monika Gjoka, Katarzyna Duda, Jack Taunton, Trevor N Collingwood, Morten Frodin, Gregory D Davis

ABSTRACT

Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions. More... »

PAGES

753

Journal

TITLE

Nature Methods

ISSUE

9

VOLUME

8

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1653

DOI

http://dx.doi.org/10.1038/nmeth.1653

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046255083

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21765410


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