High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-07-17

AUTHORS

Fuqiang Chen, Shondra M Pruett-Miller, Yuping Huang, Monika Gjoka, Katarzyna Duda, Jack Taunton, Trevor N Collingwood, Morten Frodin, Gregory D Davis

ABSTRACT

Single-stranded oligonucleotides are used as donor templates for zinc-finger nucleases to create targeted genomic deletions and mutations in mammalian cell lines.

PAGES

753-755

Journal

TITLE

Nature Methods

ISSUE

9

VOLUME

8

Related Patents

  • Methods And Compositions For Increasing Efficiency Of Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Methods For Producing A Mouse Xy Embryonic (Es) Cell Line Capable Of Producing A Fertile Xy Female Mouse In An F0 Generation
  • Efficient Non-Meiotic Allele Introgression In Livestock
  • Methods For Increasing The Efficiency Of Homology Directed Repair (Hdr) In The Cellular Genome
  • Delivery, Engineering And Optimization Of Tandem Guide Systems, Methods And Compositions For Sequence Manipulation
  • Protected Guide Rnas (Pgrnas)
  • Targeted Modification Of Rat Genome
  • Methods And Compositions For The Targeted Modification Of A Mouse Genome
  • Methods For Genomic Integration
  • Optimized Crispr-Cas Double Nickase Systems, Methods And Compositions For Sequence Manipulation
  • Genome Editing By Crispr-Cas9 Using Short Donor Oligonucleotides
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Nuclease-Mediated Targeting With Large Targeting Vectors
  • Genetically Modified Animals And Methods For Making The Same
  • Optimization Of Circular Single Stranded Dna Using M13 Phage
  • Genome Editing Using Targeting Endonucleases And Single-Stranded Nucleic Acids
  • Methods And Compositions For Targeted Genetic Modification Using Paired Guide Rnas
  • Methods And Compositions For Increasing Efficiency Of Increased Efficiency Oftargeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Functional Genomics Using Crispr-Cas Systems, Compositions, Methods, Screens And Applications Thereof
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Crispr-Cas Systems, Crystal Structure And Uses Thereof
  • Efficient Non-Meiotic Allele Introgression
  • Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Crispr-Cas Component Systems, Methods And Compositions For Sequence Manipulation
  • Nuclease-Mediated Targeting With Large Targeting Vectors
  • Methods And Compositions For The Targeted Modification Of A Mouse Embryonic Stem Cell Genome
  • Gene Knockout Method
  • Delivery, Use And Therapeutic Applications Of The Crispr-Cas Systems And Compositions For Targeting Disorders And Diseases Using Viral Components
  • Compositions And Methods Of Use Of Crispr-Cas Systems In Nucleotide Repeat Disorders
  • Methods And Compositions For Targeted Genetic Modification Through Single-Step Multiple Targeting
  • Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Engineering And Optimization Of Improved Systems, Methods And Enzyme Compositions For Sequence Manipulation
  • Methods And Compositions For Increasing Efficiency Of Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Genome Editing Using Effector Oligonucleotides For Therapeutic Treatment
  • Methods And Compositions For Targeted Genetic Modifications And Methods Of Use
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Methods For Genomic Integration
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Methods For Genomic Integration
  • Method For Producing Mutant Filamentous Fungi
  • Methods And Compositions For Increasing Efficiency Of Targeted Gene Modification Using Oligonucleotide-Mediated Gene Repair
  • Large Gene Excision And Insertion
  • Methods And Compositions For The Targeted Modification Of A Mouse Es Cell Genome
  • Non-Meiotic Allele Introgression
  • Crispr Enzyme Mutations Reducing Off-Target Effects
  • Genome Editing Using Effector Oligonucleotides For Therapeutic Treatment
  • Efficient Non-Meiotic Allele Introgression
  • Non-Meiotic Allele Introgression
  • Compositions And Methods For The Treatment Of Hemoglobinopathies
  • Clinically Applicable Characterization Of Genetic Variants By Genome Editing
  • Methods For Genomic Integration
  • Delivery, Engineering And Optimization Of Systems, Methods And Compositions For Sequence Manipulation And Therapeutic Applications
  • Crispr-Cas Component Systems, Methods And Compositions For Sequence Manipulation
  • Genome Engineering
  • Delivery And Use Of The Crispr-Cas Systems, Vectors And Compositions For Hepatic Targeting And Therapy
  • Targeted Modification Of Rat Genome
  • Methods, Models, Systems, And Apparatus For Identifying Target Sequences For Cas Enzymes Or Crispr-Cas Systems For Target Sequences And Conveying Results Thereof
  • Systems, Methods And Compositions For Sequence Manipulation With Optimized Functional Crispr-Cas Systems
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1653

    DOI

    http://dx.doi.org/10.1038/nmeth.1653

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1046255083

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21765410


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Technology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Single-Stranded", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Deletion", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Targeting", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutagenesis, Insertional", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oligodeoxyribonucleotides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Point Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Zinc Fingers", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Fuqiang", 
            "id": "sg:person.01023453566.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023453566.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pruett-Miller", 
            "givenName": "Shondra M", 
            "id": "sg:person.0633166457.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633166457.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Yuping", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gjoka", 
            "givenName": "Monika", 
            "id": "sg:person.01206015366.68", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206015366.68"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Duda", 
            "givenName": "Katarzyna", 
            "id": "sg:person.01167201025.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167201025.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.266102.1", 
              "name": [
                "Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Taunton", 
            "givenName": "Jack", 
            "id": "sg:person.01030603436.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030603436.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Collingwood", 
            "givenName": "Trevor N", 
            "id": "sg:person.01042227251.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042227251.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark", 
              "id": "http://www.grid.ac/institutes/grid.5254.6", 
              "name": [
                "Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Frodin", 
            "givenName": "Morten", 
            "id": "sg:person.0656110026.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656110026.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Davis", 
            "givenName": "Gregory D", 
            "id": "sg:person.0645256326.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645256326.35"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature03556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040117781", 
              "https://doi.org/10.1038/nature03556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1562", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033826570", 
              "https://doi.org/10.1038/nbt.1562"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2011-07-17", 
        "datePublishedReg": "2011-07-17", 
        "description": "Single-stranded oligonucleotides are used as donor templates for zinc-finger nucleases to create targeted genomic deletions and mutations in mammalian cell lines.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nmeth.1653", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2518423", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "9", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "8"
          }
        ], 
        "keywords": [
          "zinc finger nucleases", 
          "mammalian cell lines", 
          "genome editing", 
          "genomic deletions", 
          "donor template", 
          "cell lines", 
          "ssDNA oligonucleotides", 
          "nuclease", 
          "oligonucleotide", 
          "deletion", 
          "mutations", 
          "editing", 
          "lines", 
          "template"
        ], 
        "name": "High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases", 
        "pagination": "753-755", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1046255083"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth.1653"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21765410"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth.1653", 
          "https://app.dimensions.ai/details/publication/pub.1046255083"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T20:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_533.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nmeth.1653"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1653'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1653'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1653'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1653'


     

    This table displays all metadata directly associated to this object as RDF triples.

    194 TRIPLES      21 PREDICATES      53 URIs      42 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth.1653 schema:about N088d1161260443ff9995560acf9f5d3c
    2 N28509219875940ac95faddd42e574c4c
    3 N36fc3e3e0e604fe9bfe77504fc9570c8
    4 N54ee3c4288dc470b932a9bde9a581d53
    5 N5cd0429fcfbc4e488f02470fbafbdb1c
    6 N9852d863e5d642c294c01a9581af0119
    7 N9a287faf5832486f9fbfb4cbb6344209
    8 Ne7ad5ca0f832467f9d8579504c7d519a
    9 Ne9d45b8cbccb49bd9a1949ae59410689
    10 Ned5503a91f384e398a787894f1d5692e
    11 Nf67896a4deb44c7ba594097258ac82e4
    12 anzsrc-for:06
    13 anzsrc-for:10
    14 anzsrc-for:11
    15 schema:author Nf2d06c35ed794ee5bcf277fdbbbdde3f
    16 schema:citation sg:pub.10.1038/nature03556
    17 sg:pub.10.1038/nbt.1562
    18 schema:datePublished 2011-07-17
    19 schema:datePublishedReg 2011-07-17
    20 schema:description Single-stranded oligonucleotides are used as donor templates for zinc-finger nucleases to create targeted genomic deletions and mutations in mammalian cell lines.
    21 schema:genre article
    22 schema:isAccessibleForFree true
    23 schema:isPartOf N4129c826dae64191913b20f3435af63c
    24 Nc14cb32e89704fe5a3faaecd8ca71e5e
    25 sg:journal.1033763
    26 schema:keywords cell lines
    27 deletion
    28 donor template
    29 editing
    30 genome editing
    31 genomic deletions
    32 lines
    33 mammalian cell lines
    34 mutations
    35 nuclease
    36 oligonucleotide
    37 ssDNA oligonucleotides
    38 template
    39 zinc finger nucleases
    40 schema:name High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
    41 schema:pagination 753-755
    42 schema:productId N04b19cbd683141348bed740c4828149e
    43 N8c7232a9e84248a4a49a4f85f987d36f
    44 Nd5a51dca33474fd5afcccecaf4240cf1
    45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046255083
    46 https://doi.org/10.1038/nmeth.1653
    47 schema:sdDatePublished 2022-11-24T20:54
    48 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    49 schema:sdPublisher Nfa376419c6764eaaa6523f33a61769f6
    50 schema:url https://doi.org/10.1038/nmeth.1653
    51 sgo:license sg:explorer/license/
    52 sgo:sdDataset articles
    53 rdf:type schema:ScholarlyArticle
    54 N04b19cbd683141348bed740c4828149e schema:name pubmed_id
    55 schema:value 21765410
    56 rdf:type schema:PropertyValue
    57 N088d1161260443ff9995560acf9f5d3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    58 schema:name Zinc Fingers
    59 rdf:type schema:DefinedTerm
    60 N0eea0d61f2a849a2aed48f2cf52c0d44 rdf:first sg:person.0633166457.12
    61 rdf:rest Nb2f6e69b8e4f47ea8d82d04453a8dc6b
    62 N1b7b92b6bed54f34b0238d0ca25b13d9 rdf:first sg:person.01030603436.13
    63 rdf:rest N29bf4876dc1c4ca8ab23750759b51919
    64 N28509219875940ac95faddd42e574c4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    65 schema:name Cell Line
    66 rdf:type schema:DefinedTerm
    67 N29bf4876dc1c4ca8ab23750759b51919 rdf:first sg:person.01042227251.89
    68 rdf:rest Nc8a0711fe6f3479795ec67975ef0df5a
    69 N36fc3e3e0e604fe9bfe77504fc9570c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name Mutagenesis, Insertional
    71 rdf:type schema:DefinedTerm
    72 N4129c826dae64191913b20f3435af63c schema:volumeNumber 8
    73 rdf:type schema:PublicationVolume
    74 N54ee3c4288dc470b932a9bde9a581d53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name Oligodeoxyribonucleotides
    76 rdf:type schema:DefinedTerm
    77 N58e20b96b69d447cab1d4bc55319d7d1 rdf:first sg:person.01206015366.68
    78 rdf:rest N82aded320c45412bac34519abcebc957
    79 N5cd0429fcfbc4e488f02470fbafbdb1c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Gene Deletion
    81 rdf:type schema:DefinedTerm
    82 N82aded320c45412bac34519abcebc957 rdf:first sg:person.01167201025.59
    83 rdf:rest N1b7b92b6bed54f34b0238d0ca25b13d9
    84 N8c7232a9e84248a4a49a4f85f987d36f schema:name doi
    85 schema:value 10.1038/nmeth.1653
    86 rdf:type schema:PropertyValue
    87 N926fb2026c764b0cb6622fb911d28416 rdf:first sg:person.0645256326.35
    88 rdf:rest rdf:nil
    89 N9852d863e5d642c294c01a9581af0119 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Gene Targeting
    91 rdf:type schema:DefinedTerm
    92 N9a287faf5832486f9fbfb4cbb6344209 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Point Mutation
    94 rdf:type schema:DefinedTerm
    95 Nb247a83ffdb44272acd9f8d4ade666c9 schema:affiliation grid-institutes:None
    96 schema:familyName Huang
    97 schema:givenName Yuping
    98 rdf:type schema:Person
    99 Nb2f6e69b8e4f47ea8d82d04453a8dc6b rdf:first Nb247a83ffdb44272acd9f8d4ade666c9
    100 rdf:rest N58e20b96b69d447cab1d4bc55319d7d1
    101 Nc14cb32e89704fe5a3faaecd8ca71e5e schema:issueNumber 9
    102 rdf:type schema:PublicationIssue
    103 Nc8a0711fe6f3479795ec67975ef0df5a rdf:first sg:person.0656110026.88
    104 rdf:rest N926fb2026c764b0cb6622fb911d28416
    105 Nd5a51dca33474fd5afcccecaf4240cf1 schema:name dimensions_id
    106 schema:value pub.1046255083
    107 rdf:type schema:PropertyValue
    108 Ne7ad5ca0f832467f9d8579504c7d519a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Humans
    110 rdf:type schema:DefinedTerm
    111 Ne9d45b8cbccb49bd9a1949ae59410689 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Genetic Engineering
    113 rdf:type schema:DefinedTerm
    114 Ned5503a91f384e398a787894f1d5692e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Endonucleases
    116 rdf:type schema:DefinedTerm
    117 Nf2d06c35ed794ee5bcf277fdbbbdde3f rdf:first sg:person.01023453566.64
    118 rdf:rest N0eea0d61f2a849a2aed48f2cf52c0d44
    119 Nf67896a4deb44c7ba594097258ac82e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name DNA, Single-Stranded
    121 rdf:type schema:DefinedTerm
    122 Nfa376419c6764eaaa6523f33a61769f6 schema:name Springer Nature - SN SciGraph project
    123 rdf:type schema:Organization
    124 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Biological Sciences
    126 rdf:type schema:DefinedTerm
    127 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
    128 schema:name Technology
    129 rdf:type schema:DefinedTerm
    130 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Medical and Health Sciences
    132 rdf:type schema:DefinedTerm
    133 sg:grant.2518423 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1653
    134 rdf:type schema:MonetaryGrant
    135 sg:journal.1033763 schema:issn 1548-7091
    136 1548-7105
    137 schema:name Nature Methods
    138 schema:publisher Springer Nature
    139 rdf:type schema:Periodical
    140 sg:person.01023453566.64 schema:affiliation grid-institutes:None
    141 schema:familyName Chen
    142 schema:givenName Fuqiang
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023453566.64
    144 rdf:type schema:Person
    145 sg:person.01030603436.13 schema:affiliation grid-institutes:grid.266102.1
    146 schema:familyName Taunton
    147 schema:givenName Jack
    148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030603436.13
    149 rdf:type schema:Person
    150 sg:person.01042227251.89 schema:affiliation grid-institutes:None
    151 schema:familyName Collingwood
    152 schema:givenName Trevor N
    153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042227251.89
    154 rdf:type schema:Person
    155 sg:person.01167201025.59 schema:affiliation grid-institutes:grid.5254.6
    156 schema:familyName Duda
    157 schema:givenName Katarzyna
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167201025.59
    159 rdf:type schema:Person
    160 sg:person.01206015366.68 schema:affiliation grid-institutes:None
    161 schema:familyName Gjoka
    162 schema:givenName Monika
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206015366.68
    164 rdf:type schema:Person
    165 sg:person.0633166457.12 schema:affiliation grid-institutes:None
    166 schema:familyName Pruett-Miller
    167 schema:givenName Shondra M
    168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633166457.12
    169 rdf:type schema:Person
    170 sg:person.0645256326.35 schema:affiliation grid-institutes:None
    171 schema:familyName Davis
    172 schema:givenName Gregory D
    173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645256326.35
    174 rdf:type schema:Person
    175 sg:person.0656110026.88 schema:affiliation grid-institutes:grid.5254.6
    176 schema:familyName Frodin
    177 schema:givenName Morten
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656110026.88
    179 rdf:type schema:Person
    180 sg:pub.10.1038/nature03556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040117781
    181 https://doi.org/10.1038/nature03556
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1038/nbt.1562 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033826570
    184 https://doi.org/10.1038/nbt.1562
    185 rdf:type schema:CreativeWork
    186 grid-institutes:None schema:alternateName Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA
    187 schema:name Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA
    188 rdf:type schema:Organization
    189 grid-institutes:grid.266102.1 schema:alternateName Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA
    190 schema:name Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA
    191 rdf:type schema:Organization
    192 grid-institutes:grid.5254.6 schema:alternateName Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark
    193 schema:name Biotech Research and Innovation Centre and Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark
    194 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...