Megapixel digital PCR View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-08

AUTHORS

Kevin A Heyries, Carolina Tropini, Michael VanInsberghe, Callum Doolin, Oleh I Petriv, Anupam Singhal, Kaston Leung, Curtis B Hughesman, Carl L Hansen

ABSTRACT

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number. More... »

PAGES

649

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1640

DOI

http://dx.doi.org/10.1038/nmeth.1640

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021013575

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21725299


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