Chemically defined conditions for human iPSC derivation and culture View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-05

AUTHORS

Guokai Chen, Daniel R Gulbranson, Zhonggang Hou, Jennifer M Bolin, Victor Ruotti, Mitchell D Probasco, Kimberly Smuga-Otto, Sara E Howden, Nicole R Diol, Nicholas E Propson, Ryan Wagner, Garrett O Lee, Jessica Antosiewicz-Bourget, Joyce M C Teng, James A Thomson

ABSTRACT

We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods. More... »

PAGES

424

Journal

TITLE

Nature Methods

ISSUE

5

VOLUME

8

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1593

    DOI

    http://dx.doi.org/10.1038/nmeth.1593

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028183020

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21478862


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