A transgenic mouse for in vivo detection of endogenous labeled mRNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-02

AUTHORS

Timothée Lionnet, Kevin Czaplinski, Xavier Darzacq, Yaron Shav-Tal, Amber L Wells, Jeffrey A Chao, Hye Yoon Park, Valeria de Turris, Melissa Lopez-Jones, Robert H Singer

ABSTRACT

Live-cell single mRNA imaging is a powerful tool but has been restricted in higher eukaryotes to artificial cell lines and reporter genes. We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generated a knock-in mouse line with an MS2 binding site (MBS) cassette targeted to the 3' untranslated region of the essential β-actin gene. As β-actin-MBS was ubiquitously expressed, we could uniquely address endogenous mRNA regulation in any tissue or cell type. We simultaneously followed transcription from the β-actin alleles in real time and observed transcriptional bursting in response to serum stimulation with precise temporal resolution. We tracked single endogenous labeled mRNA particles being transported in primary hippocampal neurons. The MBS cassette also enabled high-sensitivity fluorescence in situ hybridization (FISH), allowing detection and localization of single β-actin mRNA molecules in various mouse tissues. More... »

PAGES

165

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1551

DOI

http://dx.doi.org/10.1038/nmeth.1551

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046539154

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21240280


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