Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-08

AUTHORS

Mazhar Adli, Jiang Zhu, Bradley E Bernstein

ABSTRACT

Current methods for whole-genome mapping of protein-DNA interactions, performed by coupling chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), require large amounts of starting materials, which precludes their application to rare cell types. Here we combine a high-sensitivity ChIP assay with a new library preparation procedure to map histone modifications in as few as 10,000 cells. We used the technique to characterize mouse hematopoietic progenitors and thereby gain insight into their developmental program. More... »

PAGES

615

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1478

DOI

http://dx.doi.org/10.1038/nmeth.1478

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050226586

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20622861


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