Ontology type: schema:ScholarlyArticle
2010-07
AUTHORSDaniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi, Fabio Beltram
ABSTRACTChloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments. More... »
PAGES516
http://scigraph.springernature.com/pub.10.1038/nmeth.1471
DOIhttp://dx.doi.org/10.1038/nmeth.1471
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/20581829
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