Simultaneous intracellular chloride and pH measurements using a GFP-based sensor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-07

AUTHORS

Daniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi, Fabio Beltram

ABSTRACT

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments. More... »

PAGES

516

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1471

DOI

http://dx.doi.org/10.1038/nmeth.1471

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029326756

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20581829


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