Enhanced neuronal RNAi in C. elegans using SID-1 View Full Text


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Article Info

DATE

2010-07

AUTHORS

Andrea Calixto, Dattananda Chelur, Irini Topalidou, Xiaoyin Chen, Martin Chalfie

ABSTRACT

We expressed SID-1, a transmembrane protein from Caenorhabditis elegans that is required for systemic RNA interference (RNAi), in C. elegans neurons. This expression increased the response of neurons to double-stranded (ds)RNA delivered by feeding. Mutations in the lin-15b and lin-35 genes enhanced this effect. Worms expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. Neuronal expression of sid-1 decreased nonneuronal RNAi, suggesting that neurons expressing transgenic sid-1(+) served as a sink for dsRNA. This effect, or a sid-1(-) background, can be used to uncover neuronal defects for lethal genes. Expression of sid-1(+) from cell-specific promoters in sid-1 mutants results in cell-specific feeding RNAi. We used these strains to identify a role for integrin signaling genes in mechanosensation. More... »

PAGES

554

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1463

DOI

http://dx.doi.org/10.1038/nmeth.1463

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032555226

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20512143


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