Targeted gene deletions in C. elegans using transposon excision View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-06

AUTHORS

Christian Frøkjær-Jensen, M Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G Moerman, Erik M Jorgensen

ABSTRACT

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker. More... »

PAGES

451

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1454

DOI

http://dx.doi.org/10.1038/nmeth.1454

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023315310

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20418868


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