A nonviral minicircle vector for deriving human iPS cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-03

AUTHORS

Fangjun Jia, Kitchener D Wilson, Ning Sun, Deepak M Gupta, Mei Huang, Zongjin Li, Nicholas J Panetta, Zhi Ying Chen, Robert C Robbins, Mark A Kay, Michael T Longaker, Joseph C Wu

ABSTRACT

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. More... »

PAGES

197

Journal

TITLE

Nature Methods

ISSUE

3

VOLUME

7

Author Affiliations

Related Patents

  • Induced Pluripotent Stem Cells With Synthetic Modified Rnas
  • Kit For Making Induced Pluripotent Stem Cells Using Modified Rnas
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Engineered Nucleic Acids Encoding A Modified Erythropoietin And Their Expression
  • Improved Transposon System For Gene Delivery
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides Encoding Granulysin
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Enriched Population Of Human Pluripotent Cells With Oct-4 And Sox2 Integrated Into Their Genome
  • Compositions, Kits, And Methods For Making Induced Pluripotent Stem Cells Using Synthetic Modified Rnas
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Septin-4
  • Kits Comprising Linear Dnas For Sustained Polypeptide Expression Using Synthetic, Modified Rnas
  • Method Of Making Induced Pluripotent Stem Cell From Adipose Stem Cells Using Minicircle Dna Vectors
  • Modified Polynucleotides For Treating Protein Deficiency
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Hepatocyte-Like Cells And Uses Thereof
  • Plasmid For Minicircle Production
  • Generation Of Induced Pluripotent Stem Cells From Small Volumes Of Peripheral Blood
  • Methods And Materials For Obtaining Induced Pluripotent Stem Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • In Vitro Differentiation Of Pluripotent Stem Cells To Pancreatic Endoderm Cells (Pec) And Endocrine Cells
  • Method And Apparatus For Reprogramming Of Living Cells
  • Enhanced Efficiency Of Induced Pluripotent Stem Cell Generation
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Formulation And Delivery Of Plga Microspheres
  • Compositions And Methods Of Altering Cholesterol Levels
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Modified Polynucleotides For The Production Of Proteins
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Modified Polynucleotides For The Production Of G-Csf
  • Methods For The Production Of Ips Cells Using Non-Viral Approach
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Plasmid For Minicircle Production
  • Reprogramming Immortalized B-Cells To Induced Pluripotent Stem Cells
  • Terminally Modified Rna
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Methods For The Production Of Ips Cells
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Method For De-Differentiating A Cell
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • In Vivo Production Of Proteins
  • Modified Polynucleotides For The Production Of Proteins
  • Method For Producing A Protein Of Interest In A Primate
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Split Dose Administration
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1426

    DOI

    http://dx.doi.org/10.1038/nmeth.1426

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019714920

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20139967


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adult", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Circular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Induced Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jia", 
            "givenName": "Fangjun", 
            "id": "sg:person.015640223117.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015640223117.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.", 
                "Department of Bioengineering, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wilson", 
            "givenName": "Kitchener D", 
            "id": "sg:person.011041300147.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011041300147.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sun", 
            "givenName": "Ning", 
            "id": "sg:person.0736366403.92", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736366403.92"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Department of Surgery, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gupta", 
            "givenName": "Deepak M", 
            "id": "sg:person.0630216235.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630216235.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Mei", 
            "id": "sg:person.01057440335.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057440335.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Zongjin", 
            "id": "sg:person.01073412370.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073412370.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Department of Surgery, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Panetta", 
            "givenName": "Nicholas J", 
            "id": "sg:person.0675664611.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675664611.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Zhi Ying", 
            "id": "sg:person.01247574762.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247574762.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Robbins", 
            "givenName": "Robert C", 
            "id": "sg:person.0613536505.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613536505.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kay", 
            "givenName": "Mark A", 
            "id": "sg:person.01164322460.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164322460.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.", 
                "Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Longaker", 
            "givenName": "Michael T", 
            "id": "sg:person.0603442164.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603442164.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Stanford University", 
              "id": "https://www.grid.ac/institutes/grid.168010.e", 
              "name": [
                "Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.", 
                "Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Joseph C", 
            "id": "sg:person.012641221217.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012641221217.65"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.stem.2009.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000666854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07864", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002136274", 
              "https://doi.org/10.1038/nature07864"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0805802105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002283811"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1525-0016(03)00168-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003365119"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1525-0016(03)00168-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003365119"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2009.04.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006373679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.191.10.1649", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006510978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0908450106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007416274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0908450106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007416274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0003474", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009196519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2008.03.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010434590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymeth.2008.03.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010434590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019109862", 
              "https://doi.org/10.1038/nature05944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.02.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019496424"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2007-0843", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019747395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07863", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019816783", 
              "https://doi.org/10.1038/nature07863"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1162494", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021871278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcp.20463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023602527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tibtech.2006.01.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038665949"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1383", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039633163", 
              "https://doi.org/10.1038/nbt1383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1172482", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044377678"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e02-02-0105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048841479"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051466228", 
              "https://doi.org/10.1038/nbt.1502"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.2005.16.126", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059272468"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/0022-1317-72-11-2727", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060357778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1164270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062458687"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06337.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082656364"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-03", 
        "datePublishedReg": "2010-03-01", 
        "description": "Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nmeth.1426", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2608321", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6848983", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6849222", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2668890", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2613966", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2686591", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2608280", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2355284", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2691987", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1033763", 
            "issn": [
              "1548-7091", 
              "1548-7105"
            ], 
            "name": "Nature Methods", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "name": "A nonviral minicircle vector for deriving human iPS cells", 
        "pagination": "197", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "5def2dbe078fb41f9638dccbd7ced66de06699e2d8aff4b37ef439df46360e58"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "20139967"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101215604"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nmeth.1426"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1019714920"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nmeth.1426", 
          "https://app.dimensions.ai/details/publication/pub.1019714920"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T09:53", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89793_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nmeth.1426"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'


     

    This table displays all metadata directly associated to this object as RDF triples.

    277 TRIPLES      21 PREDICATES      61 URIs      27 LITERALS      15 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nmeth.1426 schema:about N15ca23f4c4f4492f90ed6c4d2358d2b4
    2 N7883f098d5174e2a871229a859136024
    3 N8e629d2dd7b94600ad6b9f6cacd13c20
    4 Naaa6bc38cd244e96ba2579e29acba5cc
    5 Nce5bf49286bd4bb887953292683c7737
    6 Ne930b74d7b0741da9e795ef4bf7ff62f
    7 anzsrc-for:06
    8 anzsrc-for:0604
    9 schema:author N29c9815124e74a68a6058b856cbcdf47
    10 schema:citation sg:pub.10.1038/nature05934
    11 sg:pub.10.1038/nature05944
    12 sg:pub.10.1038/nature07863
    13 sg:pub.10.1038/nature07864
    14 sg:pub.10.1038/nbt.1502
    15 sg:pub.10.1038/nbt1383
    16 https://doi.org/10.1002/j.1460-2075.1994.tb06337.x
    17 https://doi.org/10.1002/jcp.20463
    18 https://doi.org/10.1016/j.cell.2009.02.013
    19 https://doi.org/10.1016/j.stem.2007.05.014
    20 https://doi.org/10.1016/j.stem.2009.04.005
    21 https://doi.org/10.1016/j.stem.2009.05.005
    22 https://doi.org/10.1016/j.tibtech.2006.01.010
    23 https://doi.org/10.1016/j.ymeth.2008.03.006
    24 https://doi.org/10.1016/s1525-0016(03)00168-0
    25 https://doi.org/10.1073/pnas.0805802105
    26 https://doi.org/10.1073/pnas.0908450106
    27 https://doi.org/10.1084/jem.191.10.1649
    28 https://doi.org/10.1089/hum.2005.16.126
    29 https://doi.org/10.1091/mbc.e02-02-0105
    30 https://doi.org/10.1099/0022-1317-72-11-2727
    31 https://doi.org/10.1126/science.1162494
    32 https://doi.org/10.1126/science.1164270
    33 https://doi.org/10.1126/science.1172482
    34 https://doi.org/10.1371/journal.pone.0003474
    35 https://doi.org/10.1634/stemcells.2007-0843
    36 schema:datePublished 2010-03
    37 schema:datePublishedReg 2010-03-01
    38 schema:description Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
    39 schema:genre research_article
    40 schema:inLanguage en
    41 schema:isAccessibleForFree true
    42 schema:isPartOf N5364c4684b794cd0b48343778ed0ee8e
    43 N8b452a9a47cd4635b589099c42a1994b
    44 sg:journal.1033763
    45 schema:name A nonviral minicircle vector for deriving human iPS cells
    46 schema:pagination 197
    47 schema:productId N418ba451c5c945aa8d5b542f4fdb4dd1
    48 N5e1bf79cb72641fd84974ae1eb1c475e
    49 N73ad6d4c59d84ea3ae2d2a4a6e7826e3
    50 Nbd5ab2256fbd49928b30922fa21dee94
    51 Ndba7575278114da2a059d202c26fb171
    52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019714920
    53 https://doi.org/10.1038/nmeth.1426
    54 schema:sdDatePublished 2019-04-11T09:53
    55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    56 schema:sdPublisher Nc00e7d0ad6744b9fa804b4a3454d07a1
    57 schema:url https://www.nature.com/articles/nmeth.1426
    58 sgo:license sg:explorer/license/
    59 sgo:sdDataset articles
    60 rdf:type schema:ScholarlyArticle
    61 N15ca23f4c4f4492f90ed6c4d2358d2b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    62 schema:name Genetic Vectors
    63 rdf:type schema:DefinedTerm
    64 N2238c98d7512443b9c0ebd314874210c rdf:first sg:person.0613536505.26
    65 rdf:rest N28c1c05b8b1b435e82e2209bb399b8d4
    66 N23a994fb14884979956fb37e84f20dbe rdf:first sg:person.012641221217.65
    67 rdf:rest rdf:nil
    68 N24603c302a6140ceb5f97a3f386cd1af rdf:first sg:person.011041300147.05
    69 rdf:rest N944ba9f08a714652b949f6b2df2881b3
    70 N28c1c05b8b1b435e82e2209bb399b8d4 rdf:first sg:person.01164322460.34
    71 rdf:rest Nfe4845eacc8d44f5a144079eb448f799
    72 N29c9815124e74a68a6058b856cbcdf47 rdf:first sg:person.015640223117.38
    73 rdf:rest N24603c302a6140ceb5f97a3f386cd1af
    74 N418ba451c5c945aa8d5b542f4fdb4dd1 schema:name doi
    75 schema:value 10.1038/nmeth.1426
    76 rdf:type schema:PropertyValue
    77 N5364c4684b794cd0b48343778ed0ee8e schema:issueNumber 3
    78 rdf:type schema:PublicationIssue
    79 N5e1bf79cb72641fd84974ae1eb1c475e schema:name dimensions_id
    80 schema:value pub.1019714920
    81 rdf:type schema:PropertyValue
    82 N5f1ed0bda8784ceea87daa3fd1c8ff03 rdf:first sg:person.01247574762.32
    83 rdf:rest N2238c98d7512443b9c0ebd314874210c
    84 N644e72f1ba1f4449a9f7298aa4410a7d rdf:first sg:person.0630216235.94
    85 rdf:rest N8714892ad92d44e4a96c0420942f1864
    86 N73ad6d4c59d84ea3ae2d2a4a6e7826e3 schema:name nlm_unique_id
    87 schema:value 101215604
    88 rdf:type schema:PropertyValue
    89 N7883f098d5174e2a871229a859136024 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Adult
    91 rdf:type schema:DefinedTerm
    92 N8714892ad92d44e4a96c0420942f1864 rdf:first sg:person.01057440335.79
    93 rdf:rest Nbad1a480ced44befa64489a4c9043635
    94 N8b452a9a47cd4635b589099c42a1994b schema:volumeNumber 7
    95 rdf:type schema:PublicationVolume
    96 N8e629d2dd7b94600ad6b9f6cacd13c20 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Induced Pluripotent Stem Cells
    98 rdf:type schema:DefinedTerm
    99 N944ba9f08a714652b949f6b2df2881b3 rdf:first sg:person.0736366403.92
    100 rdf:rest N644e72f1ba1f4449a9f7298aa4410a7d
    101 Naaa6bc38cd244e96ba2579e29acba5cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name DNA, Circular
    103 rdf:type schema:DefinedTerm
    104 Nb44709d3830e4d8c93bbad2c8632f7d2 rdf:first sg:person.0675664611.45
    105 rdf:rest N5f1ed0bda8784ceea87daa3fd1c8ff03
    106 Nbad1a480ced44befa64489a4c9043635 rdf:first sg:person.01073412370.56
    107 rdf:rest Nb44709d3830e4d8c93bbad2c8632f7d2
    108 Nbd5ab2256fbd49928b30922fa21dee94 schema:name readcube_id
    109 schema:value 5def2dbe078fb41f9638dccbd7ced66de06699e2d8aff4b37ef439df46360e58
    110 rdf:type schema:PropertyValue
    111 Nc00e7d0ad6744b9fa804b4a3454d07a1 schema:name Springer Nature - SN SciGraph project
    112 rdf:type schema:Organization
    113 Nce5bf49286bd4bb887953292683c7737 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Humans
    115 rdf:type schema:DefinedTerm
    116 Ndba7575278114da2a059d202c26fb171 schema:name pubmed_id
    117 schema:value 20139967
    118 rdf:type schema:PropertyValue
    119 Ne930b74d7b0741da9e795ef4bf7ff62f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Transfection
    121 rdf:type schema:DefinedTerm
    122 Nfe4845eacc8d44f5a144079eb448f799 rdf:first sg:person.0603442164.05
    123 rdf:rest N23a994fb14884979956fb37e84f20dbe
    124 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Biological Sciences
    126 rdf:type schema:DefinedTerm
    127 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    128 schema:name Genetics
    129 rdf:type schema:DefinedTerm
    130 sg:grant.2355284 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    131 rdf:type schema:MonetaryGrant
    132 sg:grant.2608280 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    133 rdf:type schema:MonetaryGrant
    134 sg:grant.2608321 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    135 rdf:type schema:MonetaryGrant
    136 sg:grant.2613966 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    137 rdf:type schema:MonetaryGrant
    138 sg:grant.2668890 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    139 rdf:type schema:MonetaryGrant
    140 sg:grant.2686591 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    141 rdf:type schema:MonetaryGrant
    142 sg:grant.2691987 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    143 rdf:type schema:MonetaryGrant
    144 sg:grant.6848983 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    145 rdf:type schema:MonetaryGrant
    146 sg:grant.6849222 http://pending.schema.org/fundedItem sg:pub.10.1038/nmeth.1426
    147 rdf:type schema:MonetaryGrant
    148 sg:journal.1033763 schema:issn 1548-7091
    149 1548-7105
    150 schema:name Nature Methods
    151 rdf:type schema:Periodical
    152 sg:person.01057440335.79 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    153 schema:familyName Huang
    154 schema:givenName Mei
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057440335.79
    156 rdf:type schema:Person
    157 sg:person.01073412370.56 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    158 schema:familyName Li
    159 schema:givenName Zongjin
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073412370.56
    161 rdf:type schema:Person
    162 sg:person.011041300147.05 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    163 schema:familyName Wilson
    164 schema:givenName Kitchener D
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011041300147.05
    166 rdf:type schema:Person
    167 sg:person.01164322460.34 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    168 schema:familyName Kay
    169 schema:givenName Mark A
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164322460.34
    171 rdf:type schema:Person
    172 sg:person.01247574762.32 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    173 schema:familyName Chen
    174 schema:givenName Zhi Ying
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247574762.32
    176 rdf:type schema:Person
    177 sg:person.012641221217.65 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    178 schema:familyName Wu
    179 schema:givenName Joseph C
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012641221217.65
    181 rdf:type schema:Person
    182 sg:person.015640223117.38 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    183 schema:familyName Jia
    184 schema:givenName Fangjun
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015640223117.38
    186 rdf:type schema:Person
    187 sg:person.0603442164.05 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    188 schema:familyName Longaker
    189 schema:givenName Michael T
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603442164.05
    191 rdf:type schema:Person
    192 sg:person.0613536505.26 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    193 schema:familyName Robbins
    194 schema:givenName Robert C
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613536505.26
    196 rdf:type schema:Person
    197 sg:person.0630216235.94 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    198 schema:familyName Gupta
    199 schema:givenName Deepak M
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630216235.94
    201 rdf:type schema:Person
    202 sg:person.0675664611.45 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    203 schema:familyName Panetta
    204 schema:givenName Nicholas J
    205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675664611.45
    206 rdf:type schema:Person
    207 sg:person.0736366403.92 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
    208 schema:familyName Sun
    209 schema:givenName Ning
    210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736366403.92
    211 rdf:type schema:Person
    212 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    213 https://doi.org/10.1038/nature05934
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1038/nature05944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019109862
    216 https://doi.org/10.1038/nature05944
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1038/nature07863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019816783
    219 https://doi.org/10.1038/nature07863
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1038/nature07864 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002136274
    222 https://doi.org/10.1038/nature07864
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1038/nbt.1502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466228
    225 https://doi.org/10.1038/nbt.1502
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1038/nbt1383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039633163
    228 https://doi.org/10.1038/nbt1383
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1002/j.1460-2075.1994.tb06337.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082656364
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1002/jcp.20463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023602527
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/j.cell.2009.02.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019496424
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1016/j.stem.2009.04.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006373679
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1016/j.stem.2009.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000666854
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/j.tibtech.2006.01.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038665949
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1016/j.ymeth.2008.03.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010434590
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1016/s1525-0016(03)00168-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003365119
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1073/pnas.0805802105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002283811
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1073/pnas.0908450106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007416274
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1084/jem.191.10.1649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006510978
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1089/hum.2005.16.126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272468
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1091/mbc.e02-02-0105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048841479
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1099/0022-1317-72-11-2727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060357778
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1126/science.1162494 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021871278
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1126/science.1164270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062458687
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1126/science.1172482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044377678
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1371/journal.pone.0003474 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009196519
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1634/stemcells.2007-0843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019747395
    269 rdf:type schema:CreativeWork
    270 https://www.grid.ac/institutes/grid.168010.e schema:alternateName Stanford University
    271 schema:name Department of Bioengineering, Stanford University School of Medicine, Stanford, California, USA.
    272 Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, California, USA.
    273 Department of Surgery, Stanford University School of Medicine, Stanford, California, USA.
    274 Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.
    275 Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA.
    276 Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA.
    277 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...