A nonviral minicircle vector for deriving human iPS cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-03

AUTHORS

Fangjun Jia, Kitchener D Wilson, Ning Sun, Deepak M Gupta, Mei Huang, Zongjin Li, Nicholas J Panetta, Zhi Ying Chen, Robert C Robbins, Mark A Kay, Michael T Longaker, Joseph C Wu

ABSTRACT

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. More... »

PAGES

197

Journal

TITLE

Nature Methods

ISSUE

3

VOLUME

7

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1426

    DOI

    http://dx.doi.org/10.1038/nmeth.1426

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019714920

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20139967


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    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nmeth.1426'

    RDF/XML is a standard XML format for linked data.

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