Target-enrichment strategies for next-generation sequencing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-02

AUTHORS

Lira Mamanova, Alison J Coffey, Carol E Scott, Iwanka Kozarewa, Emily H Turner, Akash Kumar, Eleanor Howard, Jay Shendure, Daniel J Turner

ABSTRACT

We have not yet reached a point at which routine sequencing of large numbers of whole eukaryotic genomes is feasible, and so it is often necessary to select genomic regions of interest and to enrich these regions before sequencing. There are several enrichment approaches, each with unique advantages and disadvantages. Here we describe our experiences with the leading target-enrichment technologies, the optimizations that we have performed and typical results that can be obtained using each. We also provide detailed protocols for each technology so that end users can find the best compromise between sensitivity, specificity and uniformity for their particular project. More... »

PAGES

111

References to SciGraph publications

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  • Journal

    TITLE

    Nature Methods

    ISSUE

    2

    VOLUME

    7

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1419

    DOI

    http://dx.doi.org/10.1038/nmeth.1419

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1042265948

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20111037


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