Parallel, tag-directed assembly of locally derived short sequence reads View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-02

AUTHORS

Joseph B Hiatt, Rupali P Patwardhan, Emily H Turner, Choli Lee, Jay Shendure

ABSTRACT

We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing. More... »

PAGES

119

Journal

TITLE

Nature Methods

ISSUE

2

VOLUME

7

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1416

    DOI

    http://dx.doi.org/10.1038/nmeth.1416

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025089276

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20081835


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