High-throughput generation of selected reaction-monitoring assays for proteins and proteomes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-01

AUTHORS

Paola Picotti, Oliver Rinner, Robert Stallmach, Franziska Dautel, Terry Farrah, Bruno Domon, Holger Wenschuh, Ruedi Aebersold

ABSTRACT

Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases. More... »

PAGES

43-46

Journal

TITLE

Nature Methods

ISSUE

1

VOLUME

7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1408

DOI

http://dx.doi.org/10.1038/nmeth.1408

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022418066

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19966807


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