Universal sample preparation method for proteome analysis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05

AUTHORS

Jacek R Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj, Matthias Mann

ABSTRACT

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage. More... »

PAGES

359-362

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1322

DOI

http://dx.doi.org/10.1038/nmeth.1322

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047379825

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19377485


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