Enzymatic assembly of DNA molecules up to several hundred kilobases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-04-12

AUTHORS

Daniel G Gibson, Lei Young, Ray-Yuan Chuang, J Craig Venter, Clyde A Hutchison, Hamilton O Smith

ABSTRACT

The combination of 5′ exonuclease, DNA polymerase and ligase with overlapping DNA fragments facilitates the in vitro assembly of large DNA constructs, including an entire bacterial genome.

PAGES

343-345

References to SciGraph publications

  • 1995-04. PCR-mediated recombination and mutagenesis in MOLECULAR BIOTECHNOLOGY
  • 2005-11-24. Foundations for engineering biology in NATURE
  • 2007-11-25. Gene synthesis by circular assembly amplification in NATURE METHODS
  • 2007-02-11. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC in NATURE METHODS
  • 2008-04-14. Engineering BioBrick vectors from BioBrick parts in JOURNAL OF BIOLOGICAL ENGINEERING
  • Journal

    TITLE

    Nature Methods

    ISSUE

    5

    VOLUME

    6

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1318

    DOI

    http://dx.doi.org/10.1038/nmeth.1318

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040405189

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19363495


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