miRNA in situ hybridization in formaldehyde and EDC–fixed tissues View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-02

AUTHORS

John T G Pena, Cherin Sohn-Lee, Sara H Rouhanifard, Janos Ludwig, Markus Hafner, Aleksandra Mihailovic, Cindy Lim, Daniel Holoch, Philipp Berninger, Mihaela Zavolan, Thomas Tuschl

ABSTRACT

MicroRNAs are small regulatory RNAs with many biological functions and disease associations. We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide that irreversibly immobilizes the microRNA at its 5' phosphate. We determined optimal hybridization parameters for 130 locked nucleic acid probes by recording nucleic acid melting temperature during ISH. More... »

PAGES

139-141

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1294

DOI

http://dx.doi.org/10.1038/nmeth.1294

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041669030

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19137005


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