An in vitro microfluidic approach to generating protein-interaction networks View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-01

AUTHORS

Doron Gerber, Sebastian J Maerkl, Stephen R Quake

ABSTRACT

We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways. More... »

PAGES

71-74

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1289

DOI

http://dx.doi.org/10.1038/nmeth.1289

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017721691

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19098921


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