A large genome center's improvements to the Illumina sequencing system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-12

AUTHORS

Michael A Quail, Iwanka Kozarewa, Frances Smith, Aylwyn Scally, Philip J Stephens, Richard Durbin, Harold Swerdlow, Daniel J Turner

ABSTRACT

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data. More... »

PAGES

1005-1010

References to SciGraph publications

Journal

TITLE

Nature Methods

ISSUE

12

VOLUME

5

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nmeth.1270

DOI

http://dx.doi.org/10.1038/nmeth.1270

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021189255

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19034268


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