Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-03

AUTHORS

Micah Hamady, Jeffrey J Walker, J Kirk Harris, Nicholas J Gold, Rob Knight

ABSTRACT

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing. More... »

PAGES

235-237

Journal

TITLE

Nature Methods

ISSUE

3

VOLUME

5

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nmeth.1184

    DOI

    http://dx.doi.org/10.1038/nmeth.1184

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18264105


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