Colocalization of retrovirus and target cells on specific fibronectin fragments increases genetic transduction of mammalian cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-08

AUTHORS

H Hanenberg, X L Xiao, D Dilloo, K Hashino, I Kato, D A Williams

ABSTRACT

Hematopoietic cells are important targets for genetic modification with retroviral vectors. Attempts at human gene therapy of stem cells have achieved limited success partly because of low gene transfer efficiency. Chymotryptic fragments of the extracellular matrix molecule fibronectin used during infection have been shown to increase transduction of human hematopoietic progenitor cells. Here, we demonstrate that this enhanced gene transfer into mammalian target cells is due to direct binding of retroviral particles to sequences within the fibronectin molecule. Transduction of mammalian cells, including murine long-term repopulating hematopoietic cells, is greatly enhanced when cells are adherent to chimeric fragments containing these retroviral binding sequences. In addition, colocalization of retrovirus and target cells on fibronectin peptides allows targeted transduction of specific cell types by exploiting unique ligand/receptor interactions. More... »

PAGES

876-882

Journal

TITLE

Nature Medicine

ISSUE

8

VOLUME

2

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nm0896-876

    DOI

    http://dx.doi.org/10.1038/nm0896-876

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8705856


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